ascorbic-acid and Spondylitis--Ankylosing

Ankylosing spondylitis (AS) is a chronic inflammatory autoimmune disease, and the diagnosis and treatment of AS have been limited because its pathogenesis is still unclear. Pyroptosis is a proinflammatory type of cell death that plays an important role in the immune system. However, the relationship between pyroptosis genes and AS has never been elucidated.. GSE73754, GSE25101, and GSE221786 datasets were collected from the Gene Expression Omnibus (GEO) database. Differentially expressed pyroptosis-related genes (DE-PRGs) were identified by R software. Machine learning and PPI networks were used to screen key genes to construct a diagnostic model of AS. AS patients were clustered into different pyroptosis subtypes according to DE-PRGs using consensus cluster analysis and validated using principal component analysis (PCA). WGCNA was used for screening hub gene modules between two subtypes. Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were used for enrichment analysis to elucidate underlying mechanisms. The ESTIMATE and CIBERSORT algorithms were used to reveal immune signatures. The connectivity map (CMAP) database was used to predict potential drugs for the treatment of AS. Molecular docking was used to calculate the binding affinity between potential drugs and the hub gene.. Sixteen DE-PRGs were detected in AS compared to healthy controls, and some of these genes showed a significant correlation with immune cells such as neutrophils, CD8 + T cells, and resting NK cells. Enrichment analysis showed that DE-PRGs were mainly related to pyroptosis, IL-1β, and TNF signaling pathways. The key genes (TNF, NLRC4, and GZMB) screened by machine learning and the protein-protein interaction (PPI) network were used to establish the diagnostic model of AS. ROC analysis showed that the diagnostic model had good diagnostic properties in GSE73754 (AUC: 0.881), GSE25101 (AUC: 0.797), and GSE221786 (AUC: 0.713). Using 16 DE-PRGs, AS patients were divided into C1 and C2 subtypes, and these two subtypes showed significant differences in immune infiltration. A key gene module was identified from the two subtypes using WGCNA, and enrichment analysis suggested that the module was mainly related to immune function. Three potential drugs, including ascorbic acid, RO 90-7501, and celastrol, were selected based on CMAP analysis. Cytoscape showed GZMB as the highest-scoring hub gene. Finally, molecular docking results showed that GZMB and ascorbic acid formed three hydrogen bonds, including ARG-41, LYS-40, and HIS-57 (affinity: -5.3 kcal/mol). GZMB and RO-90-7501 formed one hydrogen bond, including CYS-136 (affinity: -8.8 kcal/mol). GZMB and celastrol formed three hydrogen bonds, including TYR-94, HIS-57, and LYS-40 (affinity: -9.4 kcal/mol).. Our research systematically analyzed the relationship between pyroptosis and AS. Pyroptosis may play an essential role in the immune microenvironment of AS. Our findings will contribute to a further understanding of the pathogenesis of AS.

Topics: Ascorbic Acid; Computational Biology; Humans; Molecular Docking Simulation; Pyroptosis; Spondylitis, Ankylosing

The aim of this study was to investigate plasma and erythrocytes antioxidant vitamins and lipid peroxidation (LP) levels in patients with ankylosing spondylitis (AS) and controls.. The study was performed on the blood of 13 AS patients and 13 controls. Plasma and erythrocyte samples were obtained from blood of the patients and controls.. Erythrocyte sedimentation ratio, C-reactive protein level, Bath AS disease activity index and bath AS functional index were higher in patients with AS than in controls. Vitamins A, C, E and β-carotene concentrations in plasma, reduced glutathione (GSH) and glutathione peroxidase values in erythrocyte were lower in patients with AS than in controls. LP concentrations were higher in the plasma and erythrocyte of the patients than in controls.. These results provide some evidence for a potential role of increased lipid peroxidation and decreased antioxidant vitamins in ankylosing spondylitis.

Topics: Adult; Antioxidants; Ascorbic Acid; beta Carotene; Biomarkers; Blood Sedimentation; C-Reactive Protein; Erythrocytes; Female; Glutathione; Glutathione Peroxidase; Humans; Lipid Peroxidation; Male; Malondialdehyde; Spondylitis, Ankylosing; Vitamin A; Vitamin E; Young Adult

To report the presence of an asymptomatic cilium in the subconjunctival area and an adjacent sterile scleral ulcer, together as a complication of subtenon injection of steroids, and the resolution of the ulcer despite the in situ cilium.. Medical management of a 51-year-old man, who was a known case of ankylosing spondylitis, with a 3-mm scleral ulcer located in the inferior palpebral area with one adjacent fully embedded cilium at the probable site of subtenon injection given for suspected acute iridocyclitis.. On altering the medication, the ulcer resolved completely. This occurred despite the in situ cilium, which did not act as a nidus for infection or inflammation.. The presence of cilia did not adversely affect the outcome of treatment of the adjacent ulcer. Therefore, if there is no exposure of the entrapped cilium through the conjunctiva, as was in this case, conservative management of the ulcer and not trying to remove the cilium seems to be a good alternative as opposed to reports in literature, which warrant its removal in most cases.

Topics: Anti-Bacterial Agents; Ascorbic Acid; Carboxymethylcellulose Sodium; Chloramphenicol; Cilia; Conjunctival Diseases; Drug Therapy, Combination; Eye Foreign Bodies; Humans; Iatrogenic Disease; Injections, Intraocular; Iridocyclitis; Male; Middle Aged; Scleral Diseases; Spondylitis, Ankylosing; Steroids; Tetracycline; Ulcer; Vitamins

A 38 years old man presented with 2 years history of low backache and progressively increasing stiffness of the spine. Movements were restricted at lumbar spine due to stiffness especially forward flexion was markedly reduced. He was suspected to be suffering from ankylosing spondylitis. There was no tenderness over sacroiliac joints or lumbar spine. Yellowish green Ochronotic pigmentation of cartilage of ears was noted. Radiographs of lumbar and thoracic spine revealed narrowing of inter-vertebral spaces with calcification of intervertebral discs. Homogentisic acid was present in the patient's urine sample, suggesting him to be suffering from Alkaptonuria. Patient is being managed with non steroidal anti-inflammatory drugs and vitamin C, 1g daily.

Topics: Adult; Alkaptonuria; Anti-Inflammatory Agents, Non-Steroidal; Ascorbic Acid; Homogentisic Acid; Humans; Intervertebral Disc; Low Back Pain; Male; Ochronosis; Radiography; Spondylitis, Ankylosing; Treatment Outcome; Vitamins

Topics: Ascorbic Acid; Famous Persons; History, 20th Century; Holistic Health; Humans; Male; Spondylitis, Ankylosing; United States

Topics: Arthritis, Rheumatoid; Ascorbic Acid; Blood Sedimentation; Cervical Vertebrae; Galvanic Skin Response; Humans; Male; Physical Fitness; Prostate; Shoulder Joint; Spondylitis, Ankylosing

Topics: Anticoagulants; Arthritis; Arthritis, Rheumatoid; Ascorbic Acid; Aspirin; Gout; Hip Joint; Humans; Joint Diseases; Rheumatology; Spondylitis; Spondylitis, Ankylosing

Topics: Antimalarials; Arthritis; Arthritis, Rheumatoid; Ascorbic Acid; Gout; Humans; Psoriasis; Spondylitis; Spondylitis, Ankylosing; Sunlight; Triamcinolone; Ultraviolet Rays; Vitamin A

Topics: Ankylosis; Ascorbic Acid; Blood Chemical Analysis; Humans; Spondylitis; Spondylitis, Ankylosing

Topics: Ankylosis; Ascorbic Acid; Balneology; Glutathione; Humans; Spondylitis; Spondylitis, Ankylosing; Vitamins