ascorbic-acid and Ovarian-Diseases

The aim of the study was to evaluate the protective efficacy of ascorbic acid, α-tocopherol, and selenium by measuring the glutathione (GSH) levels and proliferating cell nuclear antigen (PCNA) and growth differentiation factor-9 (GDF-9) expression in the ovarian tissues of rats treated with cyclophosphamide (CP) therapy.. Female Wistar rats were divided into 5 groups of 6 rats each: (I) control, (II) only CP, (III) CP + ascorbic acid, (IV) CP + α-tocopherol, and (V) CP + selenium. Immunohistochemical stainings and GSH protocol were then applied.. Following CP administration, the rats exhibited significantly lower GDF-9 expression in oocytes and PCNA expression in granulosa cells of follicles in all stages of development (P < 0.05). In CP + antioxidant groups (Groups III, IV, V), GDF-9 immunoreaction in oocytes and PCNA immunoreaction in granulosa cells of the developing follicles were found to show an increase towards the levels observed in the control group (P < 0.05).. CP was found to cause remarkable degenerative effects in normal ovarian tissue, and we believe that this damage can be reduced and ovarian tissue can be spared from the toxic effects of CP by using antioxidants such as ascorbic acid, α-tocopherol, and selenium.

Topics: alpha-Tocopherol; Animals; Antineoplastic Agents, Alkylating; Antioxidants; Ascorbic Acid; Cyclophosphamide; Female; Glutathione; Granulosa Cells; Growth Differentiation Factor 9; Oocytes; Ovarian Diseases; Ovary; Proliferating Cell Nuclear Antigen; Rats; Rats, Wistar; Selenium

To study the levels of four markers of oxidative stress in follicular fluid (FF) and plasma of patients with infertility related to endometriosis and controls.. Experimental study.. University-affiliated hospital and infertility center.. Ninety-one infertile women were included in the study (23 infertile women with endometriosis and 68 controls including infertile women due to tubal factor, male factor, or healthy egg donors).. Blood was obtained at the time of egg retrieval, and FF from the mature follicles of each ovary was centrifuged and frozen until analysis.. Vitamin C and E, malondialdehyde, and superoxide dismutase concentrations in plasma and follicular fluid.. Women with endometriosis showed a lower vitamin C concentration in FF (12.7 ± 5.9 vs. 9.7 ± 6.9 μg/mL) and lower superoxide dismutase concentration in plasma (0.9 ± 1.4 vs. 0.5 ± 0.7 U/mL) compared with controls. Vitamin E plasma levels were significantly higher in women with endometriosis (8.1 ± 3.8 vs. 5.2 ± 3.2 μg/mL). A nonsignificant trend toward a lower plasma concentration of malondialdehyde was found in women with endometriosis.. These findings suggest a lower antioxidant capacity in infertile women with endometriosis. Although a certain level of reactive oxygen species is required under physiological conditions, an altered balance between pro-oxidant and antioxidant activities may have an impact on folliculogenesis and adequate embryo development.

Topics: Adult; Ascorbic Acid; Biomarkers; Case-Control Studies; Endometriosis; Female; Follicular Fluid; Humans; Infertility, Female; Male; Ovarian Diseases; Oxidative Stress; Superoxide Dismutase; Superoxide Dismutase-1

We have investigated the effect of subchronic administration of methidathion (MD) on ovary evaluated ameliorating effects of vitamins E and C against MD toxicity. Experimental groups were as follows: control group; a group treated with 5 mg/kg body weight MD (MD group); and a group treated with 5 mg/kg body weight MD plus vitamin E and vitamin C (MD + Vit group). MD and MD + Vit groups were given MD by gavage five days a week for four weeks at a dose level of 5 mg/kg/day by using corn oil as the vehicle. Serum malondialdehyde (MDA: an indicator of lipid peroxidation) concentration, serum activity of cholinesterase (ChE), and ovary histopathology were studied. The level of MDA increased significantly in the MD group compared with the control (P < 0.005). Serum MDA decreased significantly in the MD + Vit group compared with the MD group (P < 0.05). The activities of ChE decreased significantly both in the MD and MD + Vit groups compared with the controls ( P < 0.05). However, the decrease in the MD + Vit groups was less than in the MD group; the ChE activity in the MD + Vit group was significantly higher compared with MD group (P < 0.05). Number of ovarian follicles were significantly lower in the MD group compared to the controls (P < 0.05). Number of atretic follicles were significantly higher in the MD group than in the controls (P < 0.05). Follicle counts in MD + Vit group showed that all types of ovarian follicles were significantly higher, and a significant decrease in the number of atretic follicles compared with the MD group (P < 0.05). In conclusion, subchronic MD administration caused an ovarian damage, in addition, LPO may be one of the molecular mechanisms involved in MD-induced toxicity. Treatment with vitamins E and C after the administration of MD reduced LPO and ovarian damage.

Topics: Administration, Oral; alpha-Tocopherol; Animals; Ascorbic Acid; Cholinesterases; Corpus Luteum; Drug Administration Schedule; Drug Therapy, Combination; Estrous Cycle; Fatigue; Female; Injections, Intraperitoneal; Insecticides; Intubation, Gastrointestinal; Malondialdehyde; Organothiophosphorus Compounds; Ovarian Diseases; Ovarian Follicle; Rats; Rats, Wistar; Tocopherols; Vitamins; Weight Gain; Weight Loss

A course of ascovertin therapy reduced manifestations of perifocal inflammation and atresia of growing follicles, corrected vascular disorders and changes in the tissue volume ratios outside the necrotic zone after monopolar coagulation of the ovaries in rats. The drug prevented the increase in blood viscosity, erythrocyte aggregation, and LPO activation in the early postoperation period.

Topics: Animals; Antioxidants; Ascorbic Acid; Blood Viscosity; Drug Combinations; Erythrocyte Aggregation; Erythrocytes; Fallopian Tubes; Female; Flavonols; Gynecologic Surgical Procedures; Hematocrit; Ovarian Diseases; Ovary; Postoperative Period; Quercetin; Rats

The sera of 26 patients with premature ovarian failure were examined in order to detect immunoglobulin-G (IgGs) that can block FSH-induced in vitro granulosa cell DNA synthesis via, a Feulgen cytochemical bioassay system. The IgGs of four patients with polycystic ovary-like disease, five postmenopausal women, and four eumenorrheic women served as controls. Ovarian growth blocking IgGs were found in 21 of the 26 premature ovarian failure (POF) cases. The few cases characterized by the absence of follicles (streak ovaries) and the controls were negative. The ovarian blocking IgGs were far more prevalent in the POF cases than anti-cytoplasmic ovarian antibodies detected by an indirect immunofluorescence assay (only one of the 26 POF patients was positive). Our data hence confirm earlier expressed views that immune mechanisms are involved in a high proportion of patients with POF.

Topics: Adult; Ascorbic Acid; Autoimmune Diseases; DNA Replication; Female; Follicle Stimulating Hormone; Granulosa Cells; Humans; Immunoglobulin G; Immunoglobulins; In Vitro Techniques; Kinetics; Luteinizing Hormone; Menopause; Menopause, Premature; Menstruation; Ovarian Diseases; Radioimmunoassay; Reference Values

Topics: Animals; Ascorbic Acid; Atrophy; Biological Assay; Female; Luteinizing Hormone; Mestranol; Norethynodrel; Organ Size; Ovarian Diseases; Ovary; Pituitary Gland; Rats

Topics: Adrenal Glands; Animals; Ascorbic Acid; Biological Assay; Chemosterilants; Cysts; Estrus; Female; Follicle Stimulating Hormone; Gonadotropins, Pituitary; Infertility, Female; Luteinizing Hormone; Organ Size; Ovarian Diseases; Ovary; Pituitary Gland; Pregnancy; Rats; Testosterone