azd-6244 and Thyroid-Carcinoma--Anaplastic

Background: Cancer stem cells (CSCs) with a self-renewal ability in tumor cells population, execute a pivotal\ function in tumorigenesis, retrogression, and metastasis of malignant cancers such as anaplastic thyroid carcinoma\ (ATC). Materials and Methods: In this study, we isolated CSCs subpopulation with CD133 surface marker from\ three ATC cell lines by magnetic cell sorting assay. After confirming the segregation by the flow cytometry method,\ BRAF and sodium-iodide symporter (NIS) genes were investigated in them before and after incubation with BRAF\ inhibitor. Also, we evaluated the NIS protein expression and localization. Results: Established upon q-RT PCR data,\ when compared to human normal thyrocytes, the BRAFV600E gene was over-expressed in CD133pos cells (>1705.99 ±\ 55.55 fold, Mean ± SEM, n=3, P- value<0.05), whilst the expression of NIS gene was very restricted (< 0.0008 ± 5.43\ fold, Mean ± SEM, n=3, P- value<0.05) in them. Also, our results showed that BRAF inhibition affected NIS protein\ expression and localization. Conclusions: Current study showed that the differentiate genes/proteins expression can\ be induced in the CSCs via focus on signal transduction pathways and targeting their molecules, that are involved in\ expression of these genes/proteins. Therefore, attention to targeting CSCs along with routine thyroid cancer therapy,\ can help to ATC treatment.

Topics: AC133 Antigen; Benzimidazoles; Cell Proliferation; Cells, Cultured; Gene Expression Regulation, Neoplastic; Humans; Neoplastic Stem Cells; Proto-Oncogene Proteins B-raf; Signal Transduction; Symporters; Thyroid Carcinoma, Anaplastic; Thyroid Gland; Thyroid Neoplasms