azd-6244 and Metaplasia

azd-6244 has been researched along with Metaplasia* in 2 studies

Other Studies

2 other study(ies) available for azd-6244 and Metaplasia

Article	Year
MEK Inhibitor Reverses Metaplasia and Allows Re-Emergence of Normal Lineages in Helicobacter pylori-Infected Gerbils. Gastroenterology, 2019, Volume: 156, Issue:3 Topics: Acrylonitrile; Aniline Compounds; Animals; Benzimidazoles; Biopsy, Needle; Disease Models, Animal; Gastric Mucosa; Gerbillinae; Helicobacter Infections; Helicobacter pylori; Immunohistochemistry; Male; Metaplasia; Random Allocation; Reference Values; Treatment Outcome	2019
Expression of Activated Ras in Gastric Chief Cells of Mice Leads to the Full Spectrum of Metaplastic Lineage Transitions. Gastroenterology, 2016, Volume: 150, Issue:4 Gastric cancer develops in the context of parietal cell loss, spasmolytic polypeptide-expressing metaplasia (SPEM), and intestinal metaplasia (IM). We investigated whether expression of the activated form of Ras in gastric chief cells of mice leads to the development of SPEM, as well as progression of metaplasia.. We studied Mist1-CreERT2Tg/+;LSL-K-Ras(G12D)Tg/+ (Mist1-Kras) mice, which express the active form of Kras in chief cells on tamoxifen exposure. We studied Mist1-CreERT2Tg/+;LSL-KRas (G12D)Tg/+;R26RmTmG/+ (Mist1-Kras-mTmG) mice to examine whether chief cells that express active Kras give rise to SPEM and IM. Some mice received intraperitoneal injections of the Mitogen-activated protein kinase kinase (MEK) inhibitor, selumetinib, for 14 consecutive days. Gastric tissues were collected and analyzed by immunohistochemistry, immunofluorescence, and quantitative polymerase chain reaction.. Mist1-Kras mice developed metaplastic glands, which completely replaced normal fundic lineages and progressed to IM within 3-4 months after tamoxifen injection. The metaplastic glands expressed markers of SPEM and IM, and were infiltrated by macrophages. Lineage tracing studies confirmed that the metaplasia developed directly from Kras (G12D)-induced chief cells. Selumetinib induced persistent regression of SPEM and IM, and re-established normal mucosal cells, which were derived from normal gastric progenitor cells.. Expression of activated Ras in chief cells of Mist1-Kras mice led to the full range of metaplastic lineage transitions, including SPEM and IM. Inhibition of Ras signaling by inhibition of MEK might reverse preneoplastic metaplasia in the stomach. Topics: Animals; Anticarcinogenic Agents; Benzimidazoles; Cell Differentiation; Cell Lineage; Cell Proliferation; Cell Transformation, Neoplastic; Chief Cells, Gastric; Disease Progression; Female; Gene Expression Regulation, Neoplastic; Genes, ras; Genetic Predisposition to Disease; Humans; Macrophages; Male; Metaplasia; Mice, Inbred C57BL; Mice, Transgenic; Mitogen-Activated Protein Kinase Kinases; Mutation; Phenotype; Protein Kinase Inhibitors; Signal Transduction; Stomach Neoplasms; Time Factors; Transcriptional Activation	2016

Article

Year

MEK Inhibitor Reverses Metaplasia and Allows Re-Emergence of Normal Lineages in Helicobacter pylori-Infected Gerbils.

Gastroenterology, 2019, Volume: 156, Issue:3

Topics: Acrylonitrile; Aniline Compounds; Animals; Benzimidazoles; Biopsy, Needle; Disease Models, Animal; Gastric Mucosa; Gerbillinae; Helicobacter Infections; Helicobacter pylori; Immunohistochemistry; Male; Metaplasia; Random Allocation; Reference Values; Treatment Outcome

2019

Expression of Activated Ras in Gastric Chief Cells of Mice Leads to the Full Spectrum of Metaplastic Lineage Transitions.

Gastroenterology, 2016, Volume: 150, Issue:4

Gastric cancer develops in the context of parietal cell loss, spasmolytic polypeptide-expressing metaplasia (SPEM), and intestinal metaplasia (IM). We investigated whether expression of the activated form of Ras in gastric chief cells of mice leads to the development of SPEM, as well as progression of metaplasia.. We studied Mist1-CreERT2Tg/+;LSL-K-Ras(G12D)Tg/+ (Mist1-Kras) mice, which express the active form of Kras in chief cells on tamoxifen exposure. We studied Mist1-CreERT2Tg/+;LSL-KRas (G12D)Tg/+;R26RmTmG/+ (Mist1-Kras-mTmG) mice to examine whether chief cells that express active Kras give rise to SPEM and IM. Some mice received intraperitoneal injections of the Mitogen-activated protein kinase kinase (MEK) inhibitor, selumetinib, for 14 consecutive days. Gastric tissues were collected and analyzed by immunohistochemistry, immunofluorescence, and quantitative polymerase chain reaction.. Mist1-Kras mice developed metaplastic glands, which completely replaced normal fundic lineages and progressed to IM within 3-4 months after tamoxifen injection. The metaplastic glands expressed markers of SPEM and IM, and were infiltrated by macrophages. Lineage tracing studies confirmed that the metaplasia developed directly from Kras (G12D)-induced chief cells. Selumetinib induced persistent regression of SPEM and IM, and re-established normal mucosal cells, which were derived from normal gastric progenitor cells.. Expression of activated Ras in chief cells of Mist1-Kras mice led to the full range of metaplastic lineage transitions, including SPEM and IM. Inhibition of Ras signaling by inhibition of MEK might reverse preneoplastic metaplasia in the stomach.

Topics: Animals; Anticarcinogenic Agents; Benzimidazoles; Cell Differentiation; Cell Lineage; Cell Proliferation; Cell Transformation, Neoplastic; Chief Cells, Gastric; Disease Progression; Female; Gene Expression Regulation, Neoplastic; Genes, ras; Genetic Predisposition to Disease; Humans; Macrophages; Male; Metaplasia; Mice, Inbred C57BL; Mice, Transgenic; Mitogen-Activated Protein Kinase Kinases; Mutation; Phenotype; Protein Kinase Inhibitors; Signal Transduction; Stomach Neoplasms; Time Factors; Transcriptional Activation

2016