ascorbic-acid and Uveitis--Anterior

Topics: Animals; Anterior Chamber; Aqueous Humor; Ascorbic Acid; Blood Pressure; Cataract; Child; Cryosurgery; Diabetes Complications; Glaucoma; Gonioscopy; Humans; Intraocular Pressure; Microsurgery; Miotics; Ophthalmodynamometry; Sclera; Thrombosis; Tonometry, Ocular; Uveitis, Anterior; Visual Field Tests

To investigate the effect of additional oral vitamins C and E on acute anterior uveitis.. A placebo controlled double masked study on the effect of vitamin C 500 mg in combination with vitamin E 100 mg twice daily in 145 patients with acute anterior uveitis. As a primary end point variable, laser cell/flare measurements were performed. Best corrected and stenopeic visual acuity (VA) testing and clinical variable scores were measured.. Laser flare measurements (ph/s) before treatment were 207.1 (SD 258) in the vitamin group and 143.6 (156) in the placebo group. After 3 days corresponding values were 80.2 (129) and 54.7 (82), after 7 days 89.2 (187) (12.5) and 85.8 (208), after 14 days 47.1 (109.5) and 40.5 (116) after 28 days 23.1 (53.6) and 23.1 (48), and after 56 days 15.6 (26) and 15.3 (17). There was no significant difference in time trend between the two treatment groups (RMANOVA; p = 0.53). Baseline VA (logMAR) was 0.106 (0.241) in the vitamin group and 0.128 (0.456) in the placebo group. VA after 3 days was 0. 236 (0.293) and 0.344 ( 0.489), after 7 days 0.204 (0.292) and 0.292 (0.479), after 14 days 0.162 (0.274) and 0.193 (0.454), after 28 days 0.096 (0.232) and 0.158 (0.436), and 0.026 (0.213) and 0.106 (0. 437) after 56 days. Although no significant difference in time trend was detected, evaluation of the VA data of the last time point (56 days) by means of the Mann-Whitney test showed a significantly better VA in the vitamin group (p = 0.01).. There was no significant effect of vitamins C and E on laser flare measurements. The significant effect of the oral vitamins on visual acuity at 8 weeks after start of the oral vitamins C and E may indicate a protective effect in patients with acute anterior uveitis.

Topics: Acute Disease; Administration, Oral; Adult; Aged; Analysis of Variance; Ascorbic Acid; Double-Blind Method; Drug Administration Schedule; Female; Humans; Male; Middle Aged; Uveitis, Anterior; Visual Acuity; Vitamin E

Human leukocyte antigen-B27 (HLA-B27)-positive acute anterior uveitis (AAU) has a higher recurrence rate and shows more anterior chamber cell infiltration compared with HLA-B27-negative patients, suggesting distinct etiologies of these clinically overlapping conditions. To advance our understanding of the biology of AAU, we characterized the metabolic profile of aqueous humor (AqH) of patients with HLA-B27-associated AAU (B27-AAU) and noninfectious idiopathic AAU (idiopathic AAU).. Experimental laboratory study.. AqH samples from 2 independent cohorts totaling 30 patients with B27-AAU, 16 patients with idiopathic AAU, and 20 patients with cataracts underwent 2 individual rounds of direct infusion mass spectrometry. Features predicted by direct infusion mass spectrometry that facilitated maximum separation between the disease groups in regression models were validated by liquid chromatography/tandem mass spectrometry-based quantification with appropriate standards.. Partial least square-discriminant analysis revealed metabolite profiles that were able to separate patients with B27-AAU from those with iodiopathic AAU. Pathway enrichment analysis, based on metabolites on which separation of the groups in the partial least square-discriminant analysis model was based, demonstrated the involvement of branched-chain amino acid biosynthesis, ascorbate and aldarate metabolism, the tricarboxylic acid cycle, and glycolysis-diverting pathways (eg, serine biosynthesis) across all investigated cohorts. Notably, the metabolite ketoleucine was elevated in B27-AAU across all 3 runs and moderately-but robustly-correlated with anterior chamber cell count (correlation coefficient range 0.41-0.81).. These results illustrate metabolic heterogeneity between HLA-B27-positive and HLA-B27-negative AAU, including an increase of branched-chain amino acid biosynthesis, that reflects disease activity in AAU.

Topics: Acute Disease; Adult; Aged; Amino Acids, Branched-Chain; Aqueous Humor; Ascorbic Acid; Biomarkers; Chromatography, Liquid; Citric Acid Cycle; Female; HLA-B27 Antigen; Humans; Keto Acids; Male; Middle Aged; Tandem Mass Spectrometry; Uveitis, Anterior

Topics: Aqueous Humor; Ascorbic Acid; Diabetes Mellitus; Electrophoresis, Capillary; Humans; Reference Values; Reproducibility of Results; Uric Acid; Uveitis, Anterior

The effect of topical administration of 3-isobutyl-methyl-xanthine (IBMX), a potent phosphodiesterase inhibitor, was studied on an experimentally provoked uveitis in rabbits. After presensitization with an intravitreal injection of human serum albumin (HSA), intravenous antigenic challenge induces blood-aqueous barrier breakdown and leukocyte infiltration. The effect of IBMX on the blood-aqueous barrier was determined by scoring the severity of the flare in the anterior chamber and by determination of the levels of ascorbic acid and protein in the aqueous. Treatment with IBMX 1% two times daily, significantly inhibited the breakdown of the blood-aqueous barrier and the increase in PGE2 level of the aqueous humor. There was no effect on leukocyte infiltration. The therapeutic effect of IBMX in blood-aqueous barrier protection is comparable with the effect of topical treatment with the corticosteroid medrysone.

Topics: 1-Methyl-3-isobutylxanthine; Animals; Aqueous Humor; Ascorbic Acid; Dinoprostone; Eye Proteins; Immune System Diseases; Leukocyte Count; Pregnenediones; Prostaglandins E; Rabbits; Serum Albumin; Theophylline; Uveitis, Anterior

Topics: Ascorbic Acid; Echothiophate Iodide; Epinephrine; Glaucoma; Humans; Iris; Pilocarpine; Propranolol; Quaternary Ammonium Compounds; Uveitis, Anterior