ascorbic-acid and Leukocytosis

Topics: Anemia; Ascorbic Acid; Blood Coagulation Disorders; Chronic Disease; Folic Acid; Hematologic Diseases; Hemolysis; Humans; Leukocytosis; Mononuclear Phagocyte System; Serum Albumin; Stress, Physiological; Syndrome; Thrombocytosis; Zinc

An increased systemic concentration of stress hormones (of the hypothalamic-pituitary adrenal axis) and some cytokines may contribute to the depression of immune cell function typically observed after prolonged exercise. The aim of the present study was to determine the effect of 2 weeks of supplementation with vitamin C (VC) on cortisol, adrenocorticotrophic hormone, interleukin-6, oxidative stress and neutrophil responses to a single bout of endurance exercise. Nine healthy endurance-trained males exercised for 2.5 h at 60% VO2max after 2 weeks of placebo (PLA) or VC (1,000 mg day(-1)) supplementation. All participants completed both trials utilising a randomised crossover design with a minimum 14 day washout period between trials. There was a significant trial x time interaction effect for plasma cortisol concentration (P = 0.039) which tended to be lower in the VC trial but post hoc analysis found no specific between trial differences. There was a significantly lower post-exercise neutrophilia (P < 0.014) in the VC trial, compared with the PLA trial. There was no trial x time interaction for measures of neutrophil function (bacteria-stimulated elastase release, fMLP or PMA-stimulated oxidative burst). However, there was a trend for higher fMLP-stimulated neutrophil oxidative burst in the VC compared with PLA trial (trial x time interaction, P = 0.075). These results suggest that supplementation with VC for a period of up to 2 weeks provides little to no protection against the depression of neutrophil function which typically occurs after endurance exercise.

Topics: Adrenocorticotropic Hormone; Antioxidants; Ascorbic Acid; Bicycling; Blood Glucose; Dietary Supplements; Humans; Hydrocortisone; Interleukin-6; Leukocyte Count; Leukocytosis; Male; Neutrophils; Physical Endurance; Respiratory Burst

To study the immune-modulating and anti-oxidant effects of beta-glucan, papaya, lactoferrin, and vitamins C and E on sperm characteristics of patients with asthenoteratozoospermia associated with leucocytosis.. Fifty-one patients referred to our Sterility Center for semen analysis were selected. Sperm parameters were assessed before and after patient's treatment with beta-glucan, lactoferrin, papaya, and vitamins C and E. DNA damage was assessed by the acridine orange test and sperm structural characteristics were evaluated by transmission electron microscopy.. After 90 days of treatment, an increase in the percentage of morphologically normal sperm (17.0 +/- 5.2 vs. 29.8 +/- 6.5) and total progressive motility (19.0 +/- 7.8 vs. 34.8 +/- 6.8) were detected. Structural sperm characteristics as well as chromatin integrity were also improved after treatment. In terms of leukocyte concentration in seminal fluid, a significant reduction was recorded (2.2 +/- 0.9 vs. 0.9 +/- 0.2).. The treatment of an inflammatory process by the synergic action of immune modulators and anti-oxidants could protect sperm during maturation and migration, leading to improved sperm function.

Topics: Adult; Antioxidants; Ascorbic Acid; Asthenozoospermia; beta-Glucans; Carica; Humans; Lactoferrin; Leukocytosis; Male; Middle Aged; Phytotherapy; Plant Preparations; Spermatozoa; Treatment Outcome; Vitamin E

Leukocytes are often present in human seminal plasma and more frequently in infertile men. Leukocytospermia is associated with sperm morphological and functional alterations. Immune cell activation leads to an increase of free radical production, without any antioxidant defence activation. Leukocyte presence during sperm maturation and migration through male genital tract and consequently exposure to reactive oxygen species led to sperm alteration: axonemal, acrosomal and nuclear structure damage, associated with necrosis. In order to evaluate the immune-modulating and antioxidative activity of beta-glucan, fermented papaya and lactoferrin associated with vitamins C and E, we analysed sperm characteristics of selected infertile male with astheno-teratospermia and abacterial leukocytosis.. We selected 20 patients referred to our Sterility Centre for semen analysis with leukocyte concentration higher than 1x106 cell/mL. Seminal quality evaluation was performed according to WHO guidelines (1999) using Papanicolau and eosin staining, before and after three months of treatment with beta-glucan, papaya, lactoferrin, vitamin C and E.. After therapy, seminal analysis showed a significant reduction of leukocyte concentration and an increase of sperm motility and normal sperm morphology.. Our results suggest that a combined immunomodulating and antioxidant treatment protect sperm cells during maturation and migration through the male genital tract, resulting in a functional rescue demonstrated by the improvement of semen quality.

Topics: Adjuvants, Immunologic; Adult; Antioxidants; Ascorbic Acid; beta-Glucans; Carica; Case-Control Studies; Drug Therapy, Combination; Fruit; Humans; Infertility, Male; Lactoferrin; Leukocytosis; Male; Middle Aged; Phytotherapy; Spermatozoa; Treatment Outcome; Vitamin E

The changes in buffy layer (BL) vitamin C concentration following surgical operations were studied in relation to changes in the leucocyte and platelet counts. It was found that the postoperative fall and subsequent changes in BL vitamin C concentration corresponded to changes in the platelet/leucocyte ratio (PLR) in whole blood and consequently in the buffy layer preparations from which vitamin C concentrations were measured. The results of the study showed that the fall in BL vitamin C measured following operations is an artifact of the method used rather than a demand for vitamin C. It highlights the importance of measuring the PLR, and suggests the need for a differential leucocyte count, when undertaking BL vitamin C analysis.

Topics: Adult; Aged; Ascorbic Acid; Blood Platelets; Blood Transfusion; Female; Humans; Leukocyte Count; Leukocytes; Leukocytosis; Male; Middle Aged; Platelet Count; Postoperative Period

Topics: Adult; Aged; Ascorbic Acid; Cell Adhesion; Female; Granulocytes; Humans; Leukocyte Count; Leukocytosis; Lithium; Male; Middle Aged; Neutrophils

The postoperative ascorbic acid requirements of 63 surgical patients were assessed by measurements of buffy layer leukocyte ascorbic acid and the ascorbic acid content of leukocytes. There was a significant reduction in ascorbic acid levels following operation. The postoperative changes were unrelated to the extent of surgical trauma or the volume of blood transfused during operation, but there was a significant correlation between postoperative ascorbic acid measurements and white blood counts. It appears that postoperative leukocytosis and release by the bone marrow of leukocytes with a low ascorbic acid content may partly account for the postoperative changes in buffy layer and leukocyte ascorbic acid measurements. However, surgical operations were followed by an authentic increase in ascorbic acid requirements, and there was a 42 per cent reduction in circulating leukocyte ascorbic acid levels on the third postoperative day. The findings of this study create an argument for the use of ascorbic acid supplements in surgical patients, although it is unlikely that postoperative changes in leukocyte ascorbic acid have pathologic significance in wound repair.

Topics: Aged; Ascorbic Acid; Blood Transfusion; Bone Marrow Cells; Diet; Humans; Leukocyte Count; Leukocytes; Leukocytosis; Middle Aged; Postoperative Care

White-blood counts, polymorph counts, leucocyte ascorbic-acid and plasma ascorbic-acid levels were measured in 91 samples of blood obtained from patients admitted to a geriatric assessment unit. There was a correlation between both white-blood counts and polymorph counts and leucocyte ascorbic-acid levels. There was no correlation between white-blood counts and polymorph counts and plasma ascorbic-acid concentrations. It is suggested that this phenomenon represents migration of ascorbate-rich polymorphs to areas of infection and infarction.

Topics: Aged; Aging; Ascorbic Acid; Female; Humans; Leukocyte Count; Leukocytes; Leukocytosis; Male

Topics: Anemia; Anemia, Hypochromic; Ascorbic Acid; Blood Transfusion; Deficiency Diseases; Humans; Infant; Iron; Leukocytosis; Rickets; Splenomegaly; Vitamin D; Vitamins

Topics: Adrenal Glands; Adrenocorticotropic Hormone; Animals; Ascorbic Acid; Eosinophils; Epinephrine; Hypothalamus; Leukocytosis; Pituitary Gland; Pituitary Gland, Anterior; Rats