asialo-gm1-ganglioside and Leukemia--T-Cell

asialo-gm1-ganglioside has been researched along with Leukemia--T-Cell* in 2 studies

Other Studies

2 other study(ies) available for asialo-gm1-ganglioside and Leukemia--T-Cell

Article	Year
Successful graft of HTLV-I-transformed human T-cells (MT-2) in severe combined immunodeficiency mice treated with anti-asialo GM-1 antibody. Japanese journal of cancer research : Gann, 1992, Volume: 83, Issue:4 To develop an experimental model of adult T-cell leukemia/lymphoma in small animals, severe combined immunodeficiency (SCID) mice treated with anti-asialo GM-1 antibody were inoculated with MT-2 cells, a cell line transformed by the human T-cell leukemia virus (HTLV-I). Three mice injected with 4 x 10(7) cells subcutaneously or intramuscularly developed tumors at or near inoculation sites. Immunofluorescent antibody (IFA) staining for HTLV-I structural protein, p19, revealed the specific antigen in the cytoplasm of most cells from tumors and the DNA signals of HTLV-I proviral DNA were also positive in cellular DNA by polymerase chain reaction assay with HTLV-I tax gene primers, SK43/SK44. The MT-2 cells did not invade in mouse organs. Topics: Animals; Antibodies; Cell Line, Transformed; Cell Transformation, Viral; DNA, Neoplasm; Fluorescent Antibody Technique; G(M1) Ganglioside; Human T-lymphotropic virus 1; Humans; Leukemia, T-Cell; Lymphoma, T-Cell; Mice; Mice, SCID; Neoplasm Transplantation; Polymerase Chain Reaction; T-Lymphocytes; Transplantation, Heterologous	1992
Differential expression of the ASGM1 antigen on anti-reovirus and alloreactive cytotoxic T lymphocytes (CTL). Journal of immunogenetics, 1988, Volume: 15, Issue:4 Anti-reovirus cytotoxic effectors were found to be: (i) H-2 restricted; (ii) virus specific; (iii) non-lytic (in 4 h) for natural killer (NK)-sensitive YAC-1 cells; and (iv) positive for the Thy-1 and Lyt-2 lymphocyte markers. Thus, anti-reovirus cytotoxic effectors have the functional and phenotype characteristics of cytotoxic T lymphocyte (CTL). A significant fraction of anti-viral CTL, as well as alloreactive CTL, were also found to be positive for the asialo GM1 (ASGM1) cell surface antigen, generally considered to be a NK cell marker. ASGM1 expression on these CTL, as determined by sensitivity to antibody plus complement (C), appeared to be highly variable and dependent on two factors-the nature of the antigenic stimulus (viral vs. alloantigen), and the mouse strain from which the CTL originated. Thus, ASGM1 antigen expression on CTL appears to be regulated and may be under the control of lymphokines, development differentiation signals and/or other strain-dependent genetic factors. Topics: Animals; Antigens, Surface; Fibroblasts; G(M1) Ganglioside; Genetic Variation; Glycosphingolipids; H-2 Antigens; Isoantibodies; Killer Cells, Natural; Leukemia, T-Cell; Lymphocyte Depletion; Mice; Mice, Inbred Strains; Reoviridae; T-Lymphocytes, Cytotoxic; Thy-1 Antigens; Tumor Cells, Cultured	1988

Article

Year

Successful graft of HTLV-I-transformed human T-cells (MT-2) in severe combined immunodeficiency mice treated with anti-asialo GM-1 antibody.

Japanese journal of cancer research : Gann, 1992, Volume: 83, Issue:4

To develop an experimental model of adult T-cell leukemia/lymphoma in small animals, severe combined immunodeficiency (SCID) mice treated with anti-asialo GM-1 antibody were inoculated with MT-2 cells, a cell line transformed by the human T-cell leukemia virus (HTLV-I). Three mice injected with 4 x 10(7) cells subcutaneously or intramuscularly developed tumors at or near inoculation sites. Immunofluorescent antibody (IFA) staining for HTLV-I structural protein, p19, revealed the specific antigen in the cytoplasm of most cells from tumors and the DNA signals of HTLV-I proviral DNA were also positive in cellular DNA by polymerase chain reaction assay with HTLV-I tax gene primers, SK43/SK44. The MT-2 cells did not invade in mouse organs.

Topics: Animals; Antibodies; Cell Line, Transformed; Cell Transformation, Viral; DNA, Neoplasm; Fluorescent Antibody Technique; G(M1) Ganglioside; Human T-lymphotropic virus 1; Humans; Leukemia, T-Cell; Lymphoma, T-Cell; Mice; Mice, SCID; Neoplasm Transplantation; Polymerase Chain Reaction; T-Lymphocytes; Transplantation, Heterologous

1992

Differential expression of the ASGM1 antigen on anti-reovirus and alloreactive cytotoxic T lymphocytes (CTL).

Journal of immunogenetics, 1988, Volume: 15, Issue:4

Anti-reovirus cytotoxic effectors were found to be: (i) H-2 restricted; (ii) virus specific; (iii) non-lytic (in 4 h) for natural killer (NK)-sensitive YAC-1 cells; and (iv) positive for the Thy-1 and Lyt-2 lymphocyte markers. Thus, anti-reovirus cytotoxic effectors have the functional and phenotype characteristics of cytotoxic T lymphocyte (CTL). A significant fraction of anti-viral CTL, as well as alloreactive CTL, were also found to be positive for the asialo GM1 (ASGM1) cell surface antigen, generally considered to be a NK cell marker. ASGM1 expression on these CTL, as determined by sensitivity to antibody plus complement (C), appeared to be highly variable and dependent on two factors-the nature of the antigenic stimulus (viral vs. alloantigen), and the mouse strain from which the CTL originated. Thus, ASGM1 antigen expression on CTL appears to be regulated and may be under the control of lymphokines, development differentiation signals and/or other strain-dependent genetic factors.

Topics: Animals; Antigens, Surface; Fibroblasts; G(M1) Ganglioside; Genetic Variation; Glycosphingolipids; H-2 Antigens; Isoantibodies; Killer Cells, Natural; Leukemia, T-Cell; Lymphocyte Depletion; Mice; Mice, Inbred Strains; Reoviridae; T-Lymphocytes, Cytotoxic; Thy-1 Antigens; Tumor Cells, Cultured

1988