asialo-gm1-ganglioside and Alzheimer-Disease

Topics: Alzheimer Disease; Biomarkers; G(M1) Ganglioside; Guillain-Barre Syndrome; Hematologic Tests; Humans; Motor Neuron Disease; Precursor Cell Lymphoblastic Leukemia-Lymphoma; Specimen Handling

Amyloid peptide (Abeta) is the major protein constituent of neuritic plaques in Alzheimer's disease (AD). This peptide is an amphipathic molecule that perturbs membranes and binds to raft-like membranes composed of gangliosides. Ganglioside GM1 binds tightly with Abeta and it is speculated that GM1 inhibits Abeta from undergoing alpha-helix to beta-sheet conformational changes. Although the role of gangliosides in conformational changes of Abeta have been studied, the specific nature of these interactions have not been reported. In the present report multidimensional NMR studies of ganglioside-Abeta interactions were conducted in sodium dodecyl sulphate (SDS) micelles, a membrane-mimicking environment. These studies reveal that asialoGM1 binds specifically with Abeta in a manner which could prevent beta-sheet formation. but that ganglioside GT1b does not bind Abeta. Plausible pathways for the involvement of gangliosides in amyloidogenesis are discussed.

Topics: Alzheimer Disease; Amyloid beta-Peptides; G(M1) Ganglioside; Gangliosides; Humans; Magnetic Resonance Spectroscopy; Micelles; Peptide Fragments; Sodium Dodecyl Sulfate

Topics: Alzheimer Disease; Animals; Antibodies; Brain; G(M1) Ganglioside; Glycosphingolipids; Humans; Lymphocyte Activation; Lymphocytes; T-Lymphocytes