ascorbic-acid and Mycoses

A series of novel coumarin pyrazole hybrids of biological interest were synthesized from the hydrazones, carbazones and thiocarbazones via Vilsmeier Haack formylation reaction. These intermediates and formyl pyrazoles were evaluated for antimicrobial and antioxidant activities. Among the series, compounds 6g and 6h showed excellent antimicrobial activity against different bacterial and fungal strains and compounds 7g, 7h were found to be potent antioxidant agents in both DPPH and hydroxyl radical scavenging assays. Further, detailed quantitative structure-activity relationship (QSAR) analysis indicated the molecular parameters that contribute to increased potency of inhibition. The above findings would further encourage our understanding in employing coumarin pyrazole hybrids as potential antibiotic agents for treating infections caused by pathogenic microbes and fungi. Further, it also paves the way for exploration of these compounds as potential therapeutic agents to treat conditions arising because of excessive oxidative damage.

Topics: Anti-Infective Agents; Antioxidants; Bacteria; Bacterial Infections; Coumarins; Fungi; Humans; Microbial Sensitivity Tests; Mycoses; Pyrazoles; Quantitative Structure-Activity Relationship

A series of 1,2,4-(triazolo[3,4-b][1,3,4]thiadiazol-6-yl)selenopheno[2,3-d]pyrimidines (10a-j) were synthesized with various substituted anilines and benzoic acids. Structures of newly synthesized compounds were established by IR, (1)H &(13)C NMR and LC-MS spectral data. The antioxidant activity of the synthesized compounds was evaluated by DPPH, NO and H2O2 radical scavenging methods. The newly synthesized compounds were evaluated for their antimicrobial activity against Gram +ve and Gram -ve bacteria and antifungal activity by well diffusion method. Compounds 10d, 10h and 10i showed promising antioxidant, antibacterial as well as antifungal activity and these were found to be the most potent activity molecules when compared with that of standard drugs. Molecules docking studies have been performed on Staphylococcus aureus (SA) of Gram +ve bacteria.

Topics: Anti-Infective Agents; Antioxidants; Bacteria; Bacterial Infections; Benzene Derivatives; Fungi; Humans; Microbial Sensitivity Tests; Molecular Docking Simulation; Mycoses; Organoselenium Compounds; Pyrimidines; Thiadiazoles

The chemical composition of the essential oil obtained from Teucrium marum subsp. marum (Lamiaceae) was analysed by GC/MS and 30 components were identified. Isocaryophyllene (20.24%), beta-bisabolene (14.73%), beta-sesquiphellandrene (11.27%), alpha-santalene (10.97%), dolichodial (9.38%) and, alpha-caryophyllene (7.18%) were the main components. The antimicrobial activity of the essential oil was assayed against four phytopathogenic fungi and Rhyzoctonia solani resulted to be the most sensitive microorganism with a MIC value of 250 ppm. The antioxidant activity of the essential oil was evaluated using the DPPH test, 5-lipoxygenase test and luminol/xanthine/xanthine oxidase chemiluminescence assay.

Topics: Antioxidants; Ascorbic Acid; Biphenyl Compounds; Butylated Hydroxytoluene; Chromans; Drug Evaluation, Preclinical; Fatty Acids; Free Radical Scavengers; Fungicides, Industrial; Inhibitory Concentration 50; Microbial Sensitivity Tests; Mitosporic Fungi; Monoterpenes; Mycoses; Picrates; Plant Components, Aerial; Plant Diseases; Plant Oils; Sesquiterpenes; Superoxides; Teucrium

Amphotericin B is used for the treatment of systemic mycoses and visceral leishmaniasis. The objective of our study was to evaluate the impact of catalase, ascorbic acid and ketoconazole on the amphotericin B toxicity towards Leishmania promastigotes membrane by two flow cytometric tests, the membrane potential assay using a cationic dye, [DiOC5(3)], and the membrane permeability test using propidium iodide. The collapse of membrane potential appeared at amphotericin B concentrations weaker than those assessed by the membrane permeability test. The binding of amphotericin B to membrane sterol was not modified by catalase or ascorbic acid whereas amphotericin B-induced growth inhibition could be modulated by these products. The permeabilizing effect of amphotericin B on parasite membrane was strongly reduced in the presence of ketoconazole. These results confirmed the pore hypothesis of amphotericin B action and suggested that flow cytometric methods constituted a valuable alternative to conventional methods for assessing the effect of drugs on cellular membrane and evaluating parasite susceptibility to polyene antibiotics.

Topics: Amphotericin B; Animals; Antifungal Agents; Antioxidants; Antiprotozoal Agents; Ascorbic Acid; Catalase; Cell Membrane; Cell Membrane Permeability; Flow Cytometry; Ketoconazole; Leishmania infantum; Leishmaniasis, Visceral; Membrane Potentials; Mycoses; Parasitic Sensitivity Tests

Topics: Adolescent; Ascorbic Acid; Dietary Proteins; Eating; Energy Intake; Female; Female Urogenital Diseases; Humans; Incidence; Iron; Mycoses; Pregnancy; Pregnancy Complications, Infectious; Pregnancy in Adolescence; Sexually Transmitted Diseases; Urinary Tract Infections; Vitamin A; Zinc

Topics: Adjuvants, Immunologic; Antiviral Agents; Ascorbic Acid; Bacterial Infections; Glucans; Humans; Hypersensitivity, Delayed; Immune System Diseases; Inosine; Inosine Pranobex; Levamisole; Lymphocyte Activation; Mycoses; Phagocytosis; Rosette Formation; T-Lymphocytes; Thymus Hormones; Tilorone; Transfer Factor; Virus Diseases

Topics: Ascorbic Acid; Balanitis; Candidiasis, Oral; Carbonates; Female; Gastritis; Gingivitis; Herpes Simplex; Humans; Leukoplakia; Male; Mycoses; Osteomyelitis; Pressure Ulcer; Stomatitis; Tonsillitis; Vulvitis