ankaflavin and Breast-Neoplasms

Multireservoir nanocarriers were fabricated for delivering antineoplastic drug cocktail from herbal and fungal origin. Monascus yellow pigments (MYPs), monascin and ankaflavin, were isolated from red-mold rice, and incorporated within casein micelles (CAS MCs) along with the herbal drug, resveratrol (RSV). Both drugs (MYPs and RSV) were simultaneously incorporated into the hydrophobic core of CAS MCs. Alternatively, MYPs-loaded CAS MCs were enveloped within RSV-phytosomal bilayer elaborating multireservoir nanocarriers.. Cytotoxicity studies confirmed the superiority of multireservoir nanocarriers against MCF-7 breast cancer cells. The in vivo antitumor efficacy was revealed by reduction of the tumor volume and growth biomarkers.. Multireservoir CAS nanocarriers for codelivery of both MYPs and RSV may be promising alternative to traditional breast cancer therapy.

Topics: Animals; Antineoplastic Agents; Breast Neoplasms; Carcinoma, Ehrlich Tumor; Caseins; Cell Survival; Drug Carriers; Drug Therapy, Combination; Female; Flavins; Heterocyclic Compounds, 3-Ring; Humans; Hydrophobic and Hydrophilic Interactions; MCF-7 Cells; Mice, Inbred BALB C; Micelles; Monascus; Nanoparticles; Particle Size; Rats; Resveratrol

Monascin and ankaflavin, the major fractions of the fungal-derived monascus yellow pigments, were incorporated with the herbal drug, resveratrol (RSV) within the core of folate-conjugated casein micelles (FA-CAS MCs, F1) for active targeting. PEGylated RSV-phospholipid complex bilayer enveloping casein-loaded micelles (PEGPC-CAS MCs) were also developed as passive-targeted nanosystem.. FA- and PEGPC-CAS MCs demonstrated a proper size with monomodal distribution, sustained drug release profiles and good hemocompatibility. The coloaded MCs showed superior cytotoxicity to MCF-7 breast cancer cells compared with free drugs. Both nanosystems exerted excellent in vivo antitumor efficacy in breast cancer bearing mice with PEGylated MCs showing comparable tumor regression to folate-conjugated MCs.. Evergreen nanoplatforms coloaded with monascus yellow pigments and RSV were effective for breast cancer treatment.

Topics: Animals; Antineoplastic Agents; Breast Neoplasms; Caseins; Drug Carriers; Female; Flavins; Folic Acid; Heterocyclic Compounds, 3-Ring; Humans; MCF-7 Cells; Mice; Micelles; Polyethylene Glycols; Polymers; Resveratrol; Xenograft Model Antitumor Assays