apigenin and Smear-Layer

Endodontic success depends heavily on effective chemo-mechanical debridement of root canals through the use of instruments and irrigating solutions.. To compare the effectiveness of chamomile hydroalcoholic extract, Biopure™ mixture of tetracycline isomer, acid and detergent (MTAD) and 2.5 % sodium hypochlorite (NaOCl) on removal of the smear layer.. Randomised controlled trial.. Thirty extracted single-rooted, primary human teeth were allocated at random into three experimental groups of 10 teeth each. For each tooth, the canal was prepared using the step-back technique. During instrumentation, 2 ml of the irrigant was used for at least 10 s after each file and 10 ml as a final flush for 2 min for chamomile and NaOCL irrigants. Whereas for MTAD, an initial rinse with 1.3 % NaOCl for a cumulated period of 20 min, and use of MTAD as the final rinse for a period of 5 min was followed. Longitudinal grooves were made on root segments, then split into two halves with a chisel, stored in 2.5 % glutaraldehyde solution and fixed in ethanol series. Specimens were examined for the smear layer according to Hulsmann et al. (Int Endod J 35:668-679, 2002) criteria using a scanning electron microscope.. Kruskal-Wallis and Mann-Whitney U tests were used.. The most effective result in removal of smear layer occurred with the use of MTAD, followed by chamomile extract. The chamomile extract was found to be significantly more effective than 2.5 % NaOCl solution which had only minor effects.. The efficacy of chamomile to remove the smear layer was superior to 2.5 % NaOCl alone, but less effective than MTAD mixture.

Topics: Chamomile; Dental Pulp Cavity; Dentin; Detergents; Edetic Acid; Humans; Materials Testing; Matricaria; Microscopy, Electron, Scanning; Root Canal Irrigants; Root Canal Preparation; Smear Layer; Sodium; Sodium Hypochlorite; Statistics, Nonparametric; Tetracycline; Tooth Apex