ascorbic-acid and Squamous-Cell-Carcinoma-of-Head-and-Neck

Topics: Ascorbic Acid; Carcinoma, Squamous Cell; Cell Line, Tumor; Eating; Gene Expression Regulation, Neoplastic; Head and Neck Neoplasms; Humans; MicroRNAs; Squamous Cell Carcinoma of Head and Neck; Sugars; Vitamins

Over production of reactive oxygen species (ROS) caused by altered redox regulation of signaling pathways is common in many types of cancers. While PET imaging is recognized as the standard tool for cancer imaging, there are no clinically-approved PET radiotracers for ROS-imaging in cancer diagnosis and treatment. An ascorbate-based radio ligand promises to meet this urgent need. Our laboratory recently synthesized [

Topics: Animals; Ascorbic Acid; Doxorubicin; Head and Neck Neoplasms; Ligands; Liver Neoplasms; Male; Mice; Primates; Rats; Reactive Oxygen Species; Rodentia; Squamous Cell Carcinoma of Head and Neck; Tissue Distribution

A combination of metabolic modifications by light stimulus and photodynamic action is very attractive. Photobiomodulation therapy (PBMT) comprehends a vast range of applications and has been shown to be suitable to ease morbidities caused by chemotherapy and radiation, such as mucositis and dermatitis. The current study investigates the effects of near-infrared PBMT combined with porphyrin-based photodynamic therapy (PDT) in squamous cell carcinoma cell lines SCC-25 and SCC-4. The aim is to evaluate the potential of this combination to improve PDT outcome by increasing cell toxicity. Many techniques were used to verify the combined effect. Photobiomodulation (PBM) enhanced PDT action in SCC-25 cells by increasing photosensitizer (PS) uptake and production of reactive oxygen species (ROS). The equivalent was not seen in SCC-4 cells compared to the PDT only group. We believe these effects are strongly related to the interval of application between PBMT, PS incubation and PDT. Additionally, the effect of ascorbic acid on preventing PBM effects in PDT shows that ROS play an important role in the early mechanisms of PBM-PDT. Therefore, we believe PBM-PDT combination is worth exploring, for its benefit-cost ratio and simple protocols, along with the possibility of improvement in treatment resuts.

Topics: Antioxidants; Ascorbic Acid; Carcinoma, Squamous Cell; Cell Line, Tumor; Cell Proliferation; Cell Survival; Humans; Light; Photochemotherapy; Photosensitizing Agents; Reactive Oxygen Species; Squamous Cell Carcinoma of Head and Neck

The mainstay of the treatment of head and neck squamous cell carcinoma (HNSCC) is radiotherapy, which acts by producing free radicals. Therefore, this study was planned to observe the effect of radiotherapy on oxidative stress in patients of HNSCC.. This study was conducted on 50 histopathologically proven cases of HNSCC. The levels of nitric oxide, glutathione-S-transferase, and vitamin C were estimated colorimetrically before and after treatment in patients and in 30 age- and sex-matched healthy controls. The results were compared statistically.. The levels of nitric oxide and glutathione-S-transferase were significantly higher in patents as compared to controls and increased significantly after treatment. Vitamin C levels were significantly lower in patients as compared to controls and decreased significantly after treatment.. HNSCC leads to increased oxidative stress and treatment in the form of radiotherapy itself produces an accentuation of this stress.

Topics: Adult; Aged; Antioxidants; Ascorbic Acid; Carcinoma, Squamous Cell; Case-Control Studies; Female; Glutathione Transferase; Head and Neck Neoplasms; Humans; Male; Middle Aged; Neoplasm Staging; Nitric Oxide; Squamous Cell Carcinoma of Head and Neck; Young Adult