astragalin and Breast-Neoplasms

Breast cancer is the most aggressive malignant tumor with high morbidity and mortality. Astragalin, a flavonoid widely found in a variety of edible and medicinal plants, is recorded to possess multiple biological and pharmacological activities. However, its effect of anti-breast cancer has been unknown.. Computational pharmacology was employed to explore the potential mechanism of anti-metastasis and anti-angiogenesis effects of Astragalin on breast cancer. The targets of Astragalin were obtained from TCMSP, Swiss Target Prediction, SEA, BATMAN-TCM, ChemMapper and STITCH databases, and targets of breast cancer were got from OMIM, GeneCards, and DisGeNET databases. Protein-protein interaction network (PPI), Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed to elucidate the interactions of these two groups of targets. Moreover, the anti-metastasis and anti-angiogenesis effects of Astragalin were validated by in vitro and in vivo experiments using wound healing assay, transwell migration and invasion assay, gelatin zymography assay, tube formation assay, and chick embryo chorioallantoic membrane model.. Computational pharmacology analysis indicated that the effects of Astragalin against breast cancer were mainly related to the regulation of the cell movement, migration, and angiogenesis, and taking AKT, ZEB1, VEGF, and MMP9 as the promising targets. Further experimental pharmacology indicated that Astragalin exerted anti-metastasis and anti-angiogenesis activities on breast cancer, and verified AKT, ZEB1, VEGF, and MMP9 as the key targets.. Astragalin suppresses the metastasis and angiogenesis of breast cancer, and AKT, ZEB1, VEGF, and MMP9 are the promising targets for Astragalin against breast cancer. Thus, Astragalin is a potential therapeutic agent for breast cancer.

Topics: Angiogenesis Inhibitors; Animals; Breast Neoplasms; Chick Embryo; Drugs, Chinese Herbal; Female; Humans; Matrix Metalloproteinase 9; Molecular Docking Simulation; Proto-Oncogene Proteins c-akt; Vascular Endothelial Growth Factor A

Yellow camellia, with its golden yellow flowers, is rare in the world. Most studies of yellow camellia have focused on its ornamental properties; however, there are fewer published studies on its medical values. The purpose of this study was to define the chemical constituents and the biological potential of the water extract of leaves in six species of yellow camellia. The data showed that Camellia murauchii had significantly higher total catechins and total polyphenol content than others; Camellia euphlebia had the highest total amino acids and γ-aminobutyric acid. The results indicated that Camellia tunghinensis exhibited the highest free radical scavenging capacity and showed potent anticancer activities. Camellia nitidissima had stronger inhibitory effect than other species on fatty acid synthesis. In addition to catechins, 3-p-coumaroylquinic acid, kaempferol-3-O-glucoside, and quercetin-3-O-glucoside were detected in C. tunghinensis using liquid chromatography-tandem mass spectrometry. Taken together, yellow camellias possess biological activity and are worthy of continued study.

Topics: Amino Acids; Antineoplastic Agents, Phytogenic; Antioxidants; Breast Neoplasms; Caffeine; Camellia; Catechin; Cell Line, Tumor; Cell Survival; Chromatography, High Pressure Liquid; Chromatography, Liquid; Female; Flavonoids; Flowers; gamma-Aminobutyric Acid; Glucosides; Humans; Kaempferols; Monosaccharides; Plant Extracts; Plant Leaves; Polyphenols; Quercetin; Tandem Mass Spectrometry