astatine and Stomach-Neoplasms

Tissue factor (TF), the trigger protein of the extrinsic blood coagulation cascade, is abundantly expressed in various cancers including gastric cancer. Anti-TF monoclonal antibodies (mAbs) capable of targeting cancers have been successfully applied to armed antibodies such as antibody-drug conjugates (ADCs) and molecular imaging probes. We prepared an anti-TF mAb, clone 1084, labeled with astatine-211 (

Topics: Animals; Antibodies, Monoclonal, Humanized; Ascorbic Acid; Astatine; Blood Coagulation; Body Weight; Cell Line, Tumor; Female; Heterografts; Humans; Immunoconjugates; Linear Energy Transfer; Mice; Mice, Inbred BALB C; Mice, Nude; Protein Denaturation; Radiation-Protective Agents; Radioimmunotherapy; Receptor, ErbB-2; Stomach Neoplasms; Thromboplastin

We investigated the local concentration of α-particles from

Topics: Alpha Particles; Animals; Antibodies, Monoclonal; Astatine; Isotope Labeling; Linear Energy Transfer; Liver Neoplasms; Mice; Radiometry; Stomach Neoplasms

The histopathological changes of subcutaneous heterograft of human gastric cancer in nude mice were examined 5-10 days after intravenous injection of a single dose of 211At labelled McAb(211At-3H11). The results showed that there was a relatively selective localization and therapeutic effect as biomissile, which led to a radiation response of gastric cancer cells (GCCs) and decreased the mitosis of GCCs with a dose-response correlation among the 8, 16, and 24 microCi 211At-3H11 groups. The effectiveness of a single dose of 211At-3H11 injected intravenously in the tumor bearing nude mice lasted not longer than 5-6 days, meanwhile in the mice exposed to the dosage of 16 or 24 microCi, mitosis in GCCs disappeared, and at the same time there was no obvious repair or regeneration of GCCs.

Topics: Animals; Antibodies, Monoclonal; Astatine; Female; Humans; Immunotoxins; Male; Mice; Mice, Inbred BALB C; Mice, Nude; Neoplasm Transplantation; Stomach Neoplasms

By means of nuclide precursor incorporation, the effects of 211At labelled monoclonal antibody against gastric cancer (211At-3H11McAb) on DNA, RNA and protein synthesis in gastric cancer cell were studied. The results show that 211At-3H11McAb and Na211At-3 inhibit 3H-TdR, 3H-UR and 3H-Leu incorporation, especially 3H-UR incorporation, into gastric cancer cell at 3.7 x 10(4)Bq and 1.85 x 10(5)Bq; the inhibiting rates depend on concentration. The DNA biosynthesis in gastric cancer cell gradually recover after the drug is removed, suggesting that the drug should exert an inhibiting action on DNA biosynthesis in tumor cell through interference of DNA metabolism.

Topics: Antibodies, Monoclonal; Astatine; DNA, Neoplasm; Humans; Immunotoxins; Neoplasm Proteins; RNA, Neoplasm; Stomach Neoplasms; Tumor Cells, Cultured