ascorbic-acid and Anemia--Aplastic

Forty-three cases of aplastic anemia were treated with fetal blood transfusion, Chinese medicinal herbs and Vit. C. The results showed that the effective rate of the treated group was 79.1%, among them, the chronic aplastic anemia (CAA) was 88.9%, acute aplastic anemia (AAA) was 62.5%, both rates were higher than that of the control group (with western medicine alone, n = 46). The difference of treatment results between two groups was highly significant. The mechanism was that the therapy could rebuild hematopoietic function, modulate immune function and improve microcosmic environment in bone marrow so that it promoted in all aspect the recovery of hematopoietic function in organism.

Topics: Adolescent; Anemia, Aplastic; Ascorbic Acid; Blood Transfusion; Child; Child, Preschool; Combined Modality Therapy; Drugs, Chinese Herbal; Female; Fetal Blood; Humans; Male

Total body irradiation (TBI) is a routine preconditioning procedure for the treatment of leukemia and aplastic anemia, prior to bone marrow transplantation (BMT). Ionizing radiation generates reactive oxygen derived species (ROS) that can be removed by antioxidants. Our purpose is to determine the antioxidant status of patients undergoing TBI by evaluating the oxidant stress and their antioxidant capacity.. We evaluated by cyclic voltammetry (CV) the total antioxidant capacity (TAC) in plasma of 14 patients undergoing TBI prior to BMT. The levels of the antioxidants, ascorbic acid (AA) and uric acid (UA) were determined by HPLC-ECD. The oxidant stress level was calculated by the ratio [dehydro ascorbic acid]/total ascorbic acid].. TAC was reduced by 36% (p < 0.02) but after 4 months recovered to a level 22% higher than before the treatment (p < 0.05). Both, AA and UA, decreased following irradiation by 84% (p < 0.02) and 24% (p < 0.05) respectively, but returned to a level of 21% and 320% after 4 months compared to baseline values. The changes in [UA] were affected by Allopurinol (xanthine oxidase inhibitor), given as a routine pretransplant therapy until day -1. The [dehydroascorcbic acid]/[total ascorbic acid] (%) was 45% (range of normal controls = 13.2 +/- 1.5%) and increased by 69% following TBI. In order to obtain a decrease in the TAC of plasma in vitro, comparable to that in vivo, a 1000 fold higher dose of irradiation was required.. TBI caused a pronounced decrease in antioxidant capacity and an excessive increase in oxidant stress. We assume that TBI alters antioxidant homeostasis greatly enhancing the stress damage. CV measurements may lead to a better understanding of the balance between oxidant stress and antioxidant utilization, and to a reconsideration of the routine use of Allopurinol as pretreatment for TBI, and antioxidant support before and/or after TBI.

Topics: Adolescent; Adult; Anemia, Aplastic; Antioxidants; Ascorbic Acid; Bone Marrow Transplantation; Child; Chromatography, High Pressure Liquid; Dehydroascorbic Acid; Female; Humans; Leukemia, Myeloid, Acute; Male; Middle Aged; Oxidative Stress; Uric Acid; Whole-Body Irradiation

Peripheral blood lymphocytes from eight Fanconi anemia (FA) patients, 14 FA heterozygotes, and nine normal subjects have been tested for their susceptibility to chromosomal breakage induction by diepoxybutane (DEB) and by two peroxides. In addition, the effect of five antioxidants was investigated in standard cultures and in cultures stressed either with DEB or with butylhydroperoxide (BHP) or with hydrogen peroxide (H2O2). DEB, BHP, and H2O2 dramatically increased the chromosomal breakage levels in homozygous and heterozygous FA cells. A partial correction of chromosomal instability was obtained by treating the patients' lymphocytes with antioxidants. A "protective" effect was also noted in the DEB or peroxide-stressed lymphocytes of patients and heterozygotes, grown in the presence of antioxidants.

Topics: Anemia, Aplastic; Antioxidants; Ascorbic Acid; Cells, Cultured; Chromosome Aberrations; Cross-Linking Reagents; Cysteine; Epoxy Compounds; Ethers, Cyclic; Fanconi Anemia; Glutathione; Heterozygote; Homozygote; Humans; Hydrogen Peroxide; Lymphocytes; Mercaptoethanol; Peroxides; Tiopronin

Topics: Anemia, Aplastic; Antioxidants; Ascorbic Acid; Chromosome Aberrations; Cysteine; Epoxy Compounds; Ethers, Cyclic; Fanconi Anemia; Humans; Hydrogen Peroxide; Mercaptoethanol; Mutagens; Oxygen Consumption; Peroxides; tert-Butylhydroperoxide

Chronic refractory anemia associated with congenital hypoplastic anemia (CHA, Blackfan-Diamond syndrome) and with the 5q-syndrome may require chronic transfusion therapy to sustain life. Hemosiderosis and death from chronic iron overload may result from such a program. The effect of subcutaneous (SC) deferoxamine (DF) and supplemental oral vitamin C (vit. C) on urinary iron excretion was studied in two patients with congenital hypoplastic anemia and one patient with 5q-syndrome. In the two patients with CHA, urinary iron excretion in response to DF given SC over 24 hours was comparable to the results following intravenous (I.V.) administration. Both of these cases had low levels of plasma ascorbate on initial evaluation and excreted more iron in response to two different doses of DF after they had received supplemental vit C and their stores were repleted. Significant iron excretion occurred in all three patients for 12 hours during the SC infusion of DF and for 12 hours after the end of the infusion. In all three patients, increasing the dose of DF up to 3-4 g given SC over 12 hours resulted in a linear increase in iron excretion. Once normal body stores of ascorbate were achieved by oral supplementation, increasing doses of vit C did not appear to cause a further increment in iron excretion. DF administered by a slow SC infusion appears to be an effective approach to iron overload in patients with refractory anemia and hemosiderosis secondary to chronic transfusions. Only small amounts of supplemental vit. C necessary to sustain adequate body stores are required for optimal iron excretion.

Topics: Administration, Oral; Anemia, Aplastic; Ascorbic Acid; Blood Transfusion; Child; Chromosome Aberrations; Chromosome Disorders; Chromosomes, Human, 4-5; Deferoxamine; Female; Hemosiderosis; Humans; Injections, Subcutaneous; Iron; Male; Middle Aged; Syndrome

Absorption of 59Fe ferrous sulphate was measured in patients with a variety of iron-loading anaemias both before and during subcutaneous infusions of desferrioxamine. The infusions were associated with a reduction in absorption (P less than 0-025). This effect was not altered by saturation with ascorbic acid.

Topics: Administration, Oral; Adolescent; Adult; Anemia, Aplastic; Anemia, Sideroblastic; Ascorbic Acid; Binding Sites; Blood Transfusion; Deferoxamine; Drug Synergism; Erythrocytes; Female; Ferritins; Ferrous Compounds; Humans; Infusions, Parenteral; Intestinal Absorption; Iron; Iron Radioisotopes; Isotope Labeling; Male; Thalassemia

Topics: Adrenal Cortex Hormones; Androstanes; Anemia, Aplastic; Anemia, Hemolytic; Anemia, Hypochromic; Anemia, Macrocytic; Anemia, Pernicious; Ascorbic Acid; Blood Transfusion; Humans; Iron; Iron-Dextran Complex; Ketones; Splenectomy; Vitamin B 12

Topics: Anemia, Aplastic; Animal Feed; Animals; Ascorbic Acid; Blood Volume Determination; Chickens; Copper; Hemorrhage; Iron; Poultry Diseases; Quinoxalines; Vitamin B 12

Topics: Adolescent; Adult; Anemia, Aplastic; Angiography; Ascorbic Acid; Blood Transfusion; Blood Vessel Prosthesis; Child; Folic Acid; Humans; Infant; Infrared Rays; Lectins; Male; Phenindione; Photography; Testosterone; Triamcinolone

Topics: Anemia; Anemia, Aplastic; Ascorbic Acid; Bone Marrow; Bone Marrow Transplantation; Humans; Pyridoxine; Testosterone; Twins; Vitamin B 12

Topics: Anemia; Anemia, Aplastic; Ascorbic Acid; Benzene; Corrinoids; Diagnosis; Humans; Occupational Diseases; Pancytopenia; Pyridoxine; Toxicology; Vitamin B 12

Topics: Adrenal Cortex Hormones; Adrenocorticotropic Hormone; Anemia; Anemia, Aplastic; Ascorbic Acid; Classification; Corrinoids; Electrocardiography; Folic Acid; Humans; Liver Extracts; Vitamin B 12

Topics: Anemia; Anemia, Aplastic; Ascorbic Acid; Bilirubin; Blood Cell Count; Blood Chemical Analysis; Blood Transfusion; Bone Marrow Examination; Child; Flavonoids; Folic Acid; Hematopoietic System; Humans; Infant; Infant, Newborn; Leukopenia; Osteoporosis; Prednisolone; Prednisone; Pyridoxine; Radiography; Riboflavin; Testosterone; Thiamine; Thrombocytopenia; Vitamin B 12

Topics: Anemia; Anemia, Aplastic; Ascorbic Acid; Blood Transfusion; Child; Corrinoids; Cortisone; Diagnosis; Humans; Iron; Rutin; Vitamin B 12; Vitamin K