bacillomycin-d and Staphylococcal-Infections

The aim of this study was to assess antibacterial activity of a novel Bacillus velezensis strain NST6, and further identify its active compound against pathogenic Staphylococcus strains for clinical therapeutic applications.. In this study, a novel B. velezensis strain NST6 harbouring strong antimicrobial activity against human pathogenic bacteria was isolated from a soil sample. The solvent extract of the strain exhibited strong antibacterial activity against Gram-positive and Gram-negative bacteria in disc diffusion assay and measurement of minimal inhibitory concentration and bactericidal concentration, of which it showed notable efficacy to Staphylococcus species including Staphylococcus epidermidis, Staphylococcus aureus and methicillin-resistant S. aureus. Strong antibacterial effect against pathogenic S. aureus and low toxicity of the bacterial extract were further validated in Caenorhabditis elegans model. Moreover, by antibacterial activity-guided fractionation using RP-HPLC and LC-MS, we defined C. The primary anti-staphylococcal compound from B. velezensis NST6 was identified as a cyclic lipopeptide, C. We provide an alternative treatment option to Staphylococcus infections by investigating the specific anti-staphylococcal activity of C

Topics: Anti-Bacterial Agents; Antimicrobial Cationic Peptides; Bacillus; Humans; Lipopeptides; Methicillin-Resistant Staphylococcus aureus; Microbial Sensitivity Tests; Peptides, Cyclic; Staphylococcal Infections; Staphylococcus