ascorbic-acid and Eye-Injuries

Topics: Animals; Antioxidants; Ascorbic Acid; Cells, Cultured; Cornea; Corneal Injuries; Eye Injuries; Fibroblasts; Filtering Surgery; Glaucoma; Humans; Wound Healing

Conventional analytical techniques, which have been developed for high sensitivity and selectivity for the detection and quantification of relevant biomarkers, may not be as suitable for medical diagnosis in resource scarce environments as compared to point-of-care devices (POC). We have developed a new reactive sensing material which contains ionic gold entrapped within an agarose gel scaffold for POC quantification of ascorbic acid (AA) in tear fluid. Pathologically elevated concentration of AA in human tear fluid can serve as a biomarker for full-thickness injuries to the ocular surface, which are a medical emergency. This reactive sensing material will undergo colorimetric changes, quantitatively dependent on endogenous bio-reductants that are applied, as the entrapped ionic gold is reduced to form plasmonic nanoparticles. The capacity for this reactive material to function as a plasmonically driven biosensor, called 'OjoGel' (ojo-eye), was demonstrated with the endogenous reducing agent, AA. Through applications of AA of varied concentrations to the OjoGel, we demonstrated a quantitative colorimetric relationship between red (R) hexadecimal values and concentrations of AA in said treatments. This colorimetric relationship is directly resultant of plasmonic gold nanoparticle formation within the OjoGel scaffold. Using a commercially available mobile phone-based Pixel Picker

Topics: Ascorbic Acid; Biosensing Techniques; Colorimetry; Eye Injuries; Gels; Gold; Humans; Tears

Topics: Alkalies; Ascorbic Acid; Burns, Chemical; Corneal Transplantation; Eye Injuries; Heparin; Humans

Topics: Ascorbic Acid; Eye Injuries; Glycerol; Humans; Hyphema

Topics: Ascorbic Acid; Burns, Chemical; Eye Injuries; Female; Humans; Potassium Permanganate

Topics: Albumins; Alpha-Globulins; Animals; Aqueous Humor; Ascorbic Acid; Beta-Globulins; Cattle; Densitometry; Electrophoresis, Polyacrylamide Gel; Epinephrine; Eye; Eye Injuries; Eye Proteins; gamma-Globulins; Humans; In Vitro Techniques; Light; Protein Binding; Radiation Effects; Radiation Injuries; Tartrates

Topics: Adrenal Cortex Hormones; Ascorbic Acid; Cornea; Eye Injuries; Humans; Wound Healing

Topics: Anti-Infective Agents, Local; Ascorbic Acid; Chelating Agents; Eye Injuries; Humans; Potassium Permanganate; Vitamins

Topics: Alkalies; Ascorbic Acid; Eye Injuries; Humans; Vitamins