azaguanine and Lymphoma

The studies reported in this communication were conducted to determine if naturally occurring cell constituents could themselves cause mutation. All the bases and their corresponding nucleosides have been shown in culture to produce chromosome damage in P388 mouse lymphoma and Chinese hamster ovary cells (CHO) and adenosine has produced an increased incidence of sister chromatid exchanges in CHO cells. In addition thymidine has produced an increase in V79 cells resistant to 8-azaguanine and ouabain, although no effects were seen in six strains of Salmonella typhimurium. Such damage probably arises as a result of imbalanced DNA-precursor pools. Thus mutagenic events may arise by mechanisms unrelated to direct alterations of DNA.

Topics: Adenosine; Animals; Azaguanine; Cell Line; Cell Survival; Chromosomes; Cricetinae; Drug Resistance; Female; Lymphoma; Mice; Mutagenicity Tests; Mutation; Nucleosides; Ouabain; Ovary; Salmonella typhimurium; Sister Chromatid Exchange; Thymidine

Topics: Animals; Azaguanine; Bromodeoxyuridine; Cell Line; Cell Survival; Genetic Variation; Idoxuridine; Lymphoma; Mice; Mutagens; Thymidine; Time Factors

Topics: Animals; Azaguanine; Cell Line; Cell Survival; Genetic Variation; Idoxuridine; Lymphoma; Methotrexate; Mice; Mutagens; Mutation; Selection, Genetic; Time Factors

Topics: Animals; Azaguanine; Caffeine; Cell Line; Cell Survival; Cells, Cultured; Cricetinae; DNA Repair; DNA Replication; Drug Resistance; Female; Lung; Lymphoma; Mice; Mutation; Ovary; Probability; Radiation Genetics; Sarcoma, Yoshida; Time Factors; Ultraviolet Rays

13 clonal cell lines were isolated from a hybrid cell population established by cell fusion between cloned BALB/c myeloma cells that were resistant to 8-azaguanine and produced immunoglobulin G and free kappa chain and C(67)BL/6n lymphoma cells that were resistant to bromodeoxyuridine and did not produce immunoglobulins. Some of the histocompatibility-2 antigens of both parental cell lines could be demonstrated on all of the hybrid clones. 11 clones synthesized only free kappa chain. Two clones synthesized IgG of BALB/c type, as well as free kappa chain. These clones had higher chromosome number than did the clones that synthesized only kappa chains.

Topics: Animals; Antigens; Azaguanine; Bromodeoxyuridine; Cell Fusion; Cell Line; Clone Cells; Complement System Proteins; Culture Media; Histocompatibility; Hybrid Cells; Immune Sera; Immunodiffusion; Immunoelectrophoresis; Immunoglobulin G; Immunoglobulins; Karyotyping; Lymphoma; Mice; Multiple Myeloma; Rabbits; Species Specificity

Topics: Animals; Azaguanine; Cell Count; Cell Line; Chromosomes; Culture Techniques; Depression, Chemical; Hydrocortisone; Immunoglobulins; Leucine; Lymphoma; Mice; Neoplasm Transplantation; Plasmacytoma; Thymidine; Tritium

Topics: Animals; Azaguanine; Clone Cells; Culture Media; Culture Techniques; DNA, Neoplasm; Glycine; Hypoxanthines; Lymphoma; Methotrexate; Mice; Neoplasms, Experimental; Nucleotides; Phosphotransferases; Purines; Thymidine

Topics: Alkylating Agents; Animals; Antineoplastic Agents; Azaguanine; Azaserine; Carcinoma, Ehrlich Tumor; Carcinoma, Hepatocellular; DNA; DNA, Neoplasm; Fluorouracil; Idoxuridine; Liver Neoplasms; Lymphoma; Lymphoma, Non-Hodgkin; Mercaptopurine; Mice; Neoplasms, Experimental; Nitrogen Mustard Compounds; Nucleosides; Nucleotides; Purines; Research; RNA; RNA, Neoplasm; Sarcoma 180; Thioguanine; Uracil Mustard

Topics: Antineoplastic Agents; Azaguanine; Cortisone; Cyclophosphamide; Humans; Lymphoma; Mercaptopurine; Mitomycin; Mitomycins; Nitrogen Mustard Compounds; Prednisolone; Thiotepa; Triethylenemelamine

Topics: Animals; Antineoplastic Agents; Azaguanine; Fluorouracil; Leukemia; Leukemia, Lymphoid; Lymphoma; Methotrexate; Mice; Nucleotides; Pyrimidines

Topics: Animals; Antineoplastic Agents; Azaguanine; Guanine; Guanosine Monophosphate; Lymphoma; Mice; Neoplasms; Rats