atrial-natriuretic-factor and Leukemia--Monocytic--Acute

In a survey for unknown bioactive peptides in mammalian cell lines, we isolated peptides exhibiting a strong relaxant effect on chick rectum from a phorbol ester-supplemented culture medium of the human monocytic cell line, THP-1. The peptide was deduced to be a C-terminal 29-residue peptide derived from a human C-type natriuretic peptide (CNP) precursor. CNP mRNA was also detected in the THP-1 cells, and expression of CNP gene and CNP concentration in the culture medium was found to be highly augmented by the stimulation of phorbol ester. Production of CNP in THP-1 cells suggests that CNP also functions as a local regulator in the blood cell-vascular system, although CNP has previously been recognized as a neuropeptide functioning in the central nervous system.

Topics: Amino Acid Sequence; Animals; Atrial Natriuretic Factor; Chickens; Chromatography, Gel; Chromatography, High Pressure Liquid; Humans; In Vitro Techniques; Leukemia, Monocytic, Acute; Molecular Sequence Data; Muscle Relaxation; Muscle, Smooth; Protein Precursors; Rectum; RNA, Messenger; Tetradecanoylphorbol Acetate; Tumor Cells, Cultured