bacoside-a3 and Disease-Models--Animal

Bacopa monniera Wettst. (BM, syn. Herpestis monniera L; Scrophulariaceae), is an Ayurvedic drug used as a rasayana. Its fresh juice was earlier reported to have significant antiulcerogenic activity. In continuation, methanolic extract of BM (BME) standardized to bacoside-A content (percentage-38.0 +/- 0.9), when given in the dose of 10-50 mg/kg, twice daily for 5 days, showed dose-dependent anti-ulcerogenic on various gastric ulcer models induced by ethanol, aspirin, 2 h cold restraint stress and 4 h pylorus ligation. BME in the dose of 20 mg/kg, given for 10 days, twice daily showed healing effects against 50% acetic acid-induced gastric ulcers. Further work was done to investigate the possible mechanisms of its action by studying its effect on various mucosal offensive acid-pepsin secretion and defensive factors like mucin secretion, mucosal cell shedding, cell proliferation and antioxidant activity in rats. BME 20 mg/kg showed no effect on acid-pepsin secretion, increased mucin secretion, while it decreased cell shedding with no effect on cell proliferation. BME showed significant antioxidant effect per se and in stressed animals. Thus, the gastric prophylactic and curative effects of BME may be due to its predominant effect on mucosal defensive factors.

Topics: Acetic Acid; Animals; Antioxidants; Aspirin; Catalase; Cell Division; Cold Temperature; Disease Models, Animal; Dose-Response Relationship, Drug; Ethanol; Female; Gastric Juice; Gastric Mucosa; Lipid Peroxidation; Male; Mucins; Pepsin A; Phytotherapy; Plant Extracts; Rats; Rats, Inbred Strains; Saponins; Scrophulariaceae; Stomach; Stomach Ulcer; Sucralfate; Superoxide Dismutase; Triterpenes; Wound Healing