apyrase has been researched along with Tuberculosis--Pulmonary* in 3 studies
3 other study(ies) available for apyrase and Tuberculosis--Pulmonary
| Article | Year |
|---|---|
The role of T regulatory cell-associated markers in monitoring tuberculosis treatment completion and failure.
Monitoring tuberculosis (TB) treatment success is crucial for clinical decision-making. The only available tool in this regard is sputum microscopy, but it has demerits. Moreover, in case of smear negatives and extrapulmonary TB, an efficient tool is still sought for. Therefore, we evaluated T regulatory cell (Treg)-associated markers (CD25, CD39, and FoxP3) and cellular subsets in monitoring treatment success in treatment-completed groups. Expression profile of various markers and subsets were compared real time among treatment-naive pulmonary TB patients (TN-PTB), followed-up treatment-completed (TC-fu) cohort, and a not followed-up (TC-nfu) cohort. Peripheral blood mononuclear cells from various groups were incubated overnight and were stained with antibodies for specific markers and studied by flow cytometry. In both the treatment-completed groups, a decline in frequencies of CD25 Topics: Adolescent; Adult; Antigens, CD; Antitubercular Agents; Apyrase; Biomarkers; Cell Separation; Cohort Studies; Female; Flow Cytometry; Forkhead Transcription Factors; Humans; Interleukin-2 Receptor alpha Subunit; Male; Middle Aged; Mycobacterium tuberculosis; T-Lymphocytes, Regulatory; Treatment Failure; Treatment Outcome; Tuberculosis, Pulmonary; Young Adult | 2018 |
Regulatory T-cell subsets in response to specific Mycobacterium tuberculosis antigens in vitro distinguish among individuals with different QTF and TST reactivity.
Regulatory T cells (Tregs), a subset of CD4+ T cells related with immune regulation, have been associated with active and latent tuberculosis infection (LTBI). Treg frequencies were evaluated by multicolor flow cytometry (FC) in peripheral blood mononuclear cells (PBMCs) stimulated with mycobacterial antigens ESAT-6, CFP-10, and TB7.7 to assess their capacity to distinguish subjects with different reactivity to the QuantiFERON-TB® Gold In-Tube (QFT-IT) test and the tuberculin skin test (TST). Increased frequencies of CD4+CD25highCD39+ cells were found for the [TST+, QTF+] compared with the [TST+, QTF-] group. Also, higher frequencies were observed for the [TST+, QTF+] compared with the [TST+, QTF-] and [TST-, QTF-] groups in CD4+CD25highFoxp3+ and CD4+CD25highCD39+Foxp3+ populations. Receiver operating characteristics (ROC curve) analysis confirmed these discriminating results. QFT-IT and TST quantitative values correlated with several Treg population frequencies. Topics: Adult; Antigens, Bacterial; Antigens, CD; Apyrase; CD4-Positive T-Lymphocytes; Cohort Studies; Female; Forkhead Transcription Factors; Humans; In Vitro Techniques; Interferon-gamma Release Tests; Interleukin-2 Receptor alpha Subunit; Interleukin-7 Receptor alpha Subunit; Latent Tuberculosis; Leukocytes, Mononuclear; Male; Middle Aged; Mycobacterium tuberculosis; T-Lymphocyte Subsets; T-Lymphocytes, Regulatory; Tuberculin Test; Tuberculosis, Pulmonary; Young Adult | 2015 |
Characterization of regulatory T cells identified as CD4(+)CD25(high)CD39(+) in patients with active tuberculosis.
Forkhead box P3 (FoxP3) is a transcription factor whose expression characterizes regulatory T cells (T(reg)), but it is also present on activated T cells, thus hindering correct T(reg) identification. Using classical markers for T(reg) recognition, discordant results were found in terms of T(reg) expansion during active tuberculosis (TB) disease. Recently CD39 has been shown to be an accurate marker for T(reg) detection. The objectives of this study were: (i) to identify T(reg) expressing CD39 in patients with TB and to compare the results with those obtained by the standard phenotypic markers; (ii) to evaluate if T(reg) are expanded in vitro by exogenous interleukin (IL)-2 or by antigen-specific stimulation; and (iii) to characterize T(reg) function on the modulation of antigen-specific responses. We enrolled 13 patients with pulmonary TB and 12 healthy controls. T(reg) were evaluated by flow cytometry ex vivo and after antigen-specific in vitro stimulation using CD25, FoxP3, CD127 and CD39 markers. Results indicate that CD39(+) cells within the CD4(+)CD25(high) cells have T(reg) properties (absence of interferon-gamma production and transforming growth factor-beta1 release upon stimulation). Ex vivo analysis did not show significant differences between TB patients and controls of T(reg) by classical or novel markers. In contrast, a significantly higher percentage of T(reg) was found in TB patients after antigen-specific stimulation both in the presence or absence of IL-2. Depletion of CD39(+) T(reg) increased RD1-specific responses significantly. In conclusion, CD39 is an appropriate marker for T(reg) identification in TB. These results can be useful for future studies to monitor Mycobacterium tuberculosis-specific response during TB. Topics: Adult; Aged; Antigens, CD; Apyrase; Bacterial Proteins; Biomarkers; Cells, Cultured; Cytokines; Female; Humans; Immunophenotyping; Interleukin-2; Interleukin-2 Receptor alpha Subunit; Male; Middle Aged; T-Lymphocyte Subsets; T-Lymphocytes, Regulatory; Tuberculosis, Pulmonary; Young Adult | 2009 |