ascorbic-acid and Tobacco-Use-Disorder

Tobacco consumption is one of the leading causes of oral submucous fibrosis, oral cancer and even premature death. The present study was designed to compare the biochemical parameters and non- enzymatic antioxidant status and the lipid peroxidation products in pan masala tobacco users as compared with age-matched non-user controls.. Pan masala and tobacco users of age 33.2±9.94 years and age-matched controls (31.2±4.73 years) were enrolled for the study. Plasma levels of vitamin E, vitamin C, albumin, bilirubin, uric acid, glucose, urea, creatinine, aspartate amino transferase (AST), alanine amino transferase (ALT) were measured by standard methods. Serum malondialdehyde (MDA) levels were estimated as a measure of lipid peroxidation.. In the pan masala tobacco users, as compared to the controls, the level of vitamin C (68.5±5.9 vs 97.9±9.03 μmol/L, p≤0.05) vitamin E (18.4±5.3 vs 97.9±9.03 μmol/L, p≤0.001), albumin (37.5±7.01 vs 44.3±9.99 g/L, p≤0.001), and malondialdehyde (10.8±1.29 vs 1.72±1.15 nmol/ml, p≤0.001 ) were found to be significantly altered. Malondialdehyde was significantly correlated with vitamin E (r=1.00, p<0.001) and vitamin C (r =1.00, p<0.001) in pan masala tobacco users. Serum levels of AST (31.0±16.77 IU) and ALT (36.7±31.3 IU) in the pan masala tobacco users were significantly raised as compared to the controls (AST, 25.2±9.51 IU, p=0.038; ALT, 26.2±17.9 IU, p=0.038).. These findings suggest that pan masala tobacco users are in a state of oxidative stress promoting cellular damage. Non-enzymatic antioxidants are depleted in pan masala tobacco users with subsequent alteration in the biochemical parameters. Supplementation of antioxidants may prevent oxidative damage in pan masala tobacco users.

Topics: Adult; Alanine Transaminase; Antioxidants; Areca; Ascorbic Acid; Aspartate Aminotransferases; Bilirubin; Female; Humans; Male; Malondialdehyde; Nepal; Oxidative Stress; Serum Albumin; Tobacco Use Disorder; Tobacco, Smokeless; Vitamin E; Young Adult

Levels of plasma vitamin C and E (P-VC, P-VE), beta-carotene (P-beta-Car), lipoperoxides (P-LPO), and activity of erythrocyte superoxide dismutase (E-SOD) were measured in 871 healthy smokers and 348 healthy non-smokers to study the relationship between cigarette smoking and antioxidant vitamins and oxygen free radicals. Results showed that levels of P-VC, P-VE, P-beta-Car and E-SOD reduced significantly, and level of P-LPO increased significantly in smokers, as compared with those in non-smokers. Levels of P-VC, P-VE, P-beta-Car and E-SOD reduced and level of P-LPO increased in 65 male smokers at the same age, with the increase of duration of smoking and quantity of cigarettes smoked, showing a linear correlationship. It suggested that there existed serious imbalance between oxidation and antioxidation in the bodies of smokers, and oxygen free radical reaction and reaction of lipoperoxidation obviously intensified.

Topics: Adolescent; Adult; Ascorbic Acid; beta Carotene; Female; Humans; Lipid Peroxides; Male; Middle Aged; Smoking; Superoxide Dismutase; Tobacco Use Disorder; Vitamin E