bay-11-7082 and DNA-Virus-Infections

The budded virus (BV) of Antheraea pernyi is a nuclear polyhedrosis virus (ApNPV), that belongs to the baculovirus family. It is capable of stimulating innate immune response in avian macrophage-like cells (HD11). In this study, we used the expression of proinflammatory cytokines (IFN-gamma and IL-12p40) and production of nitric oxide (NO) as indicators to evaluate the involvement of the signaling cascades in the activation of HD11 cells by BV. Western blot analysis revealed that ERK1/2, p38 MAPK and JNK were phosphorylated followed by activation of p65-NF-kappaB after HD11 cells were challenged with BV. Inhibition of p38 MAPK and NF-kappaB by their respective inhibitors abolished BV-induced expression of IL-12p40, IFN-gamma and production of NO, whereas inhibition of JNK abolished only the induction of cytokines. Additionally, inhibition of ERK1/2 resulted in unexpected increases in both cytokine expression and NO production. Taken together, these findings indicated that in BV-induced HD11 cells, the induction of cytokines was mediated by the p38 MAPK, JNK and NF-kappaB pathways, and NO production was mediated through the p38 MAPK/NF-kappaB pathways.

Topics: Animals; Anthracenes; Birds; Cell Line; DNA Virus Infections; Imidazoles; Immunity, Innate; Interferon-gamma; Interleukin-12 Subunit p40; Macrophage Activation; Macrophages; MAP Kinase Kinase 4; MAP Kinase Signaling System; Moths; Nitric Oxide; Nitriles; Nucleopolyhedroviruses; p38 Mitogen-Activated Protein Kinases; Phosphorylation; Pyridines; Sulfones; Virulence