angiotensin-i and Sarcoidosis

Topics: Angiotensin I; Angiotensins; Humans; Lung Diseases; Molecular Weight; Peptidyl-Dipeptidase A; Sarcoidosis

Topics: Angiotensin I; Blood Circulation; Gaucher Disease; Humans; Kallikreins; Kinins; Peptidyl-Dipeptidase A; Renin; Sarcoidosis

Serum ACE (s-ACE) activity were determined in 112 sarcoid patients and 60 healthy volunteers. The mean s-ACE activity in sarcoid patients were 39.5 +/- 14.1 U. and in healthy volunteers 22.1 +/- 3.6, U. Significant increase of s-ACE was seen in active sarcoidosis 51.2 +/- 13.7 U. Serum ACE activity increased twice or more in comparison with control values imply activity of the process. Serum-ACE determination may be of use as a prognostic factor in sarcoidosis.

Topics: Adolescent; Adult; Angiotensin I; Female; Humans; Lung Diseases; Male; Middle Aged; Peptidyl-Dipeptidase A; Prognosis; Reference Values; Sarcoidosis; Severity of Illness Index

The authors studied the parameters of Friedland and Silverstein's technique for measuring the activity of the angiotensin I conversion enzyme and found a number of anomalies. The technique was modified by correcting the wide pH difference between the pH of the reagent and the pH of the reference solution. This modification made the technique optimal for the measurement of the enzyme activity by allowing a 20 to 40 per cent increase in this activity and a 15% increase in the positivity in cases of clinical sarcoidosis. The application of this modified test to the determination of the activity of the angiotension I conversion enzyme in control subjects, patients with sarcoidosis and patients with order respiratory diseases gave the following mean values, respectively: 39 +/- 10 IU; 81 +/- 23 IU; 41.5 +/- 2.5 IU in the serum and 0.2 IU; 1.1 +/- 0.5 IU; 0.2 +/- 0.1 IU in bronchoalveolar lavage fluid. The increase in enzyme activity in sarcoidosis is found in the serum in 75% of cases and in bronchoalveolar lavage fluid in 85% of cases. This modified technique can be applied in routine practice and in research.

Topics: Angiotensin I; Angiotensins; Humans; Hydrogen-Ion Concentration; In Vitro Techniques; Indicators and Reagents; Methods; Peptidyl-Dipeptidase A; Phosphates; Respiratory Tract Diseases; Sarcoidosis; Spectrometry, Fluorescence

Topics: Angiotensin I; Female; Humans; Male; Peptidyl-Dipeptidase A; Sarcoidosis