azaguanine and Carcinoma--Hepatocellular

Topics: Animals; Azacitidine; Azaguanine; Carcinoma, Hepatocellular; Cells, Cultured; Cytidine; Dactinomycin; Electrophoresis, Polyacrylamide Gel; Fluorouracil; Guanosine; Isotope Labeling; Liver; Liver Neoplasms; Neoplasms, Experimental; Rats; RNA, Neoplasm; RNA, Ribosomal; Spectrophotometry, Ultraviolet; Time Factors; Tritium; Uridine

Topics: Animals; Azacitidine; Azaguanine; Carbon Radioisotopes; Carcinoma, Hepatocellular; Electrophoresis, Polyacrylamide Gel; Guanosine; Liver Neoplasms; Methionine; Neoplasms, Experimental; Rats; RNA, Neoplasm; RNA, Ribosomal; Transcription, Genetic; Tritium; Uridine

Topics: Aminopterin; Animals; Azaguanine; Carcinoma, Hepatocellular; Cell Fusion; Cell Line; Chromosomes; Clone Cells; Culture Media; Drug Resistance; Humans; Hybrid Cells; Hypoxanthines; Karyotyping; Liver Neoplasms; Lung; Parainfluenza Virus 1, Human; Pentosyltransferases; Rats; Thymidine

Topics: Adrenal Glands; Adrenalectomy; Age Factors; Animals; Azacitidine; Azaguanine; Carbon Isotopes; Carboxy-Lyases; Carcinoma, Hepatocellular; Cycloheximide; Dactinomycin; DNA; Enzyme Induction; Hepatectomy; Hydrocortisone; Liver; Liver Neoplasms; Liver Regeneration; Male; Ornithine; Rats; Solubility

Topics: Animals; Anti-Bacterial Agents; Antimetabolites; Azaguanine; Carbon Isotopes; Carcinoma, Hepatocellular; Cell Nucleus; Centrifugation, Density Gradient; Cycloheximide; Cytoplasm; Depression, Chemical; Feedback; Leucine; Liver; Liver Neoplasms; Male; Metabolism; Neoplasms, Experimental; Orotic Acid; Puromycin; Pyrrolidines; Rats; Ribosomes; RNA; Stimulation, Chemical; Time Factors; Tryptophan

1. The ribosomal components in the postmitochondrial supernatant of a rat hepatoma (hepatoma 7800) and the corresponding host liver were examined for diversity and functional competence. 2. The ;free' and ;membrane-bound' polyribosomes of both tissues were equally active in vivo and had equilibrated with newly synthesized ribosomes 4hr. after administration of [6-(14)C]orotic acid. 3. The inactive monomer-dimer pool in hepatoma 7800 was unattached to membranes and a larger fraction of the polyribosomes was free in hepatoma than in liver. 4. By using sensitivity to puromycin as a criterion, evidence was obtained that most of the polyribosomes in hepatoma 7800 were active in vivo. 5. Actinomycin, azaguanine and carbon tetrachloride caused marked conversion of polyribosomes into inactive monomers and dimers in the host liver and moderate conversion in the hepatoma. 6. Significant accumulation of ferritin and shifts in the mean polyribosome size to the lighter species occurred in the host liver of rats bearing large hepatomas.

Topics: Animals; Azaguanine; Carbon Isotopes; Carbon Tetrachloride; Carcinoma, Hepatocellular; Dactinomycin; Ferritins; Liver; Liver Neoplasms; Neoplasms, Experimental; Orotic Acid; Puromycin; Rats; Ribosomes

Topics: Animals; Azaguanine; Carbon Isotopes; Carcinoma, Hepatocellular; Dactinomycin; Liver; Liver Neoplasms; Male; Neoplasms, Experimental; Orotic Acid; Rats; Ribosomes; RNA, Messenger

Topics: Alkylating Agents; Animals; Antineoplastic Agents; Azaguanine; Azaserine; Carcinoma, Ehrlich Tumor; Carcinoma, Hepatocellular; DNA; DNA, Neoplasm; Fluorouracil; Idoxuridine; Liver Neoplasms; Lymphoma; Lymphoma, Non-Hodgkin; Mercaptopurine; Mice; Neoplasms, Experimental; Nitrogen Mustard Compounds; Nucleosides; Nucleotides; Purines; Research; RNA; RNA, Neoplasm; Sarcoma 180; Thioguanine; Uracil Mustard