ascorbic-acid and Airway-Obstruction

Topics: Airway Obstruction; Animals; Ascorbic Acid; Constriction; Guinea Pigs; Histamine; Humans; Placebos; Propranolol; Respiratory Function Tests; Trachea

To measure the ascorbic acid (AA) concentration in bronchoalveolar lavage fluid (BALF) and cellular glutathione peroxidase (cGPx) activity in RBCs and WBCs from peripherally obtained blood and in cells from BALF to determine whether differences existed between the 2 major redox systems in recurrent airway obstruction (RAO)-affected and -nonaffected (control) horses and between systemic and local pulmonary responses in the glutathione redox system.. 16 adult horses in pairs: 8 healthy (control) and 8 RAO-affected horses.. Physical examination data and biological samples were collected from horses before (remission), during, and after (recovery) environmental challenge with dusty straw and hay. At each stage, BALF cell AA concentration and RBC, WBC, and BALF cell cGPx activity were measured.. Compared with control horses, RAO-affected horses had significantly higher cGPx activity in RBCs at all points and in WBCs during remission and challenge. The BALF cell cGPx activity was higher in RAO-affected horses during recovery than during remission The BALF cell AA concentration did not differ significantly in control horses at any point, but total and free AA concentrations were significantly lower in RAO-affected horses during the challenge period than during remission and recovery periods.. High cGPx activity suggested this redox system was upregulated during exposure to dusty straw and hay to combat oxidative stress, as AA was depleted in RAO-affected horses. The relative delay and lack of comparative increase in cGPx activity within the local environment (represented by BALF cells), compared with that in RBCs and WBCs, might contribute to disease in RAO-affected horses.

Topics: Airway Obstruction; Animals; Ascorbic Acid; Bronchoalveolar Lavage Fluid; Erythrocytes; Glutathione Peroxidase; Horse Diseases; Horses; Recurrence; Reference Values

Topics: Airway Obstruction; Anesthesia, General; Anesthetics, Inhalation; Anesthetics, Intravenous; Ascorbic Acid; Child, Preschool; Edema; Fentanyl; Gingivitis; Hemorrhage; Humans; Intubation, Intratracheal; Laryngeal Masks; Leg; Male; Methyl Ethers; Propofol; Scurvy; Sevoflurane; Vitamins

The relationship between hydrogen peroxide (H2O2) concentration in expired breath condensate (EBC) and cytology of the respiratory tract obtained from tracheal wash (TW) or bronchoalveolar lavage (BAL), and epithelial lining fluid (ELF) antioxidant status is unknown. To examine this we analysed the concentration of H2O2 in breath condensate from healthy horses and horses affected by recurrent airway obstruction (RAO), a condition considered to be an animal model of human asthma. The degree of airway inflammation was determined by assessing TW inflammation as mucus, cell density and neutrophil scores, and by BAL cytology. ELF antioxidant status was determined by measurement of ascorbic acid, dehydroascorbate, reduced and oxidised glutathione, uric acid and alpha-tocopherol concentrations. RAO-affected horses with marked airway inflammation had significantly higher concentrations of breath condensate H2O2 than control horses and RAO-affected horses in the absence of inflammation (2.0 +/- 0.5 micromol/l. 0.4 +/- 0.2 micromol/l and 0.9 +/- 0.2 micromol/l H2O2, respectively; p < 0.0001). The concentration of breath condensate H2O2 was related inversely to the concentration of ascorbic acid in ELF (r = -0.80; p < 0.0001) and correlated positively with TW inflammation score (r = 0.76, p < 0.0001) and BAL neutrophil count (r = 0.80, p < 0.0001). We conclude that the concentration of H2O2 in breath condensate influences the ELF ascorbic acid concentration and provides a non-invasive diagnostic indicator of the severity of neutrophilic airway inflammation.

Topics: Airway Obstruction; Animals; Ascorbic Acid; Breath Tests; Bronchoalveolar Lavage Fluid; Bronchoscopy; Cell Count; Dehydroascorbic Acid; Diagnosis, Differential; Disease Models, Animal; Glutathione; Horses; Hydrogen Peroxide; Inflammation; Mucus; Neutrophils; Respiratory Mucosa; Respiratory System; Spectrophotometry

Topics: Airway Obstruction; Antioxidants; Ascorbic Acid; Bronchoalveolar Lavage Fluid; Glutathione; Humans; Lung Neoplasms; Nitrites; Oxidative Stress; Proteins; Regression Analysis; Sputum; Uric Acid

Topics: Airway Obstruction; Animals; Antioxidants; Ascorbic Acid; Bronchoalveolar Lavage Fluid; Female; Horse Diseases; Horses; Lipid Peroxidation; Male; Tocopherols

Dietary antioxidants, waist circumference, and pulmonary function were measured in the Fourth Scottish MONICA cross-sectional survey of 865 men and 971 women aged 25-64 years. Waist circumference was inversely related to forced expiratory volume in the first second (FEV1) and forced vital capacity (FVC), even after adjustment for age, height, weight, working status, energy intake, and smoking variables in a multiple linear regression model (men: beta = -0.017 for FEV1 l/cm, p < 0.01 and beta = -0.008 for FVC, p = 0.04; women: beta = -0.009 for FEV1, p < 0.01 and beta = -0.007 for FVC, p = 0.01). After additional adjustment for waist circumference, estimated vitamin C and beta-carotene intakes were positively associated with lung function in men (vitamin C: beta = 0.102 for FEV1 l/mg/day, p = 0.03; beta-carotene: beta = 0.073 for FVC l/g/day, p = 0.02). Retinol and vitamin E were not significantly related to lung function for either sex. A case-control study of airway obstruction showed that waist circumference was significantly associated, while vitamin C could be protective. The study suggests that adequate intake of antioxidants and avoidance of increasing girth could help to preserve lung function.

Topics: Adult; Airway Obstruction; Anthropometry; Antioxidants; Ascorbic Acid; beta Carotene; Body Constitution; Case-Control Studies; Cross-Sectional Studies; Diet; Female; Forced Expiratory Volume; Humans; Linear Models; Male; Middle Aged; Obesity; Scotland; Vital Capacity; Vitamin A; Vitamin E

Topics: Airway Obstruction; Ascorbic Acid; Asthma; Humans; Vitamins