arginine-aspartate and Neoplasm-Metastasis

To test the antimetastatic effects of Arg-Asp (RD) on salivary adenoid cystic carcinoma (SACC-LM) in vivo.. RD was administered orally to experimental metastasized nude mice. The pulmonary metastatic foci number and survival were determined to assay the antimetastatic effects of RD.. 30 mg/kg, 120 mg/kg of RD demonstrated an inhibitory effect on the pulmonary metastatic foci formation. All of the tested dosages (7.5 mg/kg, 30 mg/kg, 120 mg/kg) of RD prolonged the survival.. Oral administration of RD has a antimetastatic effect on SACC-LM. RD is low toxicity.

Topics: Animals; Antineoplastic Agents; Arginine; Aspartic Acid; Carcinoma, Adenoid Cystic; Dose-Response Relationship, Drug; Female; Humans; Lung Neoplasms; Mice; Mice, Inbred BALB C; Neoplasm Metastasis; Salivary Gland Neoplasms

To test the effects of arginine-aspartate (RD) on metastasis of salivary cystic carcinoma (SACC).. Highly metastatic SACC cell line SACC-LM was used in the study. The effect of RD on tumor cell-ECM adhesion was assayed by MTT technique. The effect of RD on invasion and migration of SACC-LM were evaluated using modified boyden chamber.. RD inhibited tumor cell fibronectin adhesion at 5 mg/L concentration, but no effects on tumor cell laminin and tumor cell type IV collagen adhesion. RD inhibited the invasion at 1, 5, 25 mg/L, and inhibited the migration only at 25 mg/L.. The result shows that the RD has an antimetastatic effect on SACC-LM cells in vitro.

Topics: Antineoplastic Agents; Arginine; Aspartic Acid; Carcinoma, Adenoid Cystic; Cell Adhesion; Extracellular Matrix; Humans; Neoplasm Metastasis; Salivary Gland Neoplasms