bafilomycin-a and Nerve-Degeneration

bafilomycin-a has been researched along with Nerve-Degeneration* in 2 studies

Other Studies

2 other study(ies) available for bafilomycin-a and Nerve-Degeneration

ArticleYear
Boosting chaperone-mediated autophagy in vivo mitigates α-synuclein-induced neurodegeneration.
    Brain : a journal of neurology, 2013, Volume: 136, Issue:Pt 7

    α-Synuclein levels are critical to Parkinson's disease pathogenesis. Wild-type α-synuclein is degraded partly by chaperone-mediated autophagy, and aberrant α-synuclein may act as an inhibitor of the pathway. To address whether the induction of chaperone-mediated autophagy may represent a potential therapy against α-synuclein-induced neurotoxicity, we overexpressed lysosomal-associated membrane protein 2a, the rate-limiting step of chaperone-mediated autophagy, in human neuroblastoma SH-SY5Y cells, rat primary cortical neurons in vitro, and nigral dopaminergic neurons in vivo. Overexpression of the lysosomal-associated membrane protein 2a in cellular systems led to upregulation of chaperone-mediated autophagy, decreased α-synuclein turnover, and selective protection against adenoviral-mediated wild-type α-synuclein neurotoxicity. Protection was observed even when the steady-state levels of α-synuclein were unchanged, suggesting that it occurred through the attenuation of α-synuclein-mediated dysfunction of chaperone-mediated autophagy. Overexpression of the lysosomal receptor through the nigral injection of recombinant adeno-associated virus vectors effectively ameliorated α-synuclein-induced dopaminergic neurodegeneration by increasing the survival of neurons located in the substantia nigra as well as the axon terminals located in the striatum, which was associated with a reduction in total α-synuclein levels and related aberrant species. We conclude that induction of chaperone-mediated autophagy may provide a novel therapeutic strategy in Parkinson's disease and related synucleinopathies through two different mechanisms: amelioration of dysfunction of chaperone-mediated autophagy and lowering of α-synuclein levels.

    Topics: alpha-Synuclein; Amphetamine; Analysis of Variance; Animals; Apomorphine; Autophagy; Cells, Cultured; Cerebral Cortex; Corpus Striatum; Dependovirus; Dopamine; Embryo, Mammalian; Enzyme Inhibitors; Female; Gene Expression Regulation; Genetic Vectors; Green Fluorescent Proteins; Hemagglutinins; Humans; Lysosomal Membrane Proteins; Lysosomal-Associated Membrane Protein 2; Macrolides; Mice; Molecular Chaperones; Motor Activity; Nerve Degeneration; Neuroblastoma; Neurons; Rats; Transfection; Tyrosine 3-Monooxygenase

2013
Low-dose bafilomycin attenuates neuronal cell death associated with autophagy-lysosome pathway dysfunction.
    Journal of neurochemistry, 2010, Volume: 114, Issue:4

    We have shown previously that the plecomacrolide antibiotics bafilomycin A1 and B1 significantly attenuate cerebellar granule neuron death resulting from agents that disrupt lysosome function. To further characterize bafilomycin-mediated cytoprotection, we examined its ability to attenuate the death of naïve and differentiated neuronal SH-SY5Y human neuroblastoma cells from agents that induce lysosome dysfunction in vitro, and from in vivo dopaminergic neuron death in C. elegans. Low-dose bafilomycin significantly attenuated SH-SY5Y cell death resulting from treatment with chloroquine, hydroxychloroquine amodiaquine and staurosporine. Bafilomycin also attenuated the chloroquine-induced reduction in processing of cathepsin D, the principal lysosomal aspartic acid protease, to its mature 'active' form. Chloroquine induced autophagic vacuole accumulation and inhibited autophagic flux, effects that were attenuated upon treatment with bafilomycin and were associated with a significant decrease in chloroquine-induced accumulation of detergent-insoluble alpha-synuclein oligomers. In addition, bafilomycin significantly and dose-dependently attenuated dopaminergic neuron death in C. elegans resulting from in vivo over-expression of human wild-type alpha-synuclein. Together, our findings suggest that low-dose bafilomycin is cytoprotective in part through its maintenance of the autophagy-lysosome pathway, and underscores its therapeutic potential for treating Parkinson's disease and other neurodegenerative diseases that exhibit disruption of protein degradation pathways and accumulation of toxic protein species.

    Topics: Animals; Autophagy; Caenorhabditis elegans; Cell Line, Tumor; Cytoprotection; Disease Progression; Humans; Lysosomes; Macrolides; Nerve Degeneration; Neurons; Signal Transduction

2010