aphidicolin has been researched along with Rhabdomyosarcoma--Alveolar* in 1 studies
1 other study(ies) available for aphidicolin and Rhabdomyosarcoma--Alveolar
Article | Year |
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Aphidicolin induces myogenic differentiation in the human rhabdomyosarcoma cell line KFR.
The effects of aphidicolin, a specific inhibitor of DNA polymerase alpha, on cell growth, DNA synthesis and myogenic differentiation in the human alveolar rhabdomyosarcoma cell line KFR were studied. The treatment with aphidicolin at 5 x 10(-6) M concentration, which completely inhibited DNA synthesis and cell growth, induced morphological differentiation of small mononuclear cells to elongated, multinucleated (myotube-like) structures. The morphological differentiation was accompanied by the expression of skeletal muscle myosin; about 30% myosin-positive cells were observed after 14 days of treatment, compared to 2.3% in untreated cultures. The results showed that aphidicolin induces differentiation of human rhabdomyosarcoma cells and that multinucleated myotube-like elements may develop simply by cell fusion without cell division and DNA synthesis. Topics: Aphidicolin; Cell Differentiation; Cell Division; Cell Survival; DNA Replication; Humans; Muscles; Myosins; Rhabdomyosarcoma, Alveolar; Tumor Cells, Cultured | 1994 |