ascorbic-acid and Amyloidosis

Topics: Amyloidosis; Ascorbic Acid; Biopsy; Humans; Hypoproteinemia; Kidney; Kidney Diseases; Liver Extracts; Microscopy, Electron; Polyuria; Proteinuria

G555F mutant of Fibrinogen A alpha-chain (FGA) is reported to be associated with kidney amyloidosis. In the current study, we have modelled the G555F mutant and examined the mutation's effect on the structural and functional level. We have also docked Vitamin C and D3 on the mutant's amyloidogenic region to identify if these vitamins can bind amyloidogenic regions. Further, we analyzed if they could prevent or modulate amyloid formation by stopping critical interactions in amyloidogenic regions in FGA. We used the wild type FGA model protein as a control. Our docking and molecular dynamics simulation results indicate stronger Vitamin D3 binding than Vitamin C to the amyloidogenic region of the mutant protein. The RMSD, radius of gyration, and RMSF values were higher for the G555F mutant than the FGA wild type protein. Overall, the results support these vitamins' potential as a therapeutic and anti-amyloidogenic agent for FGA renal amyloidosis.

Topics: Amyloidosis; Ascorbic Acid; Cholecalciferol; Fibrinogen; Humans; Vitamins

Amyloidosis results from protein infiltration of the extracellular space of organs and tissues. Several amyloidosis proteins have been identified. Protein AL, (deriving from immunoglobulin light chain), protein AA and prealbumin are the most involved in this disease. When AL amyloidosis involves the heart, the illness is often terminal. Most clinical symptoms are heart failure and arrhythmia or block conduction. This case was characterised by the unusual combination of hypertension and amyloidosis. The diagnosis suggested by the echocardiographic but was confirmed by the damaged organ's biopsy. The present case concerns a young woman, who has hypertension and a pulmonary oedema. The echocardiographic scan showed a septal hypertrophy with a shining and granite-like aspect which is compatible with heart amyloidosis. Systolic and diastolic disorder with mitral and aortic regurgitation were also revealed. The kidney and rectum biopsies confirmed amyloidosis AL of the Kappa dysglobulinemia type, without extraosseous plasmocytoma. The heart and kidney failure symptoms disappeared after treatment with diuretics and ACE inhibitors.

Topics: Adult; Amyloidosis; Angiotensin-Converting Enzyme Inhibitors; Ascorbic Acid; Biopsy; Cardiomegaly; Colchicine; Diuretics; Echocardiography; Female; Heart Failure; Heart Valve Diseases; Humans; Hypertension; Immunoglobulin kappa-Chains; Nephritis; Paraproteinemias; Pulmonary Edema; Radiography; Rectum

A patient with quiescent rheumatoid arthritis, amyloidosis and chronic renal failure developed an inflamed knee. Intracellular bipyramidal crystals characteristic of oxalate were found and are suggested as the cause of the acute arthritis. Since the patient had been treated with vitamin C, this precursor of oxalate is proposed as a possible factor in the crystal deposition.

Topics: Amyloidosis; Arthritis, Rheumatoid; Ascorbic Acid; Humans; Kidney Failure, Chronic; Male; Middle Aged; Oxalates; Oxalic Acid; Peritoneal Dialysis; Radiography; Synovial Fluid

Topics: Amyloidosis; Animals; Ascorbic Acid; Diet; Mice

Ascorbic acid (AA) effects on the development of lymphoproliferative process according to the type of a reticular variant of Hodgkin's disease have been investigated in SJL/J mice. An oral AA injection in a 0.05% aqueous solution for 6-8 months (0.625 mg/kg) with a total dose of 0.5 per mouse has not produced any side effects, has reduced both the rate of tumour process development (from 80.5% in control to 68.0% in experiment) and the degree of its extension, and also has led to a statistically significant decrease in the frequency of development of general amyloidosis.

Topics: Aging; Amyloidosis; Animals; Ascorbic Acid; Dose-Response Relationship, Drug; Drug Evaluation, Preclinical; Female; Hodgkin Disease; Lymphoproliferative Disorders; Male; Mice; Mice, Inbred Strains

Ascorbic acid was found to accelerate amyloid degradation in an experimental animal model. Based on experiments in vitro which demonstrated the ability of ascorbic acid to restore the amyloid-degrading activity of amyloidotic human serum, the effect of orally administered ascorbic acid was tested in casein-induced murine amyloidosis. Histopathological examination of splenic tissue of mice killed at different times after the termination of the amyloidogenic stimulus showed a markedly decreased amyloid deposition in ascorbic acid-treated animals as compared to the controls. The effect of ascorbic acid was to a certain degree dose-dependent. Colchicine blocked amyloid synthesis when administered during amyloid induction. In animals which were given the drug during the post-induction period it had no effect. The amyloid-degrading activity of mouse serum was reduced in amyloidotic mice. Administration of ascorbic acid partially restored the amyloid-degrading activity of these animals.

Topics: Amyloidosis; Animals; Ascorbic Acid; Colchicine; Dose-Response Relationship, Drug; Mice; Spleen; Splenic Diseases

It has been claimed that ascorbic acid enhances the in vitro degradation of AA amyloid fibrils. This raises the possibility that ascorbic acid may be of benefit in systemic AA amyloidosis, a condition with serious morbidity and mortality for which there is as yet no specific treatment. The effect was therefore tested of oral or injected supplements of ascorbic acid on the induction of AA amyloidosis in mice. Amyloid was induced either by repeated injections of casein or by injection of 'amyloid enhancing factor' and silver nitrate. Mice with established amyloidosis were also treated with additional ascorbic acid. Despite the fact that plasma ascorbic acid levels were significantly higher in orally supplemented mice than in controls there was no demonstrable effect on the induction, the extent and distribution or the progression of amyloidosis.

Topics: Amyloid; Amyloidosis; Animals; Ascorbic Acid; Caseins; Male; Mice; Mice, Inbred C57BL; Mice, Inbred CBA; Silver Nitrate; Tissue Extracts

Topics: Adolescent; Adult; Aged; Amyloidosis; Arthritis, Juvenile; Arthritis, Rheumatoid; Ascorbic Acid; Child; Child, Preschool; Dimethyl Sulfoxide; Drug Evaluation; Glomerular Filtration Rate; Humans; Kidney; Middle Aged; Proteinuria; Scleroderma, Systemic; Sjogren's Syndrome; Ultrasonics

Topics: Age Factors; Aging; Amyloid; Amyloidosis; Antioxidants; Ascorbic Acid; Ascorbic Acid Deficiency; Free Radicals; Humans; Molecular Conformation; Protein Denaturation; Proteins; Time Factors; Vitamin E

Topics: Adult; Aged; Amyloidosis; Ascorbic Acid; Biopsy; Female; Humans; Kidney Diseases; Male; Middle Aged; Tuberculosis

Topics: Adult; Aged; Alkaline Phosphatase; Amyloidosis; Ascorbic Acid; Carcinoma; Glycosaminoglycans; Histocytochemistry; Humans; Lymphatic Metastasis; Male; Methods; Microscopy, Electron; Middle Aged; Nucleic Acids; Thyroid Gland; Thyroid Neoplasms

Topics: Amyloidosis; Ascorbic Acid; Conjunctiva; Conjunctival Diseases; Conjunctivitis; Deficiency Diseases; Diagnosis; Eye Diseases; Humans; Immunoglobulin Light-chain Amyloidosis; Liver Extracts; Surgical Procedures, Operative; Triamcinolone; Vitamin A

Topics: Alpha-Globulins; Amyloidosis; Animals; Ascorbic Acid; Beta-Globulins; Blood Protein Electrophoresis; Blood Proteins; gamma-Globulins; Glycoproteins; Guinea Pigs; Histocytochemistry; Liver; Research; Scurvy; Spleen; Toxicology; Turpentine

Topics: Amyloid; Amyloidosis; Ascorbic Acid; Asthma; Cortisone; Nephrosis; Uremia; Vitamins

Topics: Amyloidosis; Ascorbic Acid; Cortisone; Humans; Vitamins

Topics: Amyloidosis; Animals; Ascorbic Acid; Ascorbic Acid Deficiency; Guinea Pigs; Vitamins