bafilomycin-a1 has been researched along with Progeria* in 1 studies
1 other study(ies) available for bafilomycin-a1 and Progeria
Article | Year |
---|---|
ESCRT-III controls nuclear envelope deformation induced by progerin.
Hutchinson-Gilford progeria syndrome (HGPS) is a premature aging disorder, caused by mutation in the gene encoding lamin A/C, which produces a truncated protein called progerin. In cells from HGPS patients, progerin accumulates at the nuclear membrane (NM), where it causes NM deformations. In this study, we investigated whether progerin-induced NM deformation involved ESCRT-III, a protein complex that remodels nuclear and cytoplasmic membranes. The ESCRT-III protein CHMP4B was recruited to sites of aberrant NM proliferation in human cells ectopically expressing progerin and in patient-derived HGPS fibroblasts. Derepression of NM deformation in these cells was observed following depletion of CHMP4B or an ESCRT-III adaptor, ALIX. Treatment with rapamycin (which induce autophagic clearance of progerin and reverse progerin-induced cellular phenotypes) down-regulated progerin-induced NM deformation, whereas treatment with bafilomycin A1 (an inhibitor of autophagy and lysosome-based degradation) or CHMP4B depletion antagonized the effects of rapamycin. These results indicate that the ALIX-mediated ESCRT-III pathway plays a suppressive role in progerin-induced NM deformation and suggest that autophagy down-regulates progerin-induced NM deformation in a manner dependent on ESCRT-III machinery. Topics: Aging; Calcium-Binding Proteins; Cell Cycle Proteins; Cell Line; Cell Nucleus; Cell Proliferation; Endosomal Sorting Complexes Required for Transport; Fibroblasts; Humans; Lamin Type A; Macrolides; Mutation; Nuclear Envelope; Progeria; Sirolimus | 2020 |