ascorbic-acid and Spermatic-Cord-Torsion

Testicular torsion is still an urgent surgical condition and without any treatment it can cause infertility. The main pathophysiology of testicular torsion ischaemic injury however; the main sequalae of detorsion is reperfusion injury. Furthermore; treatments to prevent ischemic reperfusion injury due to decreased blood flow are important to preserve testicular function.. Human chorionic gonadotropin β (β-hCG) is an anabolic hormone that supports steroidogenesis and spermatogenesis. Vitamin C (Vit-C) is one of the water-soluble vitamins and is also a potent antioxidant in ischemic damage. Moreover, it has protective effects by increasing blood and lymph flow in the testicles. The aim of this study is to investigate the effects of β-hCG, Vit-C and their combination on ischemic reperfusion injury occurring after surgical treatment of testicular torsion.. Animal research studies.. The study was performed on 25 male Wistar albino rats. The animals were divided equally into 5 groups. In the first group "Control Group," left orchiectomy was performed. In the second group "Sham Group," a 720° clockwise torsion was created and after 4 h of left testicular torsion it was detorsioned for 4 h and then left orchiectomy was performed. In the third group same procedure was applied with 30 mg vitamin C was administered via intraperitoneal route once a week for 3 weeks. In the fourth group after same surgical procedures 75 IU β-hCG was administered via intraperitoneal route once a week for 3 weeks. In the fifth group after 4 h left testicle torsion it was detorsioned for 4 h then, 75 IU β-hCG and vitamin C together were administered via intraperitoneal route once a week for 3 weeks. Left orchiectomy was performed after 3 weeks in the third, fourth and fifth groups. Specimens were evaluated histologically.. Testicular tissue histopathological evaluations were performed. A high histopathological stage indicates more testicular damage, and a low one was indicated less testicular damage. The average histopathological grade of vitamin C + β-hCG group was significantly higher than the average histopathological grade of the control, the sham group and vitamin C group. The average histopathological grade of the vitamin C group was significantly lower than the average histopathological grade of sham and β-hCG groups. The ratio of the testicular atrophy of the Vitamin C + β-hCG group (100%) was higher than sham (40%) and β-hCG (40%) groups with a significant difference. A significant statistical difference was found among all groups histopathological grades of testicular tissue.. In animals taking vitamin C, an improvement of histopathological findings and a significant decrease in histological stages has been provided. However, it was observed that the histological findings of β-hCG and β-hCG + vitamin C groups worsened. It was found that β-hCG increased oxidative damage in the testicles and this damage can be so severe that exceeding the capacity of potent antioxidants such as Vitamin C. We believe that β-hCG can be harmful to testicles exposed to oxidative damage.

Topics: Animals; Antioxidants; Ascorbic Acid; Humans; Ischemia; Male; Rats; Rats, Wistar; Reperfusion Injury; Spermatic Cord Torsion; Testicular Diseases; Testis; Vitamins

The aim was to investigate the protective effect of vitamin C on tissue damage and oxidative stress following tunica albuginea incision with tunica vaginalis flap coverage for testicular torsion.. Adult male Wistar rats were randomly divided into two experimental groups. The first group experienced 5h of testicular torsion followed by treatment with vitamin C alone, with tunica vaginalis flap coverage alone, and with both vitamin C and tunica vaginalis flap coverage along with a control group subjected to a sham procedure. The second group experienced 9h of testicular torsion followed by the same treatment options as described for the 5h group. The oxidative stress and testosterone levels were measured 24h posttreatment. The Johnsen score, diameter of the seminiferous tubules, and thickness of the seminiferous tubule epithelium were recorded 30days following the treatment.. The Johnsen score, diameter of the seminiferous tubules, and thickness of the seminiferous tubule epithelium significantly increased in the 5h testicular torsion group receiving treatment with vitamin C and tunica vaginalis flap coverage compared with the group receiving tunica vaginalis flap alone. The level of testosterone decreased significantly in all groups except for the 5h testicular torsion group receiving treatment with vitamin C and tunica vaginalis flap coverage. The MDA level also decreased in the group receiving treatment with vitamin C and tunica vaginalis flap coverage compared with the group receiving tunica vaginalis flap coverage alone.. The results showed that the histological parameters and testosterone levels improved with the administration of vitamin C before tunica vaginalis flap coverage in the group experiencing 5h of torsion. This may be a result of the antioxidant effect of vitamin C. No advantage was observed for the 9h group, possibly because the dosage of vitamin C was inadequate.

Topics: Animals; Antioxidants; Ascorbic Acid; Male; Oxidative Stress; Rats; Rats, Wistar; Seminiferous Tubules; Spermatic Cord Torsion; Surgical Flaps; Testis

The objective of this study was to investigate the effects of dopamine as vasodilator, vitamin C as an antioxidant and combined administration of them on ischaemia-reperfusion (I-R) injury following testicular torsion (TT). Thirty adult male rats were divided into six groups each containing five rats. Testicular ischaemia was achieved by twisting the left testis for 4 h. Group 1 was for determination of the basal values. Group 2 had 4 h TT. Group 3 had 4 h TT and was then treated with dopamine. Group 4 had 4 h TT and was then treated with vitamin C. Group 5 had 4 h TT and was then treated with dopamine and vitamin C. Group 6 was designed as a sham operated group. Testicular torsion caused a significant decrease in the percentage of spermatogenesis and seminiferous tubules diameters compared with the control and sham groups. Administration of dopamine, vitamin C and their combination increased above mentioned parameters and decreased serum malondialehyde levels significantly. However, vitamin C had better results than the other treatments (P < 0.05). In conclusion, a potent antioxidant like vitamin C was found to be more effective than increasing blood flow by a vasodilator like dopamine on improving I-R injury following TT.

Topics: Animals; Antioxidants; Ascorbic Acid; Dopamine; Drug Therapy, Combination; Male; Malondialdehyde; Rats; Rats, Sprague-Dawley; Reperfusion Injury; Seminiferous Tubules; Spermatic Cord Torsion; Spermatogenesis; Vasodilator Agents