ascorbic-acid and Escherichia-coli-Infections
ascorbic-acid has been researched along with Escherichia-coli-Infections* in 20 studies
Reviews
1 review(s) available for ascorbic-acid and Escherichia-coli-Infections
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UTIs in patients with neurogenic bladder.
Urinary tract infections (UTI) remain one of the most prevalent and frustrating morbidities for neurogenic bladder patients, and death attributed to urosepsis in the spinal cord injury (SCI) patient is higher when compared to the general population. Risk factors include urinary stasis, high bladder pressures, bladder stones, and catheter use. While classic symptoms of UTI include dysuria, increased frequency and urgency, neurogenic bladder patients present differently with increased spasticity, autonomic dysreflexia, urinary incontinence, and vague pains. Multiple modalities have been assessed for prevention including catheter type, oral supplements, bladder irrigation, detrusor injections and prophylactic antimicrobials. Of these, bladder inoculation with E. coli HU2117, irrigation with iAluRil(®), detrusor injections, and weekly prophylaxis with alternating antibiotics appear to have a positive reduction in UTI but require further study. Ultimately, treatment for symptomatic UTI should account for the varied flora and possible antibiotic resistances including relying on urine cultures to guide antibiotic therapy. Topics: Administration, Intravesical; Anti-Bacterial Agents; Anti-Infective Agents, Urinary; Antibiotic Prophylaxis; Antioxidants; Ascorbic Acid; Botulinum Toxins, Type A; Catheter-Related Infections; Escherichia coli; Escherichia coli Infections; Humans; Immunotherapy, Active; Mannose; Methenamine; Multiple Sclerosis; Neuromuscular Agents; Proanthocyanidins; Probiotics; Recurrence; Spinal Cord Injuries; Urinary Bladder, Neurogenic; Urinary Catheterization; Urinary Catheters; Urinary Tract Infections | 2014 |
Trials
1 trial(s) available for ascorbic-acid and Escherichia-coli-Infections
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Ascorbic Acid Differentially Affects Stress Response and Resistance to Colibacillosis in Turkeys from Genetic Lines Differing in Growth Rate.
Genetic selection for fast growth can affect the ability of male turkeys to cope with stressors, resulting in decreased immunity to opportunistic bacterial infection. The purpose of the current study was to compare the effects of ascorbic acid (AA) on the stress response and resistance to Escherichia coli challenge of birds selected for increased 16-wk body weight (BW; F-line) with their random-bred parent line (RBC2). Male turkeys were raised in duplicate floor pens in a two line×two AA treatment×two stress challenge (SC) design. At 5 wk of age, AA (1200 ppm) was provided in drinking water for a 24-hr period, during which all birds were weighed. After AA treatment, the SC group was subjected to a transport stress protocol. Six hours after the start of transport, SC birds were also inoculated in the thoracic air sac with 1×10(4) colony-forming units of E. coli. The following morning four birds from each pen were bled, and all birds were weighed and necropsied 2 days later. BW and gain after SC were decreased in the F-line but not the RBC2 line, and there were no AA effects on BW. The weight of the bursa of Fabricius relative to BW was higher in the RBC2 line than in the F-line, was decreased by SC, and was not affected by AA. The heterophil∶lymphocyte ratio was higher in the SC F-line as compared to the SC RBC2 and was decreased by AA only in the SC F-line. Corticosterone (C) levels were increased by SC only in the F-line, and AA decreased C levels only in the RBC2 line. Airsacculitis scores were increased in the F-line SC birds. The challenge strain of E. coli was only detected in the air sac and liver of the AA-treated F-line SC birds and in the liver of the no-AA F-line birds. These results suggest that SC at 5 wk of age had a more deleterious effect on the fast-growing F-line than on its parent line and that AA may have increased susceptibility to colibacillosis in the SC F-line birds. Topics: Animals; Ascorbic Acid; Dietary Supplements; Escherichia coli Infections; Male; Poultry Diseases; Stress, Physiological; Turkeys | 2015 |
Other Studies
18 other study(ies) available for ascorbic-acid and Escherichia-coli-Infections
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Antibacterial effect of vitamin C against uropathogenic E. coli in vitro and in vivo.
Resistance to antibiotics has increased steadily over time, thus there is a pressing need for safer alternatives to antibiotics. Current study aims to evaluate the influence of vitamin C as an antibacterial and anti-biofilm agent against uropathogenic E. coli (UPEC) strains. The expression of beta-lactamases and biofilm encoding genes among E. coli isolates before and after treating the isolates with sub MIC of vitamin C was analyzed by Real-time PCR. The in vivo assessment of the antibacterial and anti-biofilm effects of vitamin C against uropathogenic E. coli strains was done using a urinary tract infection (UTI) rat model.. The effective concentration of vitamin C that could inhibit the growth of most study isolates (70%) was 1.25 mg/ml. Vitamin C showed a synergistic effect with most of the studied antibiotics; no antagonistic effect was detected at all. Vitamin C showed an excellent anti-biofilm effect against studied isolates, where 43 biofilm-producing isolates were converted to non-biofilm at a concentration of 0.312 mg/ml. The expression levels of most studied genes were down-regulated after treatment of E. coli isolates with vitamin C. In vivo assessment of vitamin C in treating UTIs showed that vitamin C has a rapid curative effect as the comparable antibiotic. Administration of both vitamin C and nitrofurantoin at a lower dose for treatment of UTI in rats had a better effect.. Vitamin C as an antibacterial and anti-biofilm agent either alone or in combination with antibiotics could markedly improve UTI in experimental rats. Topics: Animals; Anti-Bacterial Agents; Ascorbic Acid; Escherichia coli Infections; Nitrofurantoin; Rats; Urinary Tract Infections; Uropathogenic Escherichia coli; Vitamins | 2023 |
Enteropathogenic Escherichia coli Infection Inhibits Intestinal Ascorbic Acid Uptake via Dysregulation of Its Transporter Expression.
Enteropathogenic Escherichia coli (EPEC) infection causes prolonged, watery diarrhea leading to morbidity and mortality. Although EPEC infection impacts nutrient transporter function and expression in intestinal epithelial cells, the effects of EPEC infection on intestinal absorption of ascorbic acid (AA) have not yet been investigated.. To investigate the effect of EPEC infection on intestinal AA uptake process and expression of both AA transporters.. We used two experimental models: human-derived intestinal epithelial Caco-2 cells and mice.. EPEC (WT) as well as ΔespF and ΔespG/G2 mutant-infected Caco-2 cells showed markedly inhibited AA uptake, while other mutants (ΔescN, ΔespA, ΔespB, and ΔespD) did not affect AA uptake. Infection also reduced protein and mRNA expression levels for both hSVCT1 and hSVCT2. EPEC-infected mice showed marked inhibitory effect on AA uptake and decreased protein and mRNA expression levels for both mSVCT1 and mSVCT2 in jejunum and colon. MicroRNA regulators of SVCT1 and SVCT2 (miR103a, miR141, and miR200a) were upregulated significantly upon EPEC infection in both Caco-2 and mouse jejunum and colon. In addition, expression of the accessory protein glyoxalate reductase/hydroxypyruvate reductase (GRHPR), which regulates SVCT1 function, was markedly decreased by EPEC infection in both models.. These findings suggest that EPEC infection causes inhibition in AA uptake through a multifactorial dysregulation of SVCT1 and SVCT2 expression in intestinal epithelial cells. Topics: Animals; Ascorbic Acid; Biological Transport; Caco-2 Cells; Enteropathogenic Escherichia coli; Escherichia coli Infections; Escherichia coli Proteins; Gene Expression Regulation; Humans; Intestinal Mucosa; Mice; Mutation; RNA, Messenger; Sodium-Coupled Vitamin C Transporters | 2021 |
Reversal of the Pathophysiological Responses to Gram-Negative Sepsis by Megadose Vitamin C.
Oxidative stress appears to initiate organ failure in sepsis, justifying treatment with antioxidants such as vitamin C at megadoses. We have therefore investigated the safety and efficacy of megadose sodium ascorbate in sepsis.. Interventional study.. Research Institute.. Adult Merino ewes.. Sheep were instrumented with pulmonary and renal artery flow-probes, and laser-Doppler and oxygen-sensing probes in the kidney. Conscious sheep received an infusion of live Escherichia coli for 31 hours. At 23.5 hours of sepsis, sheep received fluid resuscitation (30 mL/kg, Hartmann solution) and were randomized to IV sodium ascorbate (0.5 g/kg over 0.5 hr + 0.5 g/kg/hr for 6.5 hr; n = 5) or vehicle (n = 5). Norepinephrine was titrated to restore mean arterial pressure to baseline values (~80 mm Hg).. Sepsis-induced fever (41.4 ± 0.2°C; mean ± se), tachycardia (141 ± 2 beats/min), and a marked deterioration in clinical condition in all cases. Mean arterial pressure (86 ± 1 to 67 ± 2 mm Hg), arterial Po2 (102.1 ± 3.3 to 80.5 ± 3.4 mm Hg), and renal medullary tissue Po2 (41 ± 5 to 24 ± 2 mm Hg) decreased, and plasma creatinine doubled (71 ± 2 to 144 ± 15 µmol/L) (all p < 0.01). Direct observation indicated that in all animals, sodium ascorbate dramatically improved the clinical state, from malaise and lethargy to a responsive, alert state within 3 hours. Body temperature (39.3 ± 0.3°C), heart rate (99.7 ± 3 beats/min), and plasma creatinine (32.6 ± 5.8 µmol/L) all decreased. Arterial (96.5 ± 2.5 mm Hg) and renal medullary Po2 (48 ± 5 mm Hg) increased. The norepinephrine dose was decreased, to zero in four of five sheep, whereas mean arterial pressure increased (to 83 ± 2 mm Hg). We confirmed these physiologic findings in a coronavirus disease 2019 patient with shock by compassionate use of 60 g of sodium ascorbate over 7 hours.. IV megadose sodium ascorbate reversed the pathophysiological and behavioral responses to Gram-negative sepsis without adverse side effects. Clinical studies are required to determine if such a dose has similar benefits in septic patients. Topics: Animals; Antioxidants; Ascorbic Acid; Bacteremia; Disease Models, Animal; Dose-Response Relationship, Drug; Escherichia coli Infections; Sepsis; Sheep | 2021 |
The possible neurobehavioral protective effects of natural antioxidant against phototoxicity attenuation of antimicrobial quinolone group in rats.
The fluoroquinolones absorb light in the 320 to 330 nm ultraviolet A (UV-A) wavelength and produce reactive oxygen species (ROS) such as superoxide anion, hydroxyl radical, and hydrogen peroxide; thus, the photodynamic generation of ROS may be the basis of phototoxicity of quinolones in human beings and animals. This study aimed to evaluate the damaging effects of UV-A radiation at different periods of exposure on rats' brains administered with ciprofloxacin. Ciprofloxacin administration in UV-A exposed animals exaggerated the brain-oxidative stress biomarkers and decreased the locomotor activity. Exposure of rats to UV-A for 60 minutes induced a significant increase of malondialdehyde (MDA), myeloperoxidase (MPO), and a decrease in the values of superoxide dismutase (SOD), glutathione (GSH) compared to a normal one; these changes were UV-A exposure time-dependent. However, the administration of vitamin C to the UV-60-treated group decreased the values of MDA, MPO, and shifted the values of SOD, GSH toward the normal values. Vitamin C, probably due to its strong antioxidant properties, could improve and partially counteract the toxic effect of UV-A on oxidative stress parameters and prevent the damage in rat's brain tissues. Topics: Animals; Anti-Bacterial Agents; Antioxidants; Ascorbic Acid; Behavior, Animal; Cerebrum; Ciprofloxacin; Dermatitis, Phototoxic; Escherichia coli; Escherichia coli Infections; Glutathione; Locomotion; Male; Malondialdehyde; Oxidative Stress; Peroxidase; Rats; Reactive Oxygen Species; Signal Transduction; Superoxide Dismutase; Ultraviolet Rays | 2020 |
Enterotoxigenic Escherichia coli heat labile enterotoxin inhibits intestinal ascorbic acid uptake via a cAMP-dependent NF-κB-mediated pathway.
Vitamin C is an antioxidant and acts as a cofactor for many enzymatic reactions. Humans obtain vitamin C from dietary sources via intestinal absorption, a process that involves the sodium-dependent vitamin C transporters-1 and -2 (SVCT1 and SVCT2). Enterotoxigenic Escherichia coli (ETEC) infection impacts intestinal absorption/secretory functions, but nothing is known about its effect on ascorbic acid (AA) uptake. Here we demonstrate that infection of Caco-2 cells with ETEC led to a significant inhibition in intestinal AA uptake. This inhibition was associated with a marked reduction in hSVCT1 and hSVCT2 protein, mRNA, and heterogeneous nuclear RNA (hnRNA) expression levels as well as significant inhibition in the activity of both the SLC23A1 and SLC23A2 promoters. Similarly, exposure of mice to ETEC led to a significant inhibition in intestinal AA uptake and reduction in mSVCT1 and mSVCT2 protein, mRNA, and hnRNA expression levels. Inhibition was caused by the action of heat labile enterotoxin (LT), since infecting Caco-2 cells with LT-deficient ETEC (ΔLT) failed to impact AA uptake. Because LT activates adenylate cyclase, we also examined the effect of dibutyryl-cAMP in AA uptake by Caco-2 cells and observed a significant inhibition. Furthermore, treating the cells with celastrol, a specific NF-κB inhibitor, significantly blocked the inhibition of AA uptake caused by ETEC infection. Together, these data demonstrate that ETEC infection impairs intestinal AA uptake through a cAMP-dependent NF-κB-mediated pathway that regulates both SLC23A1 and SLC23A2 transcription. NEW & NOTEWORTHY Our findings demonstrate that heat-labile enterotoxin produced by enterotoxigenic Escherichia coli inhibits AA uptake in intestinal epithelial cells and mouse intestine. This effect is mediated through transcriptional repression of SLC23A1 (SVCT1) and SLC23A2 (SVCT2) via a cAMP-dependent NF-κB signaling pathway. Topics: Animals; Ascorbic Acid; Biological Transport; Caco-2 Cells; Enterotoxigenic Escherichia coli; Enterotoxins; Epithelial Cells; Escherichia coli Infections; Humans; Intestinal Mucosa; Intestines; NF-kappa B; Sodium-Coupled Vitamin C Transporters; Vitamins | 2019 |
Amelioration of Escherichia coli-induced endometritis with ascorbic acid in non-pregnant mouse models.
Infection-induced endometritis is associated with infertility. The outcome with oral antibiotics remains poor. This study therefore investigates the role of ascorbic acid in resolving endometritis.. Animals with established oestrus cycles were inoculated with Escherichia coli. Two days post-inoculation, the animals were administered ascorbic acid (10, 100 and 1000 mg/kg) and amoxicillin (500 mg/kg) for 5 days. Other groups included water only and E. coli inoculated with no treatment. Body temperatures, weights and vaginal cytology were examined. On the sixth day, after anaesthesia, blood samples were obtained for haematological analysis. Uterine organs were weighed, ex-vivo functionality analysed and histopathological analysis performed.. Ascorbic acid (AA) (100 and 1000 mg/kg) regularized the cycle of the endometritic animals comparable to amoxicillin. AA (1000 mg/kg) and amoxicillin, significantly decreased (P < .05) the endometritis-induced increase in uterine weights, restored the endometrial architecture and significantly (P < .05) normalized uterine contractions to control values. Improved haematological profiles were additionally observed on treatment with ascorbic acid (100 and 1000 mg/kg).. AA compared favourably with amoxicillin in endometritis management, suggesting that AA can be used in the management of infection-induced endometritis, normal cycling and normal uterine function. Topics: Amoxicillin; Animals; Anti-Bacterial Agents; Ascorbic Acid; Disease Models, Animal; Endometritis; Endometrium; Escherichia coli; Escherichia coli Infections; Female; Hematologic Tests; Humans; Mice; Mice, Inbred Strains; Uterus | 2018 |
Inactivation of Escherichia coli O157:H7, Salmonella typhimurium, and Listeria monocytogenes on cherry tomatoes and oranges by superheated steam.
This study was performed to compare the effectiveness of saturated steam and superheated steam for the inactivation of Escherichia coli O157:H7, Salmonella typhimurium, and Listeria monocytogenes on the surface of cherry tomatoes and oranges. It also determined the effect of the steam processes on the color, texture, Vitamin C content, and antioxidant capacity and changes in these parameters during chilled storage. Cherry tomatoes and oranges inoculated with the three foodborne pathogens were treated with saturated steam at 100 °C and superheated steam at 125, 150, 175, and 200 °C for various time intervals. After the cherry tomatoes and oranges were exposed to superheated steam at 200 °C for 3 or 20 s, all tested pathogens were reduced to below the detection limit (1 or 1.7 log, respectively) without significant changes in color, texture, vitamin C content, and antioxidant capacity (P > .05) at 4 °C for up to 9 days. Our results suggest that superheated steam treatment can be effective at decreasing pathogen populations when compared to saturated steam, without significant quality deterioration, and thus, this technique demonstrates great potential to improve the microbial safety of fresh produce. Topics: Antioxidants; Ascorbic Acid; Citrus sinensis; Color; Escherichia coli; Escherichia coli Infections; Food Microbiology; Foodborne Diseases; Fruit; Hot Temperature; Listeria monocytogenes; Listeriosis; Microbial Viability; Pasteurization; Salmonella Food Poisoning; Salmonella typhimurium; Solanum lycopersicum; Steam; Time Factors | 2018 |
Synthesis of monodispersed silver nanoparticles using Hibiscus cannabinus leaf extract and its antimicrobial activity.
Synthesis of silver nanoparticles using leaf extract of Hibiscus cannabinus has been investigated. The influences of different concentration of H. cannabinus leaf extract, different metal ion concentration and different reaction time on the above cases on the synthesis of nanoparticles were evaluated. The synthesized nanoparticles were characterized using UV-vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and Transmission Electron Microscopy (TEM). The prepared silver nanoparticles were monodispersed, spherical in shape with the average particle size of 9 nm and shows surface plasmon peak at 446 nm. The study also reveals that the ascorbic acid present in H. cannabinus leaf extract has been used as reducing agent. The prepared silver nanoparticle shows good antimicrobial activity against Escherichia coli, Proteus mirabilis and Shigella flexneri. Topics: Anti-Infective Agents; Ascorbic Acid; Dysentery, Bacillary; Escherichia coli; Escherichia coli Infections; Hibiscus; Humans; Nanoparticles; Oxidation-Reduction; Plant Extracts; Plant Leaves; Proteus Infections; Proteus mirabilis; Shigella flexneri; Silver; Spectrum Analysis | 2013 |
Intravesical nitric oxide delivery for prevention of catheter-associated urinary tract infections.
The use of indwelling urinary catheters is a major risk factor for urinary tract infection; and despite the availability of numerous preventive regimens, this condition is still extremely common. In earlier studies we have demonstrated the inhibitory effects of nitrite and ascorbic acid on bacterial growth in urine. When combined, these compounds generate antibacterial reactive nitrogen species, including the gas nitric oxide. We have now tested in a laboratory model of the urinary bladder whether filling of the catheter retention balloon with nitrite and ascorbic acid would generate measurable amounts of NO outside the membrane and whether this would affect bacterial growth in the surrounding urine. Two strains of Escherichia coli, one strain isolated from a patient (U1106024) and one reference strain (ATCC 25922), were tested. Nitric oxide gas was generated in the silicone balloon and readily diffused into the urine. When control catheters with ascorbic acid but without nitrite were used, bacterial counts increased from 9.0 x 10(5) to 2.0 x10(8) CFU/ml (strain U1106024) and from 2.5 x 10(6) to 2.7 x 10(8) CFU/ml (strain ATCC 25922) after 24 h. In contrast, in test catheters with ascorbic acid and nitrite, both strains tested were effectively killed. The NO donor {DETA NONOate, (Z)-1-[N-(2-aminoethyl)-N-(2-ammonioethyl)amino]diazen-1-ium-1,2-diolate} also showed antibacterial activity in the same model, thereby supporting a central role of NO in achieving the observed effects. Future clinical trials will reveal whether this novel approach for the intravesical delivery of an antibacterial gas could be used to prevent catheter-associated infections. Topics: Administration, Intravesical; Anti-Bacterial Agents; Ascorbic Acid; Catheters, Indwelling; Colony Count, Microbial; Escherichia coli; Escherichia coli Infections; Humans; Laboratories; Models, Anatomic; Nitric Oxide; Nitrites; Urinary Bladder; Urinary Catheterization; Urinary Tract Infections; Urine | 2005 |
Changes in vitamin C concentrations in plasma and milk from dairy cows after an intramammary infusion of Escherichia coli.
Plasma and milk concentrations of ascorbic acid and dehydro-L-ascorbic acid (DHAA) were measured before and after 21 Holstein cows (approximately 26 DIM) were given an intramammary infusion of Escherichia coli. Blood, milk from the unchallenged quarters, and milk from the challenged gland were sampled immediately before challenge (d 0) and 24 h and 7 d postchallenge. Plasma vitamin C (ascorbic acid + DHAA) concentrations decreased 39%, and concentrations of vitamin C and ascorbic acid in milk from the challenged quarter decreased 52 and 62%, respectively, in samples taken 24 h postchallenge. No change was observed in vitamin C concentrations in milk from unchallenged quarters. The concentration of DHAA in milk from challenged quarters increased 67% 24 h postchallenge. The duration of clinical mastitis, peak body temperature, number of colony-forming units of E. coli isolated from the infected gland, and loss in milk yield were associated with a change in concentration of vitamin C in milk from the challenged quarter. Increased severity of clinical signs was associated with large decreases in concentration of vitamin C in milk from the challenged quarter. Similar, but statistically weaker, relationships were observed for changes in plasma vitamin C concentrations. Topics: Animals; Ascorbic Acid; Cattle; Colony Count, Microbial; Dehydroascorbic Acid; Escherichia coli Infections; Female; Mastitis, Bovine; Milk | 2004 |
Inflammation-induced vasoconstrictor hyporeactivity is caused by oxidative stress.
We sought to determine the role of oxidative stress in the development of vascular dysfunction in inflammation.. Hyporeactivity to catecholamines and other vasoconstrictors is present in acute inflammation. Because oxidative stress plays a significant role in inflammation, impaired responsiveness may be overcome by anti-oxidants.. In randomized, double-blind, cross-over studies, forearm blood flow (FBF) responses to norepinephrine (NE), angiotensin II (ANG II), and vasopressin (VP) were assessed before and 4 h after induction of systemic inflammation by low doses of Escherichia coli endotoxin (lipopolysaccharide [LPS], 20 IU/kg intravenously) or after placebo in healthy volunteers. Furthermore, the effect of intra-arterial vitamin C (24 mg/min) or placebo on NE-induced or ANG II-induced vasoconstriction was studied after LPS.. Administration of LPS caused systemic and forearm vasodilation, increased white blood cell count, elevated body temperature, and reduced vitamin C plasma concentrations. Lipopolysaccharide decreased the responses of FBF to NE by 59%, to ANG II by 25%, and to VP by 51% (n = 9, p < 0.05, all effects). Co-administration of vitamin C completely restored the response to NE and to ANG II, which was comparable to that observed under baseline conditions (n = 8).. E. coli-endotoxemia reduces FBF responsiveness to vasoconstrictors. The hyporeactivity can be corrected by high doses of vitamin C, suggesting that oxidative stress may represent an important target for inflammation-induced impaired vascular function. Topics: Adult; Angiotensin II; Antioxidants; Ascorbic Acid; Endotoxemia; Escherichia coli Infections; Humans; Inflammation; Male; Oxidative Stress; Regional Blood Flow; Vasoconstriction; Vasoconstrictor Agents; Vasopressins | 2003 |
Antioxidant effect on renal scarring following infection of mannose-sensitive-piliated bacteria.
Renal scars have been considered to occur in later stages of chronic pyelonephritis. In our experimental pyelonephritis model, bacteria which possessed mannose-sensitive (MS) pili on the surface promoted renal scarring following inoculation to the renal parenchyma. Polyethylene glycol-modified superoxide dismutase (PEG-SOD) and 2-O-octadecylascorbic acid (CV3611) significantly suppressed scarring when administered orally or parenterally during the early stage of kidney infection with MS-piliated bacteria. These findings suggest that the superoxide and other active oxygens play an important role in renal scarring following infection and that PEG-SOD and CV3611 may be agents capable of preventing renal scarring following bacterial pyelonephritis. Topics: Administration, Oral; Animals; Antioxidants; Ascorbic Acid; Cicatrix; Disease Models, Animal; Drug Resistance; Escherichia coli; Escherichia coli Infections; Female; Fimbriae, Bacterial; Kidney; Mannose; Polyethylene Glycols; Pyelonephritis; Rats; Serratia Infections; Serratia marcescens; Superoxide Dismutase | 1992 |
Prophylaxis of experimental bacterial infection in mice by 1'-methylascorbigen.
Topics: Adjuvants, Immunologic; Animals; Ascorbic Acid; Bacterial Infections; Escherichia coli Infections; Indoles; Mice; Staphylococcal Infections | 1991 |
Response of White Leghorn chicks fed ascorbic acid and challenged with Escherichia coli or with corticosterone.
This study was undertaken to assess the effects of dietary ascorbic acid on the growth and immunoresponsiveness of chickens when subjected to particular types of stress. White Leghorn chicks were fed diets containing no supplemental ascorbic acid, and ascorbic-acid diet (330 ppm) for 2 days or for 19 days before challenge. Then, half of the females were inoculated with Escherichia coli; half of the males were challenged with dietary corticosterone (30 ppm) for 12 days; and the remaining chicks were maintained as controls. These chicks, reared under "good" husbandry procedures, did not realize advantages in growth or feed efficiency due to the short- or long-term consumption of diets containing ascorbic acid. Incubation with E. coli resulted in considerably higher heterophil-to-lymphocyte ratios 24 h after inoculation, and E. coli-induced mortality was higher for pullets on short-term ascorbic acid than for those on long-term or no ascorbic acid. Dietary corticosterone caused differences in body weight and the relative weights of certain organs, regardless of dietary levels of ascorbic acid. The antibody response to red-blood-cell antigens from sheep was enhanced in unchallenged cockerels (no dietary corticosterone) fed ascorbic acid on a long-term basis, but dietary corticosterone overshadowed the advantageous effects of dietary ascorbic acid. These data showed that the effects of supplemental ascorbic acid on growth and on immunoresponsiveness were related to the quality of the husbandry, length of supplemental feeding, age of the chicks, endogenous-exogenous balance for ascorbic acid, and the relationship with corticosterone. Topics: Animals; Antibody Formation; Ascorbic Acid; Body Weight; Chickens; Corticosterone; Erythrocytes; Escherichia coli Infections; Female; Granulocytes; Immunity; Leukocyte Count; Lymphocytes; Male; Organ Size; Poultry Diseases; Sex Characteristics | 1989 |
Progress in the treatment of chronic urinary tract infection--malacoplakia: current concepts.
Topics: Adult; Anti-Bacterial Agents; Ascorbic Acid; Bethanechol Compounds; Chronic Disease; Escherichia coli Infections; Female; Humans; Malacoplakia; Urinary Tract Infections | 1983 |
Predictability of methenamine efficacy based on type of urinary pathogen and pH.
This study involved 27 geriatric patients with asymptomatic chronic bacteriuria; all had indwelling Foley catheters. The treatment regimens (daily oral dosage) were: methenamine mandelate (MM) granules, 4 gm; MM, 4 gm, plus ascorbic acid, 4 gm; and MM, 4 gm, plus ascorbic acid, 4 gm, plus cranberry cocktail, 1 liter--administered according to a cross-over design. Proteus vulgaris, Pseudomonas aeruginosa and E. coli were the most common urinary organisms. Proteus organisms were more often found in alkaline than in acidic urines, but the type of pathogen had no influence on urinary pH. Urinary formaldehyde concentration [HCHO] was lower in patients with Proteus infection (17.7 micrograms/ml) than in those with Pseudomonas (21.9 micrograms/ml) or E. coli infection (21.8 micrograms/ml). However, for Proteus infection, [HCHO] was higher in patients receiving MM plus ascorbic acid than in those receiving MM alone. Addition of cranberry cocktail to ascorbic acid did not enhance urinary pH, [HCHO] or methenamine efficacy. Our data suggest that in Foley catheter patients with chronic asymptomatic bacteriuria secondary to Proteus, Pseudomonas or E. coli infection, the type of urinary pathogen or the urinary pH cannot be used to predict the efficacy of methenamine therapy either with or without urinary acidifying agents. Topics: Aged; Ascorbic Acid; Bacteriuria; Escherichia coli Infections; Female; Formaldehyde; Humans; Hydrogen-Ion Concentration; Klebsiella Infections; Male; Methenamine; Proteus Infections; Proteus vulgaris; Providencia; Pseudomonas Infections | 1981 |
[Use of glycocycline--a tetracycline preparation--for intravenous administration in surgery of suppurative diseases].
Topics: Adult; Ascorbic Acid; Chronic Disease; Escherichia coli Infections; Female; Humans; Infections; Lung Diseases; Male; Middle Aged; Osteomyelitis; Peritonitis; Pleural Diseases; Pneumonia; Staphylococcal Infections; Streptococcal Infections; Suppuration; Surgical Wound Infection; Tetracycline; Thiamine | 1967 |
Systemic Escherichia coli infection as a physiological stress in chickens.
Topics: Adrenal Glands; Animals; Antibody Formation; Ascorbic Acid; Bursa of Fabricius; Escherichia coli Infections; Glucosidases; Hemagglutination Tests; Organ Size; Poultry; Poultry Diseases; Sepsis; Spleen; Stress, Physiological | 1967 |