apyrase has been researched along with Latent-Tuberculosis* in 2 studies
2 other study(ies) available for apyrase and Latent-Tuberculosis
| Article | Year |
|---|---|
Regulatory T-cell subsets in response to specific Mycobacterium tuberculosis antigens in vitro distinguish among individuals with different QTF and TST reactivity.
Regulatory T cells (Tregs), a subset of CD4+ T cells related with immune regulation, have been associated with active and latent tuberculosis infection (LTBI). Treg frequencies were evaluated by multicolor flow cytometry (FC) in peripheral blood mononuclear cells (PBMCs) stimulated with mycobacterial antigens ESAT-6, CFP-10, and TB7.7 to assess their capacity to distinguish subjects with different reactivity to the QuantiFERON-TB® Gold In-Tube (QFT-IT) test and the tuberculin skin test (TST). Increased frequencies of CD4+CD25highCD39+ cells were found for the [TST+, QTF+] compared with the [TST+, QTF-] group. Also, higher frequencies were observed for the [TST+, QTF+] compared with the [TST+, QTF-] and [TST-, QTF-] groups in CD4+CD25highFoxp3+ and CD4+CD25highCD39+Foxp3+ populations. Receiver operating characteristics (ROC curve) analysis confirmed these discriminating results. QFT-IT and TST quantitative values correlated with several Treg population frequencies. Topics: Adult; Antigens, Bacterial; Antigens, CD; Apyrase; CD4-Positive T-Lymphocytes; Cohort Studies; Female; Forkhead Transcription Factors; Humans; In Vitro Techniques; Interferon-gamma Release Tests; Interleukin-2 Receptor alpha Subunit; Interleukin-7 Receptor alpha Subunit; Latent Tuberculosis; Leukocytes, Mononuclear; Male; Middle Aged; Mycobacterium tuberculosis; T-Lymphocyte Subsets; T-Lymphocytes, Regulatory; Tuberculin Test; Tuberculosis, Pulmonary; Young Adult | 2015 |
Circulating mycobacterial-reactive CD4+ T cells with an immunosuppressive phenotype are higher in active tuberculosis than latent tuberculosis infection.
Previous studies suggest that control of Mycobacterium tuberculosis infection is compromised by the activity of regulatory T cells, including those that express CD39, an ectonucleotidase with immunosuppressive properties. Here, we examine the role of CD39 on CD4+ T cells reacting to M. tuberculosis antigens.. Cryopreserved PBMC from patients with active TB (n = 31) or individuals with LTBI (n = 30) were cultured with PPD, ESAT-6 or CFP-10 and antigen-reactive CD4+ T cells assessed by: A) intracellular expression of interferon-gamma (IFN-γ), tumour necrosis factor alpha (TNF-α) and interleukin (IL)-2, B) co-expression of CD25 and CD134 with or without CD39, and C) production of IFN-γ, TNF-α and IL-10 in culture supernatants.. Active TB patients were not differentiated from individuals with LTBI by intracellular expression of IFN-γ, TNF-α or IL-2 (alone or together), nor by co-expression of CD25 and CD134. However, active TB patients exhibited higher proportions of CD25+, CD134+, CD4+ T cells expressing CD39 in response to all antigens (p ≤ 0.022). Furthermore, in response to PPD, CD39 expression on CD25+, CD134+, CD4+ T cells correlated with IL-10 production (r = 0.41, p = 0.005) and inhibition of CD39 decreased IL-10 production.. Antigen-reactive CD4+ T cells expressing CD39 are more abundant in active TB than LTBI and are associated with production of the immunosuppressive cytokine IL-10. Modulating the effects of CD39 might enhance cellular immune responses against M. tuberculosis. Topics: Adult; Antigens, Bacterial; Antigens, CD; Apyrase; Cells, Cultured; Coculture Techniques; Female; Humans; Immune Tolerance; Immunophenotyping; Interferon-gamma; Interleukin-10; Latent Tuberculosis; Lymphocyte Activation; Male; Mycobacterium tuberculosis; Phenotype; Signal Transduction; T-Lymphocytes, Regulatory; Tuberculosis; Tumor Necrosis Factor-alpha | 2014 |