ascorbic-acid and Endophthalmitis

Topics: Adult; Aged; Amino Acids; Animals; Aqueous Humor; Ascorbic Acid; Blood Glucose; Child; Cyclic AMP; Drug Interactions; Drug Synergism; Endophthalmitis; Eye; Eye Diseases; Glaucoma; Humans; Intraocular Pressure; Kinetics; Middle Aged; Moxisylyte; Pharmacogenetics; Prostaglandins; Rabbits; Toxicology

We report a patient with risk factors for both microbial keratitis and endophthalmitis, which were initially challenging to distinguish. Cultures of corneal scrapings yielded several organisms, including an uncultivable Gram-negative rod, eventually identified as

Topics: Administration, Ophthalmic; Adult; Anti-Inflammatory Agents; Antioxidants; Ascorbic Acid; Ceftazidime; Cocaine; Cocaine-Related Disorders; Coinfection; Dopamine Uptake Inhibitors; Doxycycline; Endophthalmitis; Female; Gram-Positive Bacterial Infections; Humans; Keratitis; Kingella; Linezolid; Moxifloxacin; Neisseriaceae Infections; Ophthalmic Solutions; Prednisone; Propionibacterium acnes; Streptococcal Infections; Streptococcus; Substance Abuse, Intravenous; Tobramycin; Vancomycin; Voriconazole

A HPLC method for determination of ascorbic acid and dehydroascorbic acid in plasma and aqueous and vitreous humors of rabbits is described. Values for total ascorbic acid concentration found in this study are in agreement with those of previous investigators. Endotoxin-induced ocular inflammation caused a decrease in the concentration of ascorbic acid in the aqueous humor and an increase in the vitreous humor. The additional lack of correlation between levels of ascorbic acid in normal aqueous and vitreous humors from the same uninflamed eye indicates that the aqueous humor is not the source of vitreal ascorbic acid. The copper concentration of the aqueous humor is increased during ocular inflammation, most likely due to the influx of ceruloplasmin from plasma when the blood-aqueous barrier is disrupted. Ceruloplasmin caused a decrease in the amount of ascorbic acid in the aqueous humor in vitro and the vitreous humor in vivo. The presence of ceruloplasmin in the aqueous humor during inflammation thus may contribute to the decreased concentration of ascorbic acid in this fluid.

Topics: Animals; Aqueous Humor; Ascorbic Acid; Ceruloplasmin; Copper; Dehydroascorbic Acid; Endophthalmitis; Endotoxins; Eye Proteins; Rabbits; Vitreous Body

Topics: Animals; Aotus trivirgatus; Aqueous Humor; Ascorbic Acid; Columbidae; Ducks; Endophthalmitis; Eye; Eye Proteins; Guinea Pigs; Intraocular Pressure; Rabbits; Species Specificity

The absorption of ultraviolet radiation in the cornea of rabbit, cat, guinea pig, and rat has been studied. It turned out that about 80% of incident radiation is absorbed at the 290 nm wavelength limit in rabbit and cat, whereas the absorption is considerably lower in guinea pig and rat. In a model experiment based on conditions in the rabbit cornea epithelium, it is shown that the ascorbic acid in these cells is roughly responsible for ultraviolet ray absorption of the same order as proteins and nucleic acids in the 250-290 nm spectral range. This implies that for radiation below 290 nm wavelength also the ascorbic acid should be regarded as a potential target substance which may mediate photophthalmic damage to the tissue. The low absorption of the ascorbic acid compared to the other two components above 290 nm, indicates that the ascorbic acid contributes less in photophthalmic development due to solar radiation.

Topics: Animals; Ascorbic Acid; Cats; Cornea; Endophthalmitis; Epithelium; Guinea Pigs; Nucleic Acids; Proteins; Rabbits; Rats; Ultraviolet Rays