bay-80-6946 and Prostatic-Neoplasms

bay-80-6946 has been researched along with Prostatic-Neoplasms* in 1 studies

Other Studies

1 other study(ies) available for bay-80-6946 and Prostatic-Neoplasms

Article	Year
A mass spectrometric method for in-depth profiling of phosphoinositide regioisomers and their disease-associated regulation. Nature communications, 2022, 01-10, Volume: 13, Issue:1 Phosphoinositides are a family of membrane lipids essential for many biological and pathological processes. Due to the existence of multiple phosphoinositide regioisomers and their low intracellular concentrations, profiling these lipids and linking a specific acyl variant to a change in biological state have been difficult. To enable the comprehensive analysis of phosphoinositide phosphorylation status and acyl chain identity, we develop PRMC-MS (Phosphoinositide Regioisomer Measurement by Chiral column chromatography and Mass Spectrometry). Using this method, we reveal a severe skewing in acyl chains in phosphoinositides in Pten-deficient prostate cancer tissues, extracellular mobilization of phosphoinositides upon expression of oncogenic PIK3CA, and a unique profile for exosomal phosphoinositides. Thus, our approach allows characterizing the dynamics of phosphoinositide acyl variants in intracellular and extracellular milieus. Topics: Animals; Chromatography, Affinity; Class I Phosphatidylinositol 3-Kinases; Epidermal Growth Factor; Exosomes; Gene Expression; HEK293 Cells; HeLa Cells; Humans; Male; Mass Spectrometry; Metabolome; Mice; PC-3 Cells; Phosphatidylinositols; Prostate; Prostatic Neoplasms; PTEN Phosphohydrolase; Pyrimidines; Quinazolines; Stereoisomerism	2022

Article

Year

A mass spectrometric method for in-depth profiling of phosphoinositide regioisomers and their disease-associated regulation.

Nature communications, 2022, 01-10, Volume: 13, Issue:1

Phosphoinositides are a family of membrane lipids essential for many biological and pathological processes. Due to the existence of multiple phosphoinositide regioisomers and their low intracellular concentrations, profiling these lipids and linking a specific acyl variant to a change in biological state have been difficult. To enable the comprehensive analysis of phosphoinositide phosphorylation status and acyl chain identity, we develop PRMC-MS (Phosphoinositide Regioisomer Measurement by Chiral column chromatography and Mass Spectrometry). Using this method, we reveal a severe skewing in acyl chains in phosphoinositides in Pten-deficient prostate cancer tissues, extracellular mobilization of phosphoinositides upon expression of oncogenic PIK3CA, and a unique profile for exosomal phosphoinositides. Thus, our approach allows characterizing the dynamics of phosphoinositide acyl variants in intracellular and extracellular milieus.

Topics: Animals; Chromatography, Affinity; Class I Phosphatidylinositol 3-Kinases; Epidermal Growth Factor; Exosomes; Gene Expression; HEK293 Cells; HeLa Cells; Humans; Male; Mass Spectrometry; Metabolome; Mice; PC-3 Cells; Phosphatidylinositols; Prostate; Prostatic Neoplasms; PTEN Phosphohydrolase; Pyrimidines; Quinazolines; Stereoisomerism

2022