aprotinin and Burns--Chemical

The study of the influence of hordox, in treatment of experimental alkaline burn of the cornea, on the activity of trypsin-like proteases, elastases, callicreine, beta-N-acetylglucosaminidase and beta-glucuronidase in a tear fluid has shown that activity of these enzymes in a tear after burn remarkably increases, especially within first 24 hours and at the end of the second week after burn. In treatment by hordox, the activity of all enzymes in the tear, except elastase, reduces as compared with untreated animals, that speaks about antiinflammatory action of the preparation. On the basis of the data obtained it is suggested that investigation of hydrolytic enzymes in a tear can serve as a criterion for aimed correction of proteolysis in inflammatory processes in the cornea.

Topics: Animals; Aprotinin; Burns, Chemical; Cornea; Corneal Injuries; Drug Evaluation, Preclinical; Endopeptidases; Eye Burns; Glycoside Hydrolases; Rabbits; Sodium Hydroxide; Tears; Time Factors; Trypsin Inhibitors

The paper generalizes and analyses experimental results obtained in 60 eyes of 30 rabbits after induced burn by means of epichlorhydrine that is widely used in chemical industry and produces industrial burns of the eye in 20% of all burns in the town of Sumgait. Clinico-morphologic researches allowed to reveal peculiarities of pathogenesis of severe eye burns produced by epichlorhydrine causing toxic lesions of the iris and to substantiate effectiveness of hordox used in such cases, that has not only antiproteolytic, but also antiinflammatory, cytotoxic action on the burn process.

Topics: Accidents, Occupational; Animals; Aprotinin; Burns, Chemical; Drug Evaluation, Preclinical; Epichlorohydrin; Eye; Eye Burns; Rabbits; Time Factors; Trypsin Inhibitors

The present paper reports the results of a study on the use of a polyvalent proteolysis inhibitor (Gordox), the active agent of which is aprotinin, in early stages of ocular burn injuries. Following experimental corneal burns, Gordox influenced the activity of proteolytic enzymes in ocular tissue and morphologic changes in the cornea. Various administration methods were studied--instillation, and subconjunctival and intravenous injection. The preparation reduced the activity of proteolytic enzymes in the burned region of the cornea by a factor of two and led to "stages" in healing processes, thus preventing severe complications following ocular burns. In 98 patients (152 eyes) with ocular burn injuries, Gordox was found to be highly efficacious: in particular, it reduced the severity of inflammation in ocular tissues and considerably decreased the incidence of corneal perforations.

Topics: Animals; Aprotinin; Burns, Chemical; Cornea; Eye Burns; Humans; Ophthalmic Solutions; Rabbits; Trypsin Inhibitors; Wound Healing