angiotensinogen and Sleep-Apnea--Obstructive

Hypertension occurred in 50% obstructive sleep apnea-hypopnea syndrome (OSAHS) patients meanwhile OSAHS occurred in 30% hypertension patients. The present study aimed to explore the molecular mechanism of GATA2-EDN1-AGT induced hypertension in the development of obstructive sleep apnea-hypopnea syndrome. OSAHS patients (56 cases: 36 cases of male, 20 cases of female, 42~60 years old) were divided into two groups (case group: patients with hypertension monitored by 24 h ambulatory blood pressure and polysomnography; control group: patients without hypertension). Wistar rats were used to establish the OSAHS model (narrow pharyngeal cavity). PaO2 and PaCO2 of patients and rats were measured by an automatic blood gas analyzer. The profile of total protein in the OSAHS group and normal group was evaluated. Protein-protein-interaction (PPI) was carried out to show all matter proteins related. The levels of EDN-1, AGTII and atrial natriuretic peptide (ANP) in blood samples of patients and rats were analyzed by enzyme-linked immunosorbent assay (ELISA). The expression of GATA2, EDN1, endothelin-converting enzyme 1 (ECE-1) and AGTⅡ was measured. The results showed that SaO2 and AHI were positively associated with systolic pressure (P<0.05) in OSAHS patients. There was no correlation among other indexes (P>0.05). It was also observed that GATA2 had a strong relationship with AGTⅡ and EDN1. The results of ELISA presented that the levels of EDN1, AGTⅡ and ANP in the OSAHS group of human and animal models were significantly increased (P<0.05). The results of immunochemistry showed that the expression of GATA2 and AGTⅡ in the vascular of OSAHS group was upregulated manifestly (P<0.05). It was concluded that OSAHS can induce AHI, which increases hypertension via the GATA2-EDN1-AGT Ⅱ axis.

Topics: Angiotensinogen; Animals; Atrial Natriuretic Factor; Blood Pressure Monitoring, Ambulatory; Endothelin-1; Female; GATA2 Transcription Factor; Hypertension; Male; Rats; Rats, Wistar; Sleep Apnea, Obstructive; Syndrome

To investigate the association of polymorphisms in the angiotensin system gene with obstructive sleep apnea-hypopnea syndrome (OSAHS).. The genotypes and alleles of angiotensin-converting enzyme (ACE) and angiotensinogen (AGT) genes were identified by polymerase chain reaction-restricted fragment length polymorphism assay in 221 unrelated subjects of the north region "Han" population of China (including 121 OSAHS subjects and 100 non-OSAHS subjects). The genotypes and allele frequencies of the polymorphisms were compared between OSAHS group and non-OSAHS group. The effects of the polymorphisms in OSAHS group on body mass index (BMI), neck circumference (NC), waist/hip rate (WHR), apnea-hypopnea index (AHI), systolic blood pressure (SBP), and diastolic blood pressure (DBP) were analysed.. There were significant differences in genotypes distribution in AGT polymorphism between the two groups (P = 0.009). Compared with the control group, OSAHS group had significantly higher T allele frequency in AGT polymorphism (P = 0.020). There were independent effects of AGT polymorphism on BMI, NC, WHR in OSAHS group, and carriers of the T allele of AGT polymorphism had greater BMI, NC, and WHR, while the carriers of the T allele had greater AHI, SBP, and DBP.. ACE polymorphism may not be correlated with central obesity and OSAHS in the population. AGT polymorphism may be involved in the development of central obesity and may be related to OSAHS and hypertension in OSAHS patients by the central obesity in male OSAHS subjects of North region "Han" population of China.

Topics: Adult; Angiotensinogen; Angiotensins; Blood Pressure; Gene Frequency; Genotype; Humans; Male; Obesity; Peptidyl-Dipeptidase A; Polymerase Chain Reaction; Polymorphism, Genetic; Sleep Apnea, Obstructive