ascorbic-acid and Granulomatous-Disease--Chronic

ascorbic-acid has been researched along with Granulomatous-Disease--Chronic* in 12 studies

Reviews

2 review(s) available for ascorbic-acid and Granulomatous-Disease--Chronic

ArticleYear
Phagocytic cell dysfunction.
    The Journal of allergy and clinical immunology, 1986, Volume: 77, Issue:3

    Topics: Anti-Bacterial Agents; Ascorbic Acid; Blood Transfusion; Cell Adhesion; Chemotaxis; Female; Granulocytes; Granulomatous Disease, Chronic; Humans; Hypergammaglobulinemia; Immunoglobulin E; Luminescent Measurements; Neutrophils; Phagocyte Bactericidal Dysfunction; Phagocytes

1986
Modulation of tubulin tyrosinolation in human polymorphonuclear leukocytes (PMM).
    Kroc Foundation series, 1984, Volume: 16

    Topics: Anaerobiosis; Antioxidants; Ascorbic Acid; Carboxypeptidases; Chediak-Higashi Syndrome; Cyclohexanones; Diamide; Granulomatous Disease, Chronic; Humans; Methylene Blue; N-Formylmethionine Leucyl-Phenylalanine; Neutrophils; Oxidation-Reduction; Peptide Synthases; Protein Processing, Post-Translational; Tubulin; Tyrosine

1984

Trials

1 trial(s) available for ascorbic-acid and Granulomatous-Disease--Chronic

ArticleYear
Ascorbate (1g/day) does not help the phagocyte killing defect of X-linked chronic granulomatous disease.
    Clinical and experimental immunology, 1983, Volume: 51, Issue:1

    Topics: Adolescent; Ascorbic Acid; Candida; Cells, Cultured; Clinical Trials as Topic; Female; Granulomatous Disease, Chronic; Humans; Male; Neutrophils; Phagocytosis; Sex Chromosome Aberrations; X Chromosome

1983

Other Studies

9 other study(ies) available for ascorbic-acid and Granulomatous-Disease--Chronic

ArticleYear
Ascorbic acid neutralizes reactive oxidants released by hyperactive phagocytes from cigarette smokers.
    Lung, 1988, Volume: 166, Issue:3

    During exposure to the leukoattractant FMLP (N-formyl-L-methionyl-L-leucyl-L-phenylalanine) human polymorphonuclear leukocytes (PMNL) exhibit a bimodal pattern of luminol-enhanced chemiluminescence (LECL) with distinct early extracellular and later-occurring intracellular membrane-associated oxidative responses [4, 7, 14]. With the primary objective of measuring the effects of oral administration of the antioxidant ascorbate on the generation of reactive oxidants by circulating phagocytes from cigarette smokers and nonsmokers, we have developed a method for the measurement of FMLP-activated LECL in whole blood. With this method definite bimodal LECL responses, similar to those obtained with pure PMNL, were observed with FMLP-activated whole blood. No LECL responses were observed when whole blood from 3 children with chronic granulomatous disease was stimulated with FMLP, which shows that the FMLP-activated LECL responses are exclusively phagocyte-derived in blood from normal individuals. The whole blood method was used to compare the FMLP-activated LECL responses in blood from 30 asymptomatic smokers and 30 nonsmokers and to investigate the effects of co-incubation of whole blood from smokers and nonsmokers with ascorbate (2.5 X 10(-5) M-2.5 X 10(-4)M), as well as the effects of oral administration of the antioxidant on FMLP-activated LECL. Increased generation of both extracellular (58% mean increase, P less than 0.005) and intracellular (75% mean increase, P less than 0.005) phagocyte-derived oxidants was observed with FMLP-activated blood from smokers relative to nonsmokers. Co-incubation of blood with ascorbate in vitro caused a dose-dependent selective neutralization of extracellular oxidants. Similar effects were observed following the oral administration of a single dose of ascorbate (1 g). The whole blood method may be useful in identifying smokers at risk for smoking-related diseases.

    Topics: Adolescent; Adult; Ascorbic Acid; Child; Female; Granulomatous Disease, Chronic; Humans; Luminescent Measurements; Male; N-Formylmethionine Leucyl-Phenylalanine; Oxidation-Reduction; Phagocytes; Smoking

1988
Effect of iron on polymorphonuclear granulocyte phagocytic capacity: role of oxidation state and effect of ascorbic acid.
    British journal of haematology, 1988, Volume: 70, Issue:4

    It has been shown that iron (III) impairs the function of polymorphonuclear granulocytes (PMN). We have studied the effect of iron (II), on the membrane function of PMN, by assessing the uptake of radiolabelled Staphylococcus aureus by these cells. Iron (II), significantly impaired PMN phagocytic function. Addition of ascorbic acid reduced uptake further. Ferrous ascorbate, molar ratio 1:20, impaired phagocytic capacity of PMN significantly at iron concentrations as low as 1-10 microM. The toxic effect of iron (II) was not observed when desferrioxamine or transferrin was present in the incubation medium. The oxygen-free radical scavengers thiourea, mannitol and catalase prevented toxicity mediated by ferrous ammoniumsulphate but not by ferrous ascorbate (molar ratio of 1:20). Although high concentrations of ascorbic acid inhibited the generation of .OH and also the formation of the DMPO-.OH adduct by zymosan stimulated PMN, toxicity of iron increased. Iron (II) impaired the uptake of S. aureus by PMN of a patient with chronic granulomatous disease while iron (III) did not. Iron mediated impairment of PMN function is not only a result of the generation of toxic oxygen metabolites but also of direct interaction of iron (II) or an iron (II)-oxygen intermediate with molecules of the cell membrane.

    Topics: Adult; Ascorbic Acid; Ferrous Compounds; Granulomatous Disease, Chronic; Humans; Neutrophils; Oxidation-Reduction; Phagocytosis

1988
Association of neutrophil chemiluminescence with microbicidal activity.
    Clinical immunology and immunopathology, 1982, Volume: 22, Issue:2

    Topics: 4-Chloromercuribenzenesulfonate; Ascorbic Acid; Bacterial Infections; Blood Bactericidal Activity; Child; Cyclooxygenase Inhibitors; Cytochalasin B; Ethylmaleimide; Granulomatous Disease, Chronic; Humans; Indomethacin; Luminescent Measurements; Male; Middle Aged; Neutrophils; Peroxidase; Phenothiazines; Phenylbutazone

1982
Effects of ascorbic acid on neutrophil function. Studies on normal and chronic granulomatous disease neutrophils.
    Acta vitaminologica et enzymologica, 1982, Volume: 4, Issue:1-2

    Ascorbic acid is able to stimulate neutrophil oxidative metabolism in normal neutrophils, as well as other several functions of these cells, either in the normal state or in the defective one. In the present study, we have investigated the effects of ascorbic acid on the hexose monophosphate shunt (HMPS) and on the bactericidal activity of neutrophils from Chronic Granulomatous Disease (CGD) patients. Furthermore, we have investigated the effects of ascorbic acid on the antibody dependent cell cytotoxicity (ADCC) of normal neutrophils. Ascorbic acid in vitro was able to significantly improve both HMPS activity and bacterial killing of CGD neutrophils. Its prolonged administration to such patients led to consistent clinical improvement, possibly related to the enhancement of chemotaxis, although the effects on HMPS and bacterial killing seen in vitro could not be confirmed. Ascorbic acid was also able to interfere with neutrophil ADCC with different results depending on its concentration and the experimental conditions.

    Topics: Antibody-Dependent Cell Cytotoxicity; Ascorbic Acid; Blood Bactericidal Activity; Chemotaxis, Leukocyte; Granulomatous Disease, Chronic; Hexosephosphates; Humans; Neutrophils

1982
Assessment of oral ascorbate in three children with chronic granulomatous disease and defective neutrophil motility over a 2-year period.
    Clinical and experimental immunology, 1981, Volume: 43, Issue:1

    Two brothers and their sister with chronic granulomatous disease, elevated levels of serum IgE and defective neutrophil motility were treated with a single oral daily dose of 1 g sodium ascorbate as a supplement to prophylactic trimethoprim--sulphamethoxazole therapy for 2 years. Laboratory tests of neutrophil functions were performed prior to ascorbate therapy and repeated at 1-monthly intervals for 6 months and at 6-monthly intervals thereafter. Introduction of ascorbate to the therapeutic regimen was accompanied by slight increases in neutrophil hexose monophosphate shunt activity and staphylocidal activity and good improvement of neutrophil motility in all three children. The improved staphylocidal activity was not due to ascorbate-mediated inhibition of neutrophil or serum catalase activities or to detectable increases in superoxide and H2O2 production or activity of the MPO/H2O2/halide system. Both male children have remained free from obvious infection since ascorbate was added to their therapeutic regimen; their sister has experienced one urinary tract infection during a period when treatment with prophylactic co-trimoxazole and ascorbate was inadvertently stopped. All three children have gained weight.

    Topics: Ascorbic Acid; Blood Bactericidal Activity; Catalase; Chemotaxis, Leukocyte; Child; Child, Preschool; Female; Granulomatous Disease, Chronic; Humans; Hydrogen Peroxide; Immunoglobulin E; Male; Neutrophils; Superoxides

1981
Oxidation of glucosamine by human polymorphonuclear leukocytes.
    Inflammation, 1981, Volume: 5, Issue:1

    When exposed to a phagocytic stimulus (opsonized zymosan), human polymorphonuclear leukocytes (PMNs) produced 14CO2 from [1-14C]glucosamine at a rate 10-25% of that produced from glucose under the same conditions. The production of CO2 from glucosamine by intact PMNs was inhibited by glucose and dependent upon activation of the hexosemonophosphate shunt (HMPS). However, the metabolic pathways for the oxidation of glucose and glucosamine by PMNs are not identical. This is suggested by the fact that glucose-6-phosphate dehydrogenase, the initiating enzyme for the HMPS, did not utilize glucosamine-6-phosphate as a substrate. In addition, glucosamine was not oxidized by sonically disrupted PMNs whereas oxidation of glucose by the same preparation was increased sevenfold over intact cells. Taken together, the data suggest that PMNs oxidize glucosamine by converting it to a compound compatible with the enzymes of the HMPS. This conversion requires intact PMNs and/or an as yet unidentified cofactor.

    Topics: Ascorbic Acid; Carbon Dioxide; Decarboxylation; Fructosephosphates; Glucosamine; Glucose; Glucose-6-Phosphate; Glucosephosphates; Granulomatous Disease, Chronic; Hexosephosphates; Humans; Neutrophils; Oxygen Consumption; Phagocytosis; Sonication

1981
Repeated staphylococcal pyoderma in two siblings with defective neutrophil bacterial killing.
    Dermatologica, 1980, Volume: 160, Issue:2

    2 children with undue susceptibility to skin infections and isolated defective neutrophil bacterial killing are described. Since the NBT-reducing capabilities of granulocytes were normal, a mild form of chronic granulomatous disease was excluded. Ascorbic acid was effective in delaying and eventually suppressing infectious episodes.

    Topics: Ascorbic Acid; Blood Bactericidal Activity; Chemotaxis, Leukocyte; Child; Female; Granulomatous Disease, Chronic; Humans; Lymphocytes; Male; Neutrophils; Pyoderma; Recurrence; Staphylococcal Infections

1980
Effects of ascorbate on leucocytes: Part IV. Increased neutrophil function and clinical improvement after oral ascorbate in 2 patients with chronic granulomatous disease.
    South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde, 1979, Sep-01, Volume: 56, Issue:12

    A brother and sister with chronic granulomatous disease (CGD) of the autosomal recessive type, and with markedly defective neutrophil motility and elevated serum IgE levels, were treated with a single oral daily dose of 1 g ascorbate for 6 months. Neutrophil function and serum IgE levels were measured repeatedly at approximately monthly intervals. Both children also received prophylactic antibiotics which were always stopped 1 week prior to testing of immune function. Ascorbate treatment was accompanied by significantly increased neutrophil motility and post-phagocytic metabolic activity, and a reduction in serum IgE levels. Enhanced neutrophil function correlated with clinical improvement. Both children have remained free of infection since ascorbate was added to their regimen and have gained weight.

    Topics: Ascorbic Acid; Blood Proteins; Cell Movement; Chemotaxis, Leukocyte; Child; Child, Preschool; Female; Granulomatous Disease, Chronic; Hexosephosphates; Humans; Immunoglobulin E; Immunoglobulins; Iodine; Male; Neutrophils; Nitroblue Tetrazolium; Protein Binding

1979
Oxidative bactericidal mechanisms of polymorphonuclear leukocytes.
    The Journal of infectious diseases, 1975, Volume: 131, Issue:3

    Topics: Ascorbic Acid; Blood Bactericidal Activity; D-Amino-Acid Oxidase; Dihydrolipoamide Dehydrogenase; Granulomatous Disease, Chronic; Humans; NADP; Neutrophils; Nitroblue Tetrazolium; Oxidation-Reduction; Oxygen Consumption; Phagocytosis; Superoxide Dismutase; Superoxides

1975
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