ascorbic-acid and Leprosy

Topics: Adult; Animals; Ascorbic Acid; Asia, Southeastern; Child; Child, Preschool; Cricetinae; Humans; Immune System Diseases; Immunity, Cellular; Leprosy; Mice; Nutrition Disorders; Protein-Energy Malnutrition; Rats; Risk

Healing of chronic plantar ulcers in leprosy (CPUL) typically takes a long time due to impaired neurological function, thereby reducing the levels of growth factors and cytokines. Cytokines can be found in metabolite products from amniotic membrane stem cells. Chronic ulcers are frequently characterized by high levels of reactive oxygen species. Vitamin E (α-tocopherol) is widely used in skin lesions, owing to its antioxidant and anti-inflammatory properties. Vitamin C also has antioxidant, anti-inflammatory, and collagen synthesis properties which are useful in wound healing. Herein, we compared the effects of topical human amniotic membrane-mesenchymal stem cell-conditioned medium (hAMMSC-CM) alone and with vitamins C and E on healing of CPUL.. In this randomized controlled trial, topical agents were applied every 3 days for up to 8 weeks. Ulcer size, side-effects, and possible complications were monitored weekly.. Healing percentage increased each week in all groups. Mean difference in ulcer size was highest in the hAMMSC-CM + vitamin E group, implying better progress of wound healing. There were no side-effects or complications.. hAMMSC-CM + vitamin E is best for healing of CPUL.

Topics: Adult; Amnion; Ascorbic Acid; Culture Media, Conditioned; Female; Foot Ulcer; Humans; Leprosy; Male; Mesenchymal Stem Cells; Middle Aged; Vitamin E; Wound Healing; Young Adult

Leprosy is a chronic infectious disease with many risk factors including inadequate nutrient intake and nutritional deficiencies, which affect the immune system, and influence leprosy progression.. To elucidate the relation between the serum level of zinc, vitamin C, and selenium and the clinical spectrum of leprosy.. A case control study included 100 leprotic patients (50 multibacillary and 50 paucibacillary) and 100 age and sex matched controls. Vitamin C was measured by ELISA, zinc was measured by using centronic colorimetric spectrophotometry, and selenium was measured by Inductivity Coupled Plasma Optical Emission Spectroscopy technique.. Zinc and Vitamin C levels were significantly lower in paucibacillary (mean ± SD = 89.86 ± 20.712 and 2.52 ± 1.27 respectively) and multibacillary (mean ± SD = 81.41 ± 18.61 and 1.98 ± 0.59 respectively) than in controls (mean ± SD = 107.34 ± 3.98 and 4.95 ± 2.45 respectively) (p value < 0.001) with no significant difference between paucibacillary and multibacillary patients (p value = 0.142 and = 0.066 respectively). Selenium level showed no significant difference between the three groups (p value > 0.05) (mean ± SD = 51.27 ± 42.61 in paucibacillary, 47.54 ± 30.21 in multibacillary, and 44.07 ± 46.58 in controls).. Lower serum levels of zinc and vitamin C in leprosy patients may be a result of disease pathogenesis or related to the antioxidants based treatment. It might also present prior to the disease onset due to malnutrition that may have accelerated the development of leprosy.

Topics: Ascorbic Acid; Case-Control Studies; Humans; Leprosy; Malnutrition; Selenium; Zinc

The basal level of blood ascorbic acid was studied in 70 cases of polar leprosy. The level was found to be significantly reduced in both polar types, more so in tuberculoid. In untreated cases of these two polar forms, the differences were highly significant. No significant differences were found between untreated and treated cases of either form of leprosy, indicating that dapsone (DDS) has no effect on the ascorbic acid level. In lepromatous leprosy, the level was not affected by erythema nodosum leprosum (ENL). The deficiency of ascorbic acid in leprosy might be due to the disease per se and/or associated with nutritional factors. In trophic ulcers after supplement therapy for 60 days, the low level of ascorbic acid became close to the control group with marked improvement in the healing of the ulcers clinically. In addition, lactic and pyruvic acids were also measured and found to be significantly raised. After ascorbic acid supplement, the levels fell to near the control values, indicating that ascorbic acid played a role in controlling the infection as well as possible thiamine deficiency and also checked the disturbance caused by DDS on lactate and pyruvate metabolism. The present study suggested that supplementing DDS therapy with ascorbic acid might provide a beneficial effect on the general condition of the leprosy patients and the early healing of trophic ulcers.

Topics: Ascorbic Acid; Dapsone; Erythema Nodosum; Humans; Leprosy; Skin Ulcer; Socioeconomic Factors

The stimulatory effects of ascorbate on neutrophil motility in vitro and in vivo and lymphocyte transformation to mitogens following ingestion or intravenous injection of ascorbate have been found to be related entirely to inhibition of the autooxidative effect of the myeloperoxidase/hydrogen peroxide/halide system (MPO/H2O2/halide system). Stimulation of neutrophil migration and lymphocyte transformation following a single intravenous injection of 1 g of ascorbate was associated with inhibition of the MPO/H2O2/halide system. The immunostimulatory activity and peroxidase inhibitory activity was related entirely to the serum ascorbate level. The relationship between inhibition of the peroxidase/h2O2/halide system and stimulation of neutrophil motility and lymphocyte mitogen-induced transformation was further established by using the horseradish peroxidase (HRP)/H2O2/halide system in vitro. Neutrophils and lymphocytes, exposed to this system, manifested markedly impaired chemotactic responsiveness and mitogen-induced transformation, respectively. However inclusion of ascorbate with the peroxidative system protected the neutrophils and lymphocytes from these inhibitory effects. Further studies in 3 patients with chronic granulomatous disease (CGD) and 10 patients with bronchial asthma suggested that ascorbate may be of value to improve the primary immunological abnormalities (neutrophil motility and antimicrobial activity) in CGD and the secondary abnormalities (neutrophil motility and lymphocyte transformation) found in some individuals with bronchial asthma.

Topics: Antibody Formation; Ascorbic Acid; Asthma; Child; Humans; Leprosy; Leukocytes; Lymphomatoid Granulomatosis; Neutrophils; Time Factors

A study was undertaken to probe into the influence of ascorbic acid on the absorption and metabolism of administered DDS in leprosy patients. Vit 'C' supplementation did not generally exhibit any effect on blood levels DDS except in cases of BB and LL where only 8 hrs DDS values showed a statistically significant increase.

Topics: Ascorbic Acid; Dapsone; Humans; Leprosy; Time Factors

Topics: Ascorbic Acid; Glucuronidase; Humans; Hyaluronic Acid; Leprosy; Lipid Metabolism; Mycobacterium leprae

Topics: Anti-Bacterial Agents; Antitubercular Agents; Ascorbic Acid; Bacteria; Cholera; Dapsone; Dysentery, Bacillary; Female; Heterocyclic Compounds; Humans; Leprosy; Meningitis; Meningitis, Haemophilus; Methods; Otorhinolaryngologic Diseases; Pregnancy; Puerperal Infection; Scarlet Fever; Sulfathiazoles; Sulfones; Tuberculosis, Meningeal; Tuberculosis, Pulmonary; Urinary Tract Infections; Vitamin K

Topics: Ascorbic Acid; Humans; Leprosy; Vitamins

Topics: Ascorbic Acid; Carbohydrate Metabolism; Humans; Leprosy