ascorbic-acid and Endometritis

Infection-induced endometritis is associated with infertility. The outcome with oral antibiotics remains poor. This study therefore investigates the role of ascorbic acid in resolving endometritis.. Animals with established oestrus cycles were inoculated with Escherichia coli. Two days post-inoculation, the animals were administered ascorbic acid (10, 100 and 1000 mg/kg) and amoxicillin (500 mg/kg) for 5 days. Other groups included water only and E. coli inoculated with no treatment. Body temperatures, weights and vaginal cytology were examined. On the sixth day, after anaesthesia, blood samples were obtained for haematological analysis. Uterine organs were weighed, ex-vivo functionality analysed and histopathological analysis performed.. Ascorbic acid (AA) (100 and 1000 mg/kg) regularized the cycle of the endometritic animals comparable to amoxicillin. AA (1000 mg/kg) and amoxicillin, significantly decreased (P < .05) the endometritis-induced increase in uterine weights, restored the endometrial architecture and significantly (P < .05) normalized uterine contractions to control values. Improved haematological profiles were additionally observed on treatment with ascorbic acid (100 and 1000 mg/kg).. AA compared favourably with amoxicillin in endometritis management, suggesting that AA can be used in the management of infection-induced endometritis, normal cycling and normal uterine function.

Topics: Amoxicillin; Animals; Anti-Bacterial Agents; Ascorbic Acid; Disease Models, Animal; Endometritis; Endometrium; Escherichia coli; Escherichia coli Infections; Female; Hematologic Tests; Humans; Mice; Mice, Inbred Strains; Uterus

A vast majority of the world buffalo resource is concentrated in tropical and subtropical countries. Apart from heat stress and poor nutritional availability, endometritis is one of the most commonly encountered reproductive problems limiting fertility and consequently productive potential of the species. As demonstrated recently, endometritis impairs growth and follicular fluid composition of the largest follicle in buffalo. In the present study, the effect of endometritis on luteal development, function, nitric oxide (NO), and ascorbic acid was investigated. Reproductive tracts were collected from 90 cyclic buffaloes at an abattoir and grouped into endometritic (n = 36) or non-endometritic (n = 54) buffaloes based on physical examination of uterine mucus, white side test, and uterine cytology. Samples with pus-containing mucus, positive reaction on white side test, and/or >5 % neutrophils were considered to be positive for endometritis. Corpora lutea were enucleated, weighed, classified into stages I to IV, and assayed for progesterone (P(4)), NO, and ascorbic acid concentrations. Endometritic buffaloes had lesser (P < 0.0001) luteal weight and P(4), NO, and ascorbic acid concentrations than non-endometritic buffaloes. The findings indicated that endometritis impairs corpus luteum development and function in buffalo. Reduced luteal NO and ascorbic acid concentrations during endometritis are novel findings.

Topics: Animals; Ascorbic Acid; Buffaloes; Corpus Luteum; Endometritis; Female; Follicular Fluid; Nitric Oxide; Ovarian Follicle; Progesterone

Pharmaceutic studies were carried out with a total of 89 cows affected with chronic endometritis, following up the biopharmacokinetic and therapeutic effect of amopen foaming tablets that contained amoxicillin trihydrate at the rate of 30,000 and 45,000 IU. Andreev's catheter was used to introduce the tablets into the uterus. It was found that the use of 600,000 IU amoxicillin developed therapeutic concentrations within the uterus up to the 48 h-72nd hour. Amoxicillin persisted in the blood serum up to the sixth hour. It was not eliminated by milk. The therapeutic effect with such cows (in which the causative organisms were susceptible to amoxicillin) to which the amopen tablets were introduced into the uterus (14 t. x 630,000 IU) three to four times at 48-hour intervals was shown to be 76 per cent. The use of 5 tablets of 45,000 IU at the 24th hour after the last insemination of cows that failed to conceive, exhibiting no symptoms of inflammation, raised the conception rate by 24.1 per cent.

Topics: Administration, Topical; Amoxicillin; Animals; Ascorbic Acid; Cattle; Cattle Diseases; Chronic Disease; Drug Combinations; Drug Evaluation; Endometritis; Female; Kinetics; Niacinamide; Riboflavin; Tablets; Thiamine; Time Factors; Uterus

Tested were two methods for the treatment of cows affected with acute endometritis after giving birth. The experiments were carried out with a total of 92 cows of the Bulgarian Brown breed kept under equal condtions of feeding and management. Two tests and one control groups were formed. The first group of cows (48) were treated with a bilateral epipleural block after Mossin. As a result 66.7 per cent of the cows conceived up to the 80th day after calving and 43.7 per cent at the first insemination. The service period of the impregnated cows of this group was 73.3 +/- 4.94 days, on an average. The second group of cows (25) were treated muscularly with a combination of 1 per cent magnesium sulphuricum solution (40 cu. cm), vitamin C (10 cu. cum), norsulphasol (5 g), and chloramphenicol (2 g). The treatment was repeated at a three-day interval. The results of the treatment accounted for 68 per cent impregnated cows up to an 80-day service period, and 48 per cent--at first insemination. The service period of the cows of this group lasted 69.3 +/- 6.0 days, on an average. The control group cows were treated at random with penicillin and streptomycin, muscularly. The conception rate at first insemination was 36.7 per cent, and within the range of an 80-day service period--47.02 per cent of the treated cows. The average service period for this group lasted 91.80 +/- 9.28 days.

Topics: Acute Disease; Animals; Ascorbic Acid; Cattle; Cattle Diseases; Chloramphenicol; Drug Evaluation; Drug Therapy, Combination; Endometritis; Female; Magnesium Sulfate; Nerve Block; Pregnancy; Procaine; Puerperal Infection; Sulfathiazoles

Topics: Abortion, Induced; Abortion, Spontaneous; Ascorbic Acid; Endometritis; Female; Flavonoids; Humans; Pathology; Prednisone; Pregnancy