ascorbic-acid and Skin-Diseases

Dermatologists play a critical role in diagnosing and managing nutritional deficiencies as they often present with cutaneous findings. Traditionally, nutritional dermatoses are taught in the context of developing countries, famine, population displacement, and poor health care access; however, in the United States, common risk factors include chronic liver disease, alcoholism, psychiatric disease, bariatric surgery, inflammatory bowel disease, and hemodialysis. Additionally, nutritional dermatoses may be underdiagnosed in the United States and result in increased morbidity and utilization of hospital resources. There is a need for providers in developed nations to identify these deficiencies, and this review aims to meet that practice gap and provide relevant context to these diseases for dermatologists. This 2-part review series will focus on the epidemiology, impact, appearance, and diagnostic modalities for micronutrient deficiencies, including zinc, selenium, copper, and vitamins A and C in part 1. The companion review will focus on the B-complex vitamins.

Topics: Ascorbic Acid; Copper; Humans; Malnutrition; Micronutrients; Selenium; Skin Diseases; Vitamin A; Vitamins; Zinc

Cosmeceuticals containing antioxidants are among the most popular antiaging remedies. Topically applied antioxidants exert their benefits by offering protection from damaging free radicals produced when skin is exposed to ultraviolet light or allowed to age naturally. Vitamin C is a naturally occurring potent water-soluble antioxidant. Accordingly, it has been incorporated into a variety of cosmeceuticals designed to protect and rejuvenate photoaged skin.. This article reviews the scientific data and clinical studies supporting the use of topically applied vitamin C for treating photoaged skin. Other innovative uses for vitamin C cosmeceuticals are also discussed.. A significant body of scientific research supports the use of cosmeceuticals containing vitamin C. Cutaneous benefits include promoting collagen synthesis, photoprotection from ultraviolet A and B, lightening hyperpigmentation, and improvement of a variety of inflammatory dermatoses. Because of the diverse biologic effects of this compound, topical vitamin C has become a useful part of the dermatologist's armamentarium.

Topics: Administration, Topical; Antioxidants; Ascorbic Acid; Dermatologic Agents; Humans; Skin Aging; Skin Diseases

Topics: Antioxidants; Ascorbic Acid; Humans; Skin Diseases

To review some of the promising natural remedies within dermatology to explore their potential clinical benefit in supplementing conventional drugs.. MEDLINE searches from January 1966 through October 2000 and Science Citation Index searches from January 1974 through October 2000 were conducted.. Primary importance was given to in vivo and in vitro controlled studies, the results of which encourage further exploration.. The controls used, the statistical approach to analysis, and the validity of the experimental method analyzed were considered particularly important. Data were independently extracted by multiple observers.. Natural remedies seem promising in treating a wide variety of dermatologic disorders, including inflammation, phototoxicity, psoriasis, atopic dermatitis, alopecia areata, and poison oak.. The alternative medications presented seem promising, although their true effects are unknown. Many of the presented studies do not allow deduction of clinical effects. Further experimentation must be performed to assess clinical benefit.

Topics: Ascorbic Acid; Complementary Therapies; Drugs, Investigational; Fatty Acids, Essential; Humans; Oils, Volatile; Phytotherapy; Plant Extracts; Salicylates; Skin Diseases; Tea; Vitamin E

Unlike most animals, which form ascorbic acid by metabolizing glucose, humans require an exogenous source. Vitamin C occurs primarily in fruits and vegetables, and scurvy develops from inadequate consumption of these sources, usually because of ignorance about proper nutrition, psychiatric disorders, alcoholism, or social isolation. The earliest symptom of scurvy, occurring only after many weeks of deficient intake, is fatigue. The most common cutaneous findings are follicular hyperkeratosis, perifollicular hemorrhages, ecchymoses, xerosis, leg edema, poor wound healing, and bent or coiled body hairs. Gum abnormalities, which occur only in patients with teeth, include gingival swelling, purplish discoloration, and hemorrhages. Pain in the back and joints is common, sometimes accompanied by obvious hemorrhage into the soft tissue and joints. Syncope and sudden death may occur. Anemia is frequent, leukopenia occasional. Treatment with vitamin C results in rapid, often dramatic, improvement. (J Am Acad Dermatol 1999;41:895-906.). At the conclusion of this learning activity, participants should be familiar with the history, pathogenesis, clinical features, and treatment of scurvy in adults.

Topics: Adult; Animals; Ascorbic Acid; Cardiovascular Diseases; History, 15th Century; History, 17th Century; History, 18th Century; History, 19th Century; History, 20th Century; Humans; Nutrition Policy; Respiratory Tract Diseases; Scurvy; Skin Diseases

Vitamins have been increasingly used as prophylactic and therapeutic agents in the management of skin disorders. The current literature is replete with studies that promote the potential benefits of these compounds and attempt to elucidate their mechanisms of action. We review the literature and discuss the roles, safety, and efficacy of vitamins A, C, and E and related compounds in cutaneous health and disease.

Topics: Ascorbic Acid; Dermatologic Agents; Humans; Skin Diseases; Vitamin A; Vitamin E; Vitamins

Sun exposure has been linked to several types of skin damage including sun burn, photoimmunosuppression, photoaging and photocarcinogenesis. In view of the increasing awareness of the potentially detrimental long term side effects of chronic solar irradiation there is a general need for safe and effective photoprotectants. One likely hypothesis for the genesis of skin pathologies due to solar radiation is the increased formation of reactive oxidants and impairment of the cutaneous antioxidant system. Consequently, oral antioxidants that scavenge reactive oxidants and modulate the cellular redox status may be useful; systemic photoprotection overcomes some of the problems associated with the topical use of sunscreens. Preclinical studies amply illustrate the photoprotective properties of supplemented antioxidants, particularly RRR-alpha-tocopherol, L-ascorbate and beta-carotene. However, clinical evidence that these antioxidants prevent, retard or slow down solar skin damage is not yet convincing. The purpose of this review is to provide the reader with current information on cutaneous pathophysiology of photoxidative stress, to review the literature on antioxidant photoprotection and to discuss the caveats of the photo-oxidative stress hypothesis.

Topics: Antioxidants; Ascorbic Acid; beta Carotene; Humans; Oxidative Stress; Signal Transduction; Skin Diseases; Sunlight; Ultraviolet Rays; Vitamin E

Topics: Ascorbic Acid; Ascorbic Acid Deficiency; Drug Evaluation; Drug Therapy, Combination; Humans; Skin Diseases

Topics: Adult; Aged; Ascorbic Acid; Drug Eruptions; Drug Hypersensitivity; Eczema; Erythema Nodosum; Exanthema; Female; Humans; Lymphocyte Activation; Male; Medical History Taking; Middle Aged; Occupational Diseases; Penicillins; Photosensitivity Disorders; Pyridoxine; Radioallergosorbent Test; Skin Diseases; Stevens-Johnson Syndrome; Sulfonamides; Surveys and Questionnaires

It is apparent that significant progress has been made in our understanding of the biosynthesis, modifications, and maturation of collagen and elastin. We now recognize and partially understand special reactions involved in hydroxylations within the cell and complex cross-linking processes occurring outside the cell. Recent experiments (191) have shown that in human diploid fibroblast cultures of limited doubling potential (191) the hydroxylation of collagen prolyl residues appears to be "age" or passage-level dependent. With increasing passage level of these cultures, both the ascorbate requirements and the extent of collagen hydroxylation decrease. "Young" cell cultures have a strong requirement for complete hydroxylation and without ascorbate there is only about 50% of the normal level. "Middle-aged" cultures show higher hydroxylation without and full hydroxylation with ascorbate, whereas "old" (or cultures close to "senescence") are incapable of full hydroxylation with or without ascorbic acid. Although the overall system may show some deterioration with increasing passage levels, it appears that with increasing passage levels other components in the cell replace the ascorbate dependence of the hydroxylase system to a greater exten. In some ways, aging WI-38 cultures begin to resemble some transformed cells in their biochemical reactions, although they continue to remain diploid and eventually lose the ability to replicate. It is not yet known whether old animals can produce collagen, which may now be underhydroxylated, perhaps contributing to certain senescent changes. Careful examination of the hydroxylation index of collagen produced in organoid cultures of tissue biopsies as a function of donor age might be informative, particularly if one looks at the quality of collagen by employing collagenase and other proteolytic digests with collagen (191). One could comare the levels of frequent and characteristic peptide triplet sequences such as Gly-Pro-Hyp to Gly-Pro-Pro, Gly-Ala-Hyp to Gly-Ala-Pro, or Gly-Pro-Hyl to Gly-Pro-Lys and others for evaluation of hydroxylation throughout the entire molecule or at selected sequences.

Topics: Amino Acid Sequence; Animals; Antibody Specificity; Ascorbic Acid; Collagen; Connective Tissue; Copper; Ehlers-Danlos Syndrome; Elastin; Epitopes; Homocystinuria; Humans; Hydralazine; Lathyrism; Marfan Syndrome; Molecular Conformation; Platelet Aggregation; Procollagen-Proline Dioxygenase; Skin Diseases; Syndrome

Topics: Adenocarcinoma, Scirrhous; Adult; Amino Acids; Animals; Ascorbic Acid; Cattle; Child; Collagen; Collagen Diseases; Female; Humans; Hydroxyproline; Lung; Marfan Syndrome; Mice; Procollagen-Proline Dioxygenase; Skin Diseases; Stomach Neoplasms

Topics: Adult; Age Factors; Anemia; Animals; Ascorbic Acid; Ascorbic Acid Deficiency; Bone Diseases, Developmental; Capillaries; Child; Collagen Diseases; Connective Tissue; Creatinine; Dentin; Gingival Hemorrhage; Hemorrhage; Humans; Hydroxyproline; Infant; Scurvy; Skin Diseases; Species Specificity

Tetra-isopalmitoyl ascorbic acid (VC-IP), an oil-soluble vitamin C derivative, is known to be effective for treating photoaging skin caused by oxidative stress. In addition, it is known that morphological changes of the skin occur on the face with aging, and the main cause has been reported to be photoaging.. There have not been any published reports that examined the clinical effects of vitamin C derivatives on periorbital wrinkles. The purpose of this study was to characterize the potential anti-wrinkle effect of VC-IP.. A double-blind, randomized, placebo-controlled, split face clinical trial was performed on 3 groups of 23 female subjects each who used 1%, 2%, or 3% VC-IP creams versus a placebo cream to treat their facial wrinkles. VC-IP cream was applied on the periorbital area on one side of the face of each subject twice a day for 8 weeks and a placebo cream was applied on the other side. Anti-wrinkle effects were evaluated by visual wrinkle grading and topographic assessments.. Periorbital wrinkles treated with VC-IP twice a day for 8 weeks showed marked or moderate improvements according to visual observations and grading of photographs. Moreover, topographic assessments confirmed the improving effects of VC-IP, and more interestingly, the appearance of those effects differed depending on the VC-IP concentration. In addition, sufficient improvement was observed in parameters reported to be associated with dermal structure at lower concentrations of VC-IP.. These results indicate that topical application of VC-IP cream reduces periorbital wrinkles caused by skin aging.

Topics: Ascorbic Acid; Emollients; Female; Glycolipids; Humans; Skin; Skin Aging; Skin Diseases; Treatment Outcome; Vitamins

A powdered teat dip designed for winter usage was evaluated for bacteriological efficacy and teat conditioning qualities. A positive control, natural exposure field trial was conducted for 3 mo on 509 lactating cows. Two sets of cows, primiparous and multiparous, were used. The trial compared efficacy of a powdered teat dip with a teat dip of 1% iodine plus 10% glycerin. Bacteriological efficacy among primiparous cows was equivalent for all major mastitis pathogens, environmental pathogens, and streptococci other than Streptococcus agalactiae. Efficacy was not equivalent against coagulase-negative staphylococci and all mastitis pathogens. Results suggested that the positive control product was more efficacious. Among multiparous cows, efficacy was equivalent against environmental mastitis pathogens and bacteriologically negative, clinical mastitis. The products were not equivalent against Staphylococcus aureus, coagulase-negative staphylococci, or all major mastitis pathogens, once again suggesting that the positive control product was more efficacious. Data indicated that germicidal activity of the powdered dip was not sufficient to reduce the incidence of new IMI caused by contagious or minor pathogens normally associated with teat skin. Application of a powdered postmilking teat dip during 3 winter mo in Idaho resulted in improved teat end condition among primiparous and multiparous dairy cows. Teat skin condition improved among primiparous but not among multiparous cows.

Topics: Allantoin; Animals; Ascorbic Acid; Cattle; Disinfection; Female; Glycerol; Iodine; Mammary Glands, Animal; Mastitis, Bovine; Milk; Parity; Powders; Seasons; Skin Diseases; Staphylococcus aureus; Streptococcus

Topics: Adult; Ascorbic Acid; Carbon Isotopes; Clinical Trials as Topic; Diet; Electrocardiography; Electroencephalography; Enteral Nutrition; Eye Diseases; Feces; Glucose Tolerance Test; Hematology; Hemorrhage; Humans; Keratosis; Male; Nutritional Physiological Phenomena; Nutritional Requirements; Oral Hemorrhage; Scurvy; Skin Diseases; Urine

Atopic dermatitis (AD) is a chronic inflammatory disease characterized by dry and itchy skin. Recently, it has been reported that oxidative stress is involved in skin diseases, possibly including AD. Vitamin C, also referred to as ascorbic acid, is a vital water-soluble compound that functions as an essential nutrient. It plays a significant role as both an antioxidant and an additive in various pharmaceutical and food products. Despite the fact that vitamin C is easily oxidized, we have developed NXP081, a single-stranded DNA aptamer that selectively binds to vitamin C, thereby inhibiting its oxidation. The objective of the current research was to examine the impact of NXP081, an animal model of AD induced by 2,4-dinitrofluorobenzene (DNFB). The experimental drug NXP081, when taken orally, showed promising results in reducing inflammation and improving the skin conditions caused by DNFB. The administration of NXP081 resulted in a significant reduction in ear swelling and a noticeable improvement in the appearance of skin lesions. In addition, the administration of NXP081 resulted in a significant decrease in the migration of mast cells in the skin lesions induced by DNFB. Moreover, NXP081 inhibited the production of interferon-gamma (IFN-γ) in CD4

Topics: Animals; Aptamers, Nucleotide; Ascorbic Acid; CD4-Positive T-Lymphocytes; Cytokines; Dermatitis, Atopic; Dinitrofluorobenzene; Mice; Mice, Inbred BALB C; Skin; Skin Diseases; Vitamins

An 18-year-old man presented to our hospital with muscular pain, diffuse petechiae, spontaneous thigh ecchymosis, edema and pain of the right knee, bilateral pretibial subcutaneous nodules, and gingival hypertrophy and hemorrhage. His history was positive for a mixed anxiety-depressive disorder and a restrictive diet caused by self-diagnosed food allergies. Skin lesions appeared like hyperkeratotic papules with coiled hairs and perifollicular hemorrhages. A diagnosis of scurvy was made upon demonstration of low serum levels of ascorbic acid. An allergy evaluation found cross-reactivity between pollens and food, related to the presence of panallergens. Moreover, we found that our patient was also affected by celiac disease. In conclusion, scurvy should be considered in the differential diagnosis of patients with petechiae and ecchymosis, especially when food restriction, malabsorption, or psychiatric disorders are present.

Topics: Adolescent; Ascorbic Acid; Diagnosis, Differential; Humans; Male; Scurvy; Skin Diseases

Particulate matter is suspected to be substantially involved in pollution-induced health concerns. In fact, ultrafine particles (UFPs) contain polycyclic aromatic hydrocarbons (PAHs) known as mutagenic, cytotoxic and sometimes phototoxic. Since UFPs reach blood circulation from lung alveoli, deep skin is very likely contaminated by PAHs coming from either skin surface or blood. As photoreactive, benzo(a)pyrene (BaP) or indenopyrene (IcdP) is involved in the interplay between pollution and sunlight. In order to better characterize this process, experiments were carried out on reconstructed human epidermis (RHE) in a protocol mimicking realistic exposure. Concentrations of PAHs comparable to those generally reported in blood were used together with chronic irradiation to low dose UVA1. On a histological level, damaged cells mainly accumulated in a suprabasal situation, thus reducing living epidermis thickness. Stress markers such as IL1-α or MMP3 secretion increased, and surprisingly, the histological position of Transglutaminase-1 within epidermis was disturbed, whereas position of other differentiation markers (keratin-10, filaggrin, loricrin) remained unchanged. When vitamin C was added in culture medium, a very significant protection involving all markers was noticed. In conclusion, we provide here a model of interest to understand the epidermal deleterious consequences of pollution and to select efficient protective compounds.

Topics: Ascorbic Acid; Epidermis; Humans; Particulate Matter; Polycyclic Aromatic Hydrocarbons; Skin Diseases; Ultraviolet Rays; Vitamins

Skin sensitivity is a serious problem for many people, and it can be induced by various factors such as UV irradiation, physical and mental stresses, air pollution, dry air and so on. Skin dryness triggered by UV and dry air is one of the most important causes inducing the development of sensitive skin, and it has been reported that oxidative stress contributes to skin dryness. In this study, we investigated whether treatment with 3-O-laurylglyceryl ascorbate (VC-3LG), which is an amphipathic ascorbic acid derivative, can suppress the development of sensitive skin. The results demonstrate that VC-3LG restores the expression levels of interleukin-1α, nerve growth factor and matrix metalloprotease-9 in the dry skin models of reconstructed human epidermal equivalents (RHEEs) and in H

Topics: Air; Animals; Ascorbic Acid; Dendrites; Epidermis; Humans; Hydrogen Peroxide; Interleukin-1alpha; Keratinocytes; Laurates; Oxidative Stress; PC12 Cells; Rats; Skin; Skin Diseases; Ultraviolet Rays

Topics: Animals; Ascorbic Acid; Ascorbic Acid Deficiency; Atrophy; Humans; Hyaluronan Receptors; Hyaluronic Acid; Mice; Signal Transduction; Skin; Skin Aging; Skin Diseases; Vitamins

The incidence of accidental and intentional acid skin burns is rising. Current treatment strategies are mostly inadequate, leaving victims disfigured and without treatment options. Here, we have shown that transplantation of adipose-derived stem cells (ASCs) accelerates the process of acid burn wound-healing. Pre-conditioning of ASCs using ascorbic acid (AA) or hypoxic conditions provided additional benefit. While the wounds were ultimately healed in all mice, histological analysis revealed that, in non-transplanted animals, the number of hair follicles was reduced. Bioluminescent imaging (BLI) of transplanted ASCs revealed a gradual loss of transplanted cells, with a similar rate of cell death for each treatment group. The signal of fluorinated cells detected by a clinically applicable

Topics: Acids; Adipocytes; Animals; Ascorbic Acid; Burns, Chemical; Cell Hypoxia; Cell Survival; Cells, Cultured; Disease Models, Animal; Fluorine-19 Magnetic Resonance Imaging; Humans; Luminescent Measurements; Male; Mice; Multimodal Imaging; Skin Diseases; Stem Cell Transplantation; Treatment Outcome; Wound Healing

Topics: Aged, 80 and over; Ascorbic Acid; Fatal Outcome; Humans; Male; Malnutrition; Scurvy; Skin Diseases

The use of complementary and alternative medicine (CAM) among patients with dermatologic conditions has not been well studied. The aim of this study was to evaluate the frequency and pattern of CAM use in patients referred to the dermatology department of a tertiary care center. Patients referred to the dermatology department of an academic tertiary referral center between February 2, 2010, and February 10, 2010, were invited to participate in an 86-question survey regarding CAM use during the previous year. A total of 300 patients completed the survey, of whom 154 (51%) were women. Eighty-two percent (n = 247) of the respondents had used some type of CAM during the previous year. The most frequently used treatment and technique was massage therapy (33%), and the most commonly used vitamin was vitamin C (31%). Herbs or other dietary supplements were used by 58% (n = 173) of patients. Seventy-eight percent (n = 235) of patients stated that physicians should consider incorporating CAM approaches into their treatment recommendations, and 89% of patients (n = 267) stated that our dermatology department should study CAM approaches in research studies. CAM utilization is high among patients at a large academic dermatology department. Patients indicated a strong preference for having CAM approaches incorporated into their treatment recommendations and believed in the value of clinical studies to further refine the role of CAM.

Topics: Adult; Ascorbic Acid; Attitude to Health; Complementary Therapies; Dermatology; Dietary Supplements; Female; Health Care Surveys; Hospitals; Humans; Integrative Medicine; Male; Massage; Middle Aged; Phytotherapy; Plant Extracts; Referral and Consultation; Skin Diseases; Vitamins; Young Adult

The effects of oral administration of Musa sapientum and Musa suerier on prevention of UVB induced skin damages were investigated in male ICR mice. Animals were orally administered 50mg/day ascorbic acid, or M. sapientum or M. suerier's fruit pulps at dose of 0.5, 1 or 1.5 mg/g body weight/day for 12 weeks. Concurrently, the shaved backs of animals were irradiated with UVB for 12weeks. The intensity of irradiation was progressively increased, from 54 mJ/cm(2) per exposure at week 1-126 mJ/cm(2) at week 11. A significant decrease (p<0.05) in skin elasticity (from 0.82±0.02 to 0.42±0.09) and total glutathione (from (193.6±18.7 to 152.7±7.8 ng/mg protein) as compared with the control group (water-administered UVB-irradiated mice) was observed after 12 weeks of UVB exposure. When L-ascorbic acid (0.72±0.01) or 1mg/g body weight/day M. suerier (0.84±0.06) were administered to UVB-irradiated mice, the reduction in skin elasticity was significantly inhibited (p<0.05). Moreover, the significant increase (p<0.05) in level of total glutathione was found in these groups (220.8±13.3 ng/mg protein for l-ascorbic acid and 224.9±20.1 ng/mg protein for M. suerier). These findings suggest the potential effect of daily consumption of M. suerier on prevention of skin damage from repeated UVB exposure.

Topics: Administration, Oral; Animals; Ascorbic Acid; Free Radical Scavengers; Glutathione; Immunohistochemistry; Male; Mice; Mice, Inbred ICR; Musa; Phenols; Plant Extracts; Skin; Skin Diseases; Transforming Growth Factor beta1; Ultraviolet Rays

Frequent or rare, minor or serious, numerous skin conditions exist. Allergic, inflammatory, infectious or bullous, dermatoses may also reveal underlying pathologies.

Topics: Acne Vulgaris; Adrenal Cortex Hormones; Ascorbic Acid; Ascorbic Acid Deficiency; Dermatomyositis; Erythema; Humans; Lupus Erythematosus, Cutaneous; Psoriasis; Skin Diseases; Skin Diseases, Vesiculobullous; Vitamin A; Vitamin A Deficiency

An estimated 25-40 million of the 127 million people of Bangladesh have been exposed to high levels of naturally occurring arsenic from drinking groundwater. The mitigating effects of diet on arsenic-related premalignant skin lesions are largely unknown.. The purpose of this study was to clarify the effects of the vitamin B group (thiamin, riboflavin, niacin, pyridoxine, and cobalamin) and antioxidants (vitamins A, C, and E) on arsenic-related skin lesions.. We performed a cross-sectional study using baseline data from the Health Effects of Arsenic Longitudinal Study (HEALS), 2000-2002, with individual-level, time-weighted measures of arsenic exposure from drinking water. A total of 14,828 individuals meeting a set of eligibility criteria were identified among 65,876 users of all 5,996 tube wells in the 25-km(2) area of Araihazar, Bangladesh; 11,746 were recruited into the study. This analysis is based on 10,628 subjects (90.5%) with nonmissing dietary data. Skin lesions were identified according to a structured clinical protocol during screening and confirmed with further clinical review.. Riboflavin, pyridoxine, folic acid, and vitamins A, C, and E significantly modified risk of arsenic-related skin lesions. The deleterious effect of ingested arsenic, at a given exposure level, was significantly reduced (ranging from 46% reduction for pyridoxine to 68% for vitamin C) for persons in the highest quintiles of vitamin intake.. Intakes of B-vitamins and antioxidants, at doses greater than the current recommended daily amounts for the country, may reduce the risk of arsenic-related skin lesions in Bangladesh.

Topics: Adult; Antioxidants; Arsenic; Ascorbic Acid; Bangladesh; Cross-Sectional Studies; Environmental Exposure; Female; Humans; Male; Middle Aged; Precancerous Conditions; Risk Factors; Skin Diseases; Vitamin A; Vitamin B Complex; Vitamin E

Topics: Adult; Ascorbic Acid; Contusions; Female; Humans; Leg; Scurvy; Skin Diseases

We report a 23-year-old female patient with a 4-month history of transitory pigmented purpuric dermatoses (PPD). She was otherwise healthy and reported no history of previous medication intake and none of her family members had any disorders. Clinical examination revealed well-demarcated, brownish hyperpigmented, reticulated pigmentation with pinhead-sized purpura. The histopathological specimen was characterized by a mild epidermal hyperkeratosis, elongated rete ridges, papillomatosis and mild mononuclear cell infiltration in the superficial dermis with focal extravasations of red blood cells without siderophage. Despite prominent extravasations of red blood cells and edema both in the papillary dermis and the subpapillary layer, no definite capillaritis was seen. Based on these clinicohistopathological findings, the diagnosis of transitory PPD was considered to be most compatible. Clinicians should recognize the unique but rarely acknowledged disease as a subtype of pigmented purpuric dermatoses.

Topics: Adult; Ascorbic Acid; Asian People; Female; Humans; Pantothenic Acid; Purpura; Skin; Skin Diseases; Treatment Outcome

Chronically elevated blood glucose levels result in reduced leukocyte function and cell malnutrition, which contribute to a high rate of wound infection and associated healing problems in diabetic patients. In the present study, the role of biotinylated GHK peptide (BioGHK) incorporated collagen biomaterial was tested for wound healing in diabetic rats. The rate of wound contraction and the levels of collagen, uronic acid, protein and DNA in the granulation tissue were determined. Further, the concentration of nitric oxide and other skin antioxidants was also monitored during the study. In diabetic rats treated with BioGHK incorporated collagen (Peptide Incorporated Collagen--PIC), the healing process was hastened with an increased rate of wound contraction. Glutathione (GSH) and ascorbic acid levels in the skin of streptozotocin-induced diabetic rats were higher in the PIC group as compared to control (Untreated) and collagen (Collagen Film--CF) treated groups. Superoxide dismutase (SOD) and catalase (CAT) activity was altered in all the groups. In vitro fibroblast cell culture studies suggest that PIC promotes fibroblast growth. Histological evaluation by haematoxylin-eosin and Masson's trichrome method revealed epithelialization, increased synthesis of collagen and activation of fibroblasts and mast cells in the PIC group. This study provides a rationale for the topical application of BioGHK incorporated collagen as a feasible and productive approach to support diabetic wound healing.

Topics: Achilles Tendon; Animals; Ascorbic Acid; Biotinylation; Catalase; Cattle; Cells, Cultured; Collagen; Diabetes Mellitus, Experimental; Fibroblasts; Glutathione; Mast Cells; Matrix Metalloproteinases; Oligopeptides; Rats; Rats, Wistar; Skin Diseases; Superoxide Dismutase; Wound Healing

Topics: Administration, Oral; Animals; Ascorbic Acid; Cysteine; Disease Models, Animal; Drug Therapy, Combination; Guinea Pigs; Male; Riboflavin; Skin; Skin Diseases; Sodium Dodecyl Sulfate; Vitamin B 6

Topically applied antioxidants constitute an important group of protective agents against skin damage induced by ultraviolet radiation. The current study was performed to investigate whether a recently developed ex vivo pig skin model was suitable for short-term studies of the mechanism(s) of UVB-radiation-induced skin damage; the protective effect of topical application of alpha-tocopherol, l-ascorbic acid, alpha-lipoic acid, glutathione ethylester and N-acetylcysteine was tested. Increasing doses of the antioxidants were applied topically on ex vivo pig skin explants and allowed to penetrate for 60 min. Epidermal antioxidant bioavailability was measured before and 60 min after exposure to an ultraviolet B (UVB) radiation of 7.5 kJ/m2. Cell viability (trypan blue dye exclusion) and apoptosis were measured 48 h later in isolated keratinocytes. UVB-radiation-induced epidermal lipid peroxidation was determined immediately after exposure of the skin to a UVB dose of 28 kJ/m2. All antioxidants tested became bioavailable in pig skin epidermis, and none of them were depleted after UVB-radiation exposure. Increasing doses of the antioxidants tested decreased UVB-radiation-induced cell death and apoptosis. The highest doses of antioxidants prevented UVB-radiation-induced lipid peroxidation; alpha-lipoic acid only tended to decrease lipid peroxidation. In conclusion, a single topical dose of the above antioxidants on ex vivo pig skin can reduce UVB-radiation-induced oxidative stress and lipid peroxidation and thereby reduce apoptotic stimuli and cell death. Furthermore, the ex vivo pig skin model was a useful tool for testing compounds for their antioxidant activity.

Topics: Acetylcysteine; Administration, Topical; alpha-Tocopherol; Animals; Antioxidants; Apoptosis; Ascorbic Acid; Biological Availability; Culture Techniques; Epidermis; Glutathione; Lipid Peroxidation; Skin; Skin Diseases; Swine; Thioctic Acid; Treatment Outcome; Ultraviolet Rays

Topics: Abdomen; Ascorbic Acid; Humans; Male; Middle Aged; Purpura; Scurvy; Skin Diseases

About 10 million industrial workers of both sexes are exposed to metal-working fluids (MWFs) via inhalation, skin or both. Our preliminary results, following dermal application of 200 microl of 50% unused (neat) semisynthetic MWF (pH 7 or pH 9.7) to the unshaved backs of 6-wk-old B6C3F1 mice, twice a week for 6 wk, produced significant increase in weights of the liver of both sexes. The purpose of the present study was to determine if this weight change was related to oxidative stress subsequent to MWF exposure and also to determine whether ethanol intake influences this effect. Therefore, 6-mo-old mice of both sexes were exposed to MWFs following the protocol just described, except that the topical application was with 5% MWFs (pH 7 and 9.7, 5 d/wk) with or without adding 5% ethanol to their drinking water (7 d/wk) for 13 wk. The skin histamine levels and mast-cell numbers were significantly increased in the female group treated with 55% MWF (pH 7). The ascorbic acid levels in the liver (both sexes) (all groups except 5%, MWF pH 9.7 males) and testes were reduced significantly. Malondialdehyde levels in the male liver were significantly increased with topical MWF exposure. Glutathione levels were reduced significantly in both male and female liver after 5% MWF (pH 7). Alcohol dehydrogenase activity of the male liver increased significantly after MWF (pH 7). These results suggest that MWFs are absorbed through the skin and produce toxicity in the liver of both sexes and in the male gonads. This may represent an important health risk to MWF-exposed industrial workers, and ethanol may exacerbate this risk.

Topics: Alcohol Dehydrogenase; Animals; Ascorbic Acid; Female; Glutathione; Histamine; Indicators and Reagents; Industrial Oils; Male; Malondialdehyde; Mast Cells; Metallurgy; Mice; Skin Diseases

Topics: Adult; Ascorbic Acid; Blood Cell Count; Hair Diseases; Humans; Joint Diseases; Male; Scurvy; Skin; Skin Diseases

Topics: Adult; Ascorbic Acid; Diagnosis, Differential; Glomerulonephritis, Membranoproliferative; Humans; Male; Oxalates; Radiography; Renal Dialysis; Skin Diseases; Synovial Fluid

Topics: Ascorbic Acid; Chemotactic Factors; Chemotaxis, Leukocyte; Dipeptides; Humans; Neutrophils; Prednisolone; Skin Diseases; Tetracycline

The role of vitamins A, E, C, D, folates and selenium in the chemical prevention of tumours and/or precancerous conditions is examined in the light of epidemiological studies and experimental observations. Particular mention is made of significant clinical studies that provide valuable indications about the use of vitamin A and its derivates in particular for the treatment of precancerous and cancerous conditions. Vitamin A and its derivates apparently play a fundamental role not only in the treatment of proliferating malignancies of the skin (carcinomas, severe aclinic keratosis) or involving the skin (fungoid mycosis, skin metastases of solid tumours) but also in the prevention of recurring bladder tumours and the treatment of several bronchial dysplasias.

Topics: Ascorbic Acid; Folic Acid; Humans; Neoplasms; Retinoids; Selenium; Skin Diseases; Vitamin A; Vitamin D; Vitamin E; Vitamins

Topics: Adult; Ascorbic Acid; Down Syndrome; Female; Humans; Middle Aged; Nicotinic Acids; Skin Diseases; Thiamine; Vitamin A; Vitamin E; Vitamins; Zinc

Topics: Administration, Oral; Adult; Ascorbic Acid; Calcium; Drug Combinations; Female; Humans; Male; Methods; Phosphorous Acids; Plant Extracts; Regional Blood Flow; Skin; Skin Diseases; Skin Temperature; Vascular Diseases; Vitamin D

Topics: Adolescent; Adult; Ascorbic Acid; Ascorbic Acid Deficiency; Capillary Fragility; Cold Climate; Female; Humans; Male; Middle Aged; Psoriasis; Skin Diseases

Topics: Adult; Ascorbic Acid; Black People; Dental Caries Susceptibility; Fatty Acids; Gingivitis; Health Surveys; Humans; Keratosis; Male; Middle Aged; Nicotinic Acids; Pellagra; Riboflavin Deficiency; Rural Population; Skin Diseases; South Africa; Urban Population; Vitamin A Deficiency

Topics: Adult; Aged; Ascorbic Acid; Caproates; Citrates; Female; Flavonoids; Frostbite; Humans; Male; Middle Aged; Nucleosides; Phlebitis; Rutin; Skin Diseases; Telangiectasis; Varicose Ulcer; Varicose Veins; Vascular Diseases

Topics: Ascorbic Acid; Capillary Resistance; Flavonoids; Humans; Rutin; Skin Diseases; Vitamin K

Topics: Adolescent; Adult; Aged; Ascorbic Acid; Citrates; Female; Flavonoids; Hemorrhoids; Humans; Leg Ulcer; Male; Middle Aged; Nucleosides; Skin Diseases; Varicose Veins; Vascular Diseases

Topics: Ascorbic Acid; Humans; Keratosis; Skin Diseases; Vitamin A; Vitamin B Complex; Vitamins

Topics: Animal Feed; Animals; Animals, Newborn; Ascorbic Acid; Ascorbic Acid Deficiency; Diet; Skin Diseases; Swine; Swine Diseases; Vitamin A; Vitamin A Deficiency

Topics: Ascorbic Acid; Carbohydrate Metabolism; Hemochromatosis; Humans; Iron; Scurvy; Skin Diseases; Vitamins

Topics: Ascorbic Acid; Humans; Pigmentation; Pigmentation Disorders; Skin Diseases

Topics: Anti-Bacterial Agents; Antibiotics, Antitubercular; Ascorbic Acid; Skin Diseases; Tuberculosis; Tuberculosis, Pulmonary

Topics: Animals; Ascorbic Acid; Contusions; Humans; Poultry; Skin; Skin Diseases; Vitamins; Wound Healing

Topics: Anti-Allergic Agents; Antineoplastic Agents; Ascorbic Acid; Flavonoids; Histamine H1 Antagonists; Humans; Skin Diseases; Urticaria; Vitamins

Topics: Ascorbic Acid; Calcium; Calcium, Dietary; Dermatology; Skin Diseases; Vitamins

Topics: Ascorbic Acid; Flavonoids; Humans; Skin Diseases; Vitamins

Topics: Ascorbic Acid; Humans; Hypersensitivity; Leukocytes; Skin Diseases

Topics: Animals; Ascorbic Acid; Guinea Pigs; Humans; Skin; Skin Diseases; Vitamins; Wound Healing

Topics: Ascorbic Acid; Disease; Humans; Mucous Membrane; Skin Diseases; Vitamin A; Vitamins