ascorbic-acid and Collagen-Diseases

Topics: Adenocarcinoma, Scirrhous; Adult; Amino Acids; Animals; Ascorbic Acid; Cattle; Child; Collagen; Collagen Diseases; Female; Humans; Hydroxyproline; Lung; Marfan Syndrome; Mice; Procollagen-Proline Dioxygenase; Skin Diseases; Stomach Neoplasms

Topics: Adult; Age Factors; Anemia; Animals; Ascorbic Acid; Ascorbic Acid Deficiency; Bone Diseases, Developmental; Capillaries; Child; Collagen Diseases; Connective Tissue; Creatinine; Dentin; Gingival Hemorrhage; Hemorrhage; Humans; Hydroxyproline; Infant; Scurvy; Skin Diseases; Species Specificity

Topics: Animals; Ascorbic Acid; Bacteria; Bone Diseases; Chemical Phenomena; Chemistry; Collagen; Collagen Diseases; Endocrine System Diseases; Glycosides; Humans; Hydroxylation; Hydroxylysine; Hydroxyproline; Isomerism; Peptide Hydrolases; Plants; Polyribosomes; Procollagen-Proline Dioxygenase; Proline; Protein Precursors

Topics: Aldehydes; Animals; Ascorbic Acid; Collagen; Collagen Diseases; Connective Tissue; Depression, Chemical; Glycosaminoglycans; Guinea Pigs; Humans; Microbial Collagenase; Penicillamine; Rabbits; Rats; RNA, Messenger; RNA, Ribosomal; Schiff Bases; Transcription, Genetic

Topics: Adrenal Cortex Hormones; Adrenocorticotropic Hormone; Ascorbic Acid; Brain Diseases; Collagen Diseases; Cortisone; Glucocorticoids; Humans; Peptic Ulcer; Rheumatic Fever; Water-Electrolyte Balance

A double-blind controlled trial initiated in 1944 has led to the common narrative that a 10-mg daily vitamin C intake is adequate to prevent and treat impaired wound healing, and by inference, other collagen-related diseases such as heart disease or stroke. The WHO relies on this narrative to set the recommended nutrient intake for vitamin C. This narrative, however, is based on what is known as the eyeball method of data assessment. The 1944 trial published individual participant data on scar strength providing an opportunity to statistically probe the validity of the 10-mg narrative, something which has not yet been done. The findings show that a vitamin C intake that averages to 10 mg/d over a mean follow-up of 11.5 mo was associated with a 42% weakened scar strength when compared with 80 mg vitamin C intake/d (P < 0.001). The observed dose-response curve between scar strength and vitamin C intake suggests that the daily vitamin C intake needed to prevent collagen-related pathologies is in the range recommended by the National Academy of Medicine and the European Food Safety Authority (75 to 110 mg/d), not the WHO recommendation (45 mg/d). The findings also show that a vitamin C intake that averages to 65 mg/d over a mean follow-up of 6.5 mo failed to restore the normal wound-healing capacity of vitamin C-depleted tissues; such tissues had a 49% weaker scar strength when compared with nondepleted tissues (P < 0.05). Thus, average daily vitamin C intakes ∼50% higher than the WHO recommends may fail to treat existing collagen-related pathologies. It is concluded that the prior lack of statistical analyses of a landmark trial may have led to a misleading narrative on the vitamin C needs for the prevention and treatment of collagen-related pathologies.

Topics: Ascorbic Acid; Cicatrix; Collagen Diseases; Data Interpretation, Statistical; Double-Blind Method; History, 20th Century; Humans; Nutritional Requirements; Randomized Controlled Trials as Topic; Vitamins

Lathyrism is characterized by defective collagen synthesis due to inhibition of lysyl oxidase, an enzyme essential for interfibrillar cross-linking. The lathyritic agent beta-aminoproprionitrile (beta-APN) is considered an appropriate agent for studying connective tissue metabolism. We investigated the effects of ascorbic acid on collagen structure and serum cytokine levels in experimentally induced lathyrism. Forty Wistar rats weighing 200-300 g were used in the study: three test groups of 10 rats each (groups 2, 3 and 4) and 10 rats used as a control group (group 1). Experimental lathyrism was induced with daily subcutaneous injections of beta-APN in the test groups for 40 days. On the 40th day, skin biopsies were taken from the control group (group 1) and group 2, to evaluate the effect of beta-APN on dermal collagen. After the 40th day, 10 rats received ascorbic acid 100 mg/kg intraperitoneally daily for 15 days (group 3) and 10 rats (group 4) received no medication and served as a control for group 3. On the 55th day, skin biopsies were taken from groups 3 and 4. Serum concentrations of interleukin-6 and tumour necrosis factor-alpha were assessed in each group by enzyme-linked immunosorbent assay. Ultrastructural examination of the skin biopsies in group 1 revealed normal-appearing epidermal and dermal structures. Group 2 showed disorganization of the epidermis and collagen structure, and vacuolization of the endoplasmic reticulum in fibroblasts. In group 3, ultrastructural examination revealed significant improvement in the structure of dermal collagen after administration of ascorbic acid, whereas the changes in group 4 were unremarkable. Ascorbic acid administration significantly decreased the concentrations of serum cytokines in group 3 compared with group 2 (P < 0.001). Ascorbic acid administration significantly improved dermal collagen structure and serum cytokine levels in experimental lathyrism.

Topics: Animals; Ascorbic Acid; Collagen; Collagen Diseases; Interleukin-6; Lathyrism; Rats; Rats, Wistar; Tumor Necrosis Factor-alpha

Topics: Adolescent; Adult; Ascorbic Acid; Cardiovascular Diseases; Collagen Diseases; Electron Spin Resonance Spectroscopy; Flavonoids; Freeze Drying; Humans; Hypersensitivity; Middle Aged; Pneumonia

Topics: Animals; Ascorbic Acid; Bone Diseases; Carbon Isotopes; Cartilage; Chemical Phenomena; Chemistry; Chick Embryo; Collagen; Collagen Diseases; Connective Tissue; Connective Tissue Cells; Galactose; Glycine; Hydroxyproline; In Vitro Techniques; Ketoglutaric Acids; Proline; RNA, Messenger; RNA, Transfer

Topics: Ascorbic Acid; Collagen Diseases; Corrinoids; Cystitis; Cystitis, Interstitial; Drug Therapy; Humans; Lupus Erythematosus, Systemic; Pyridoxine; Scleroderma, Systemic; Ulcer; Vitamin B 12

Topics: Ascorbic Acid; Collagen Diseases; DNA; Eukaryota; Granulation Tissue; Granuloma; Guinea Pigs; Inflammation; Rats; Research; Silicosis; Toxicology

Topics: Antifibrinolytic Agents; Ascorbic Acid; Aspirin; Collagen; Collagen Diseases; Foot Diseases; Hemostatics; Humans; Vitamin K; Vitamins

Topics: Ascorbic Acid; Collagen; Collagen Diseases; Connective Tissue; Humans; Rheumatic Diseases; Scurvy; Vitamins