ascorbic-acid and Fascioliasis

The aim of the present study was to investigate the effect of triclabendazole (CAS 68786-66-3) therapy alone or in combination with ascorbic acid (vitamin C, CAS 50-81-7) and tocofersolan (vitamin E, CAS 30999-06-5), in Fasciola hepatica patients, on Lipo-peroxidation (LPO) and blood antioxidant capacity. 32 Fasciola hepatica patients were divided into two groups (16 acute and 16 chronic). Each group was divided into two subgroups of 8 patients each. One subgroup was given two consecutive oral doses each of 10 mg/kg body weight of triclabendazole suspension and the other received vitamin C (1000 mg/day) and vitamin E (600 mg/day) for two months, together with the same dose of triclabendazole given to the first subgroup. Ten healthy subjects served as controls. The results revealed a significant increase in serum and erythrocyte lipid peroxide levels and a significant decrease in glutathione levels as well as in glutathione peroxidase (GPX) and superoxide dismutase (SOD) activities in all study groups compared to their corresponding control values. After triclabendazole treatment, pronounced improvements in all studied parameters were observed which could be attributed to the fasciolicidal effect of the drug. The significant improvement of SOD and GPX activities and in lipid peroxide levels after vitamins supplementation as compared to their corresponding values after treatment with triclabendazole alone could be explained on the basis of the potent action of these vitamins in protection against oxidative damage.

Topics: Acute Disease; Adolescent; Adult; Anthelmintics; Antioxidants; Ascorbic Acid; Benzimidazoles; Chronic Disease; Drug Combinations; Erythrocytes; Fascioliasis; Female; Glutathione; Glutathione Peroxidase; Humans; Lipid Peroxides; Liver Function Tests; Male; Superoxide Dismutase; Triclabendazole; Vitamin E

The aim of this study was to assess the effects of natural distomatosis infections on sheep liver malondialdehyde (MDA) concentration, activities of enzymatic antioxidants (glutathione peroxidase (GPx), superoxide dismutase (Cu, Zn-SOD), catalase (CAT)) and concentrations of non-enzymatic antioxidants (reduced glutathione (GSH), vitamin C, and beta-carotene). Eighteen Akkaraman sheep naturally infected with Fasciola sp and Dicrocoelium dentriticum (D. dentriticum) and ten healthy Akkaraman sheep were included in the study Liver samples for the analysis of MDA, GPx, Cu, Zn-SOD, CAT, GSH, vitamin C, and beta-carotene and blood samples for the measurement of alanine aminotransferase and aspartate aminotransferase were collected immediately after sheep in the two groups were slaughtered. The concentration of MDA and activity of GPx in the group with distomatosis were higher than in the control group (P < 0.001). However, the Cu, Zn-SOD, CAT activities and the GSH, vitamin C concentrations in the infected group were significantly lower than in the control group (P < 0.001). The serum beta-carotene was not found to be statistically different in the two groups (P > 0.05). ALT and AST serum activities of the group with distomatosis were significantly higher in comparison to the control group (P < 0.001). In this study it was demonstrated that lipid peroxidation increased and activities or/and concentrations of antioxidant compounds were significantly changed in the liver of sheep with distomatosis.

Topics: Alanine Transaminase; Animals; Antioxidants; Ascorbic Acid; Aspartate Aminotransferases; beta Carotene; Catalase; Dicrocoeliasis; Dicrocoelium; Fasciola; Fasciola hepatica; Fascioliasis; Glutathione; Glutathione Peroxidase; Lipid Peroxidation; Liver; Malondialdehyde; Sheep; Sheep Diseases; Superoxide Dismutase

Fasciola hepatica infection is accompanied by increased formation of reactive oxygen species. The aim of this study was to analyze antioxidative properties of rat serum in the course of fasciolosis. Wistar rats were infected per os with 30 metacercariae of F. hepatica. Activities of antioxidant enzymes and concentrations of non-enzymatic antioxidants in serum were determined at 4, 7, and 10 weeks post-infection (wpi). Activity of superoxide dismutase (Cu, Zn-SOD) significantly decreased (by 35% during the migratory phase, by 40 and 23% at 7 and 10 wpi, respectively), while glutathione reductase activity significantly increased (by 62, 65, and 41%, at 4, 7, and 10 wpi, respectively). No significant changes were found in the activity of glutathione peroxidase. Significant decreases in concentrations of reduced glutathione, vitamins C, E, and A were observed, particularly during the migratory phase of fasciolosis (at 4 wpi). These changes were accompanied by enhancement of lipid peroxidation processes as evidenced by increased levels of malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE). Concentrations of MDA and 4-HNE at 4 wpi increased by 38% and by 59%. MDA increased by 51% at 7 wpi and by 79% at 10 wpi, while 4-HNE increased by 87 and 118%, respectively. The results indicate that fasciolosis is associated with enhanced oxidative reactions and reduced antioxidant defense capability of rat serum.

Topics: Aldehydes; Animals; Antioxidants; Ascorbic Acid; Bile Ducts; Fasciola hepatica; Fascioliasis; Glutathione; Glutathione Peroxidase; Glutathione Reductase; Lipid Peroxidation; Liver; Male; Malondialdehyde; Rats; Rats, Wistar; Serum; Superoxide Dismutase; Vitamin A; Vitamin E

Three groups of sheep, one control (A) and two infected with Fasciola hepatica metacercariae (B&C), were investigated. Each animal of group B was orally dosed with about 270 metacercariae and each sheep in group C received two such doses administered 14 days apart. In both infected groups, plasma ascorbic acid levels progressively decreased after exposure reaching a minimum by week 9, and then slightly increased during the subsequent five weeks. The decrease in ascorbic acid concentrations was mainly related to impaired synthesis as a result of the hepatic damage caused by the flukes.

Topics: Animals; Ascorbic Acid; Biliary Tract; Blood Glucose; Fasciola hepatica; Fascioliasis; Liver; Serum Albumin; Serum Globulins; Sheep; Sheep Diseases

Topics: Animals; Ascorbic Acid; Fasciola hepatica; Fascioliasis; Kidney; Liver; Liver Glycogen; Rats; Spleen

Six sheep were infected with about 520 Fasciola hepatica metacercariae. Plasma ascorbic acid decreased after infection attaining minimum values by week 10 and subsequently increased. Plasma iron concentrations were elevated during the first 6 weeks of exposure and then decreased till week 10; the levels approached pre-infection values at weeks 11-13 and again dropped. The iron-binding capacity rose during infection especially between weeks 13 and 19.

Topics: Animals; Ascorbic Acid; Fasciola hepatica; Fascioliasis; Iron; Sheep; Sheep Diseases