apyrase and Hypersensitivity

Mast cells (MCs) play a critical role in allergic inflammation. Although purinergic signalling is implicated in the regulation of various immune responses, its role in MC function is not fully understood. In this study, we investigated the regulatory role of purinergic signalling in MC degranulation, using mouse bone marrow-derived mast cells (BMMCs). Notably, BMMCs expressed various functional P2 adenosine triphosphate (ATP) receptors, including ionotropic P2X4 and P2X7, involved in the regulation of BMMC degranulation. Thus, P2X7 receptor activation induced a marked degranulation from BMMCs directly. Although P2X4 receptor activation did not independently induce degranulation, it significantly potentiated the degranulation triggered by antigen-induced, high-affinity IgE receptor (FcεRI) stimulation. In addition, ATP synergistically augmented degranulation induced by adenosine A

Topics: 5'-Nucleotidase; Adenosine Triphosphate; Animals; Antigens, CD; Apyrase; Bone Marrow Cells; Cell Degranulation; Cells, Cultured; Extracellular Space; Humans; Hypersensitivity; Mast Cells; Mice; Mice, Inbred C57BL; Receptor, Adenosine A3; Receptors, IgG; Receptors, Purinergic P2X4; Receptors, Purinergic P2X7; Signal Transduction