asialo-gm1-ganglioside and Chlamydia-Infections

asialo-gm1-ganglioside has been researched along with Chlamydia-Infections* in 2 studies

Other Studies

2 other study(ies) available for asialo-gm1-ganglioside and Chlamydia-Infections

ArticleYear
Regulation and role of IFN-gamma in the innate resistance to infection with Chlamydia pneumoniae.
    Journal of immunology (Baltimore, Md. : 1950), 2000, May-01, Volume: 164, Issue:9

    By using mice genomically lacking IFN-gammaR, IL-12, perforin, and recombination-activating gene-1 (RAG-1), we analyzed the regulation and importance of IFN-gamma in the control of infection with Chlamydia pneumoniae. IL-12 participates in resistance of mice to C. pneumoniae, probably by regulating the protective levels of IFN-gamma mRNA. In turn, IFN-gamma is necessary for the increased IL-12p40 mRNA accumulation that occurs in lungs during infection with C. pneumoniae, suggesting a positive feedback regulation between these two cytokines. In experiments including RAG-1-/-/IFN-gammaR-/- mice we showed that IFN-gamma produced by innate cells controls the bacterial load and is necessary for the increased accumulation of transcripts for enzymes controlling high output NO release (inducible NO synthase), superoxide production (gp-91 NADPH oxidase), and catalysis of tryptophan (indoleamine 2, 3-dioxygenase (IDO)), mechanisms probably related to bacterial killing. Adaptive immune responses diminish the levels of IFN-gamma and IL-12 mRNA and thereby the levels of inducible NO synthase, IDO, and gp91 NADPH oxidase transcripts. By using RAG-1-/-/perforin-/- mice, we excluded the overt participation of NK cell cytotoxicity in the control of C. pneumoniae. However, NK cells and probably other innate immune cells release IFN-gamma during the bacterial infection.

    Topics: Animals; Chlamydia Infections; Chlamydophila pneumoniae; Cytotoxicity, Immunologic; G(M1) Ganglioside; Genes, RAG-1; Genetic Predisposition to Disease; Immune Sera; Immunity, Innate; Immunosuppressive Agents; Injections, Intravenous; Interferon gamma Receptor; Interferon-gamma; Killer Cells, Natural; Mice; Mice, Inbred C57BL; Mice, Knockout; Receptors, Interferon; Sequence Deletion

2000
Role of NK cells in early host response to chlamydial genital infection.
    Infection and immunity, 1998, Volume: 66, Issue:12

    The cell-mediated immune response has been documented to be the major protective immune mechanism in mice infected genitally with the agent of mouse pneumonitis (MoPn), a biovar of Chlamydia trachomatis. Moreover, there is strong evidence to indicate that gamma interferon (IFN-gamma) is a major effector mechanism of the cell-mediated immune response. Previous studies from this laboratory have also reported that the dominant cell population in the genital tract is the CD4 Th1 population. When experiments were performed by the enzyme-linked immunospot assay, high numbers of cells producing IFN-gamma were found in the genital tract, concomitant with resolution of the infection; however, in addition, an increase in IFN-gamma-producing cells which were CD4(-) was seen early in the infection. Since natural killer (NK) cells produce IFN-gamma and have been found to participate in the early responses in other infections, we hypothesized that NK cells are responsible for early IFN-gamma production in the murine chlamydial model. NK cells were quantified by the standard YAC-1 cytotoxicity assay and were found to appear in the genital tract as early as 12 h after intravaginal infection with MoPn. The cells were confirmed to be NK cells by abrogation of YAC-1 cell cytotoxicity by treatment in vitro and in vivo with anti-asialo-GM1. The early IFN-gamma response could also be depleted by treatment with anti-asialo-GM1, indicating that NK cells were responsible for the production of this cytokine. Of interest was our observation that depletion of NK cells also exacerbated the course of infection in the mice and elicited a Th2 response, as indicated by a marked increase in immunoglobulin G1 antibody. Thus, these data demonstrate that NK cells are not only responsible for the production of IFN-gamma early in the course of chlamydial genital tract infection but are also, via IFN-gamma, a significant factor in the development of the Th1 CD4 response and in the control of the infection.

    Topics: Animals; Chlamydia Infections; Chlamydia trachomatis; Cytokines; Female; G(M1) Ganglioside; Genitalia, Female; Ilium; Immunoglobulin G; Immunoglobulin Isotypes; Interferon-gamma; Killer Cells, Natural; Lymph Nodes; Lymphocyte Depletion; Mice; Mice, Inbred BALB C; Pneumonia; Rodent Diseases; Th1 Cells; Vaginal Diseases

1998