atractylenolide-i and Skin-Neoplasms

In this study, we aimed to investigate the anti-melanoma effects and the JAK2/STAT3 pathway-related mechanism of action of atractylenolide I in human melanoma cells. Our results showed that atractylenolide I effectively reduced viability, induced apoptosis and inhibited migration of melanoma cells. Meanwhile, atractylenolide I decreased the protein expression levels of phospho-JAK2 and phospho-STAT3, and in turn downregulated the mRNA levels of STAT3-targeted genes, including Bcl-xL, MMP-2 and MMP-9. Furthermore, the cytotoxic effect of atractylenolide I was attenuated in STAT3-overactivated A375 cells. These findings indicate that inhibition of JAK2/STAT3 signalling contributes to the anti-melanoma effects of atractylenolide I.

Topics: Apoptosis; Cell Line, Tumor; Cell Movement; Cell Survival; Down-Regulation; Gene Expression Profiling; Humans; Janus Kinase 2; Lactones; Melanoma; Phosphorylation; RNA, Messenger; Sesquiterpenes; Signal Transduction; Skin Neoplasms; STAT3 Transcription Factor