angiotensinogen and Eclampsia

The angiotensinogen gene M235T polymorphism is related to an increased risk of hypertension. Hypertension and pregnancy-induced hypertension have been suggested to share common etiologic factors. We examined whether this mutation also increases the risk of preeclampsia/eclampsia.. Pubmed/Medline, Web of Science and EMBASE were searched and a hand search of bibliographies was conducted. In all, 17 studies (including 1446 cases and 3829 controls) published in English between 1993 and October 2006 on the association of angiotensinogen gene M235T polymorphism with preeclampsia/eclampsia were selected.. The overall odds ratio (OR) under a random effects model revealed that individuals homozygous for the T allele were 1.62 times more likely to develop preeclampsia/eclampsia [95% confidence interval (CI), 1.12 to 2.33; P = 0.01) compared to individuals homozygous for the M allele. The relation in Caucasians (OR = 1.99; 95% CI, 1.18-3.36; P = 0.01) was similar to that in East Asian populations (OR = 1.74; 95% CI, 0.92-3.28; P = 0.09), although the latter was not statistically significant due to lower numbers of studies. Under additive, recessive and dominant genetic models positive associations were also found. A meta-regression analysis showed that ethnic background was a significant source of between-study heterogeneity (P = 0.04) but design of the study, study size and Hardy-Weinberg equilibrium deviation were not. There was a low probability of publication bias.. Our meta-analysis expands the findings on hypertension by showing that the presence of the T allele of the angiotensinogen gene is associated with an increased risk to develop preeclampsia/eclampsia.

Topics: Angiotensinogen; Eclampsia; Female; Genetic Predisposition to Disease; Humans; Hypertension, Pregnancy-Induced; Polymorphism, Genetic; Pre-Eclampsia; Pregnancy; Regression Analysis

To analyze methylation profiles of known preeclampsia/eclampsia (PE) candidate genes in normal (NL) and preeclamptic (PE) women at delivery.. A matched case-control study comparing methylation in 79 CpG sites/33 genes from an independent gene set in maternal leukocyte DNA in PE and NL (n = 14 each) on an Illumina BeadChip platform. Replication performed on second cohort (PE = 12; NL = 32).. PE demonstrates differential methylation in POMC, AGT, CALCA, and DDAH1 compared with NL.. Differential methylation in four genes associated with PE may represent a potential biomarker or an epigenetic pathophysiologic mechanism altering gene transcription.

Topics: Adult; Amidohydrolases; Angiotensinogen; Calcitonin Gene-Related Peptide; Case-Control Studies; CpG Islands; DNA Methylation; Eclampsia; Female; Gene Expression Profiling; Humans; Leukocytes; Parturition; Pre-Eclampsia; Pregnancy; Pro-Opiomelanocortin; Young Adult

Preeclampsia/eclampsia (PE/E) is a common disease of human pregnancy with a strong genetic component. The etiology of PE/E is unknown. Two recent reports indicated that the angiotensinogen gene (AGT) could be involved in susceptibility to PE/E. We performed a population-based case-control study in Australian and Chinese populations to investigate whether AGT is a good candidate gene for PE/E. A microsatellite polymorphism within AGT was typed as well as a molecular variant T235 (Met-->Thr) of AGT using allele-specific PCR and allele-induced restriction site PCR. The allele distributions of the microsatellite and the variant T235 of AGT were significantly different between the two ethnic groups. However, no significant allele associations were found with disease when comparing PE/E patients and controls in Australian or Chinese populations, which is in contrast to the two earlier reports. The results suggest that the contribution of AGT to the occurrence of PE/E is small, if anything, and is not constant across populations.

Topics: Alleles; Angiotensinogen; Australia; Case-Control Studies; China; Eclampsia; Female; Gene Frequency; Genetic Variation; Humans; Minisatellite Repeats; Polymerase Chain Reaction; Pre-Eclampsia; Pregnancy

Topics: Angiotensinogen; Asian People; Eclampsia; Female; Gene Frequency; Genotype; Humans; Indonesia; Japan; Polymorphism, Genetic; Pre-Eclampsia; Pregnancy

Topics: Adult; Angiotensin II; Angiotensinogen; Animals; Birth Weight; Cardiovascular Diseases; Cephalometry; Eclampsia; Embryonic and Fetal Development; Female; Humans; Hypertension; Infant, Newborn; Pregnancy; Pregnancy Complications, Cardiovascular

Topics: Angiotensinogen; Eclampsia; Female; Genotype; Humans; Hypertension; Pre-Eclampsia; Pregnancy; Pregnancy Complications, Cardiovascular; Pregnancy in Diabetics