angiogenin and Stomach-Neoplasms

To investigate the implication of angiogenin (ANG) in the neovascularizaton and growth of human gastric carcinoma (HGC).. ANG mRNA expression in HGC specimens obtained by surgical resection from patients with HGC were examined by RT-PCR. ANG, Ki-67, VEGF protein expression and microvessel density (MVD) in HGC specimens were detected by immunohistochemistry.. RT-PCR showed significantly higher ANG mRNA expression (0.482 +/- 0.094) in HGC tissues than in the surrounding nontumorous tissues (0.276 +/- 0.019, P = 0.03). MVD within tumorous tissues increased significantly with ANG mRNA expression (r = 0.380, P = 0.001) and ANG protein expression (P < 0.01). The ANG expression levels of cancer tissues were positively correlated with VEGF (P < 0.01) and the proliferation index of cancer cells (P < 0.01).. ANG is one of the neovascularization factors of HGC. ANG may work in coordination with VEGF, and promote the proliferation of HGC cells.

Topics: Aged; Cell Proliferation; Female; Gene Expression Regulation, Neoplastic; Humans; Immunohistochemistry; Ki-67 Antigen; Male; Microcirculation; Middle Aged; Neovascularization, Pathologic; Reverse Transcriptase Polymerase Chain Reaction; Ribonuclease, Pancreatic; RNA, Messenger; Stomach Neoplasms; Vascular Endothelial Growth Factor A

Angiogenin (ANG), an efficient angiogenesis factor, is up-regulated in human colorectal carcinoma, pancreatic carcinoma, and other tumors. But the function of ANG in tumor angiogenesis is still unknown. This study was to explore the effect of ANG on angiogenesis of gastric carcinoma.. The expressions of ANG, CD34, and Ki-67 in 68 specimens of gastric carcinoma were detected by immunohistochemistry. Microvessel density (MVD) was figured out according to the expression of CD34. The proliferation index of carcinoma cells in gastric carcinoma tissue was figured out according to the expression of Ki-67.. Of the 68 specimens of gastric carcinoma, 19 showed strongly positive expression of ANG (group A), 3 showed negative expression of ANG (group B). MVD of group A was significantly higher than that of group B (308.4+/-25.6 vs. 196.0+/-31.3, P<0.01). Proliferation index of group A was significantly higher than that of group B (579.6+/-31.4 vs. 341.3+/-84.0, P<0.01).. Protein level of ANG positively correlates with MVD of gastric carcinoma tissue, and proliferation index of carcinoma cells.

Topics: Adenocarcinoma; Antigens, CD34; Cell Proliferation; Female; Humans; Ki-67 Antigen; Lymphatic Metastasis; Male; Microcirculation; Middle Aged; Neoplasm Staging; Neovascularization, Pathologic; Ribonuclease, Pancreatic; Stomach Neoplasms

The growth behaviour of well-differentiated neuroendocrine carcinomas of the gastro-entero-pancreatic system varies greatly and parameters predicting their prognosis are lacking. The aim of our study was to investigate whether tumour growth could be correlated with the release of proangiogenic factors into the circulation.. Circulating vascular endothelial growth factor (VEGF), interleukin-8 (IL-8), basic fibroblast growth factor (bFGF) and angiogenin were measured in 38 patients with advanced neuroendocrine carcinomas and compared to healthy age-matched controls. In 20 patients, angiogenic cytokine levels were measured at consecutive time points and correlated to tumour progression as assessed by abdominal CT scan, MRI and chromogranin A levels.. VEGF levels were elevated in patients compared to controls (P < 0.002) and clearly associated with tumour progression (P < 0.005). Angiogenin levels were significantly higher in patients than in controls (P < 0.003), while high IL-8 levels were predictive of shorter survival. Angiogenin and bFGF levels were correlated neither with tumour growth nor with patient survival.. VEGF and IL-8 are associated with tumour progression and might qualify as markers of prognosis and therapy control in patients with neuroendocrine carcinomas. Our results support the notion that specific anti-angiogenic therapies should be evaluated in neuroendocrine carcinoma patients.

Topics: Adult; Aged; Angiogenic Proteins; Biomarkers, Tumor; Case-Control Studies; Chi-Square Distribution; Female; Fibroblast Growth Factor 2; Humans; Interleukin-8; Intestinal Neoplasms; Male; Middle Aged; Neoplasm Staging; Neuroendocrine Tumors; Pancreatic Neoplasms; Predictive Value of Tests; Ribonuclease, Pancreatic; Stomach Neoplasms; Survival Rate; Vascular Endothelial Growth Factor A

The purpose of this study is to elucidate an increased expression of angiogenin (ANG) as a prognostic factor of gastric cancer (GC), against the background of our previous observations of the increased expression of ANG in the more progressed GC.. We investigated serum ANG concentrations in 123 GC patients and 63 healthy volunteers as well as the distributions of ANG gene message in 52 GC tissues by in situ hybridization. The prognostic significance of ANG was investigated by the Cox proportional hazards model including variable selection and by survival analysis.. The mean serum ANG concentrations in GC patients (378.3+/-95.5 ng/ml) were significantly higher (P=0.0001) than those in the healthy volunteers (334.1+/-58.2 ng/ml). Either strong, moderate, weak, or no ANG gene message expression was seen in 25, 22, 4, and 1 patients, respectively, in GC cells as well as in interstitial cells in the vicinity of cancer cells, a finding in accord with our previous results of ANG protein localization. The variable selection method selected increased (> or =400 ng/ml) serum ANG concentration (P=0.02), undifferentiated histological type (P=0.01), cancer depth (P=0.001), and third-tier lymph node involvement (P=0.0005) as an independent prognostic factor by the Cox proportional hazards model. A significant correlation was seen between higher serum ANG concentrations (> or =400 ng/ml) and worse disease-free (P=0.003) or disease-specific (P=0.03) survivals. CONCLUSIONS. These results suggest that serum levels of ANG are an independent prognostic factor that could be a predictor of postoperative outcomes of GC patients.

Topics: Adult; Aged; Disease Progression; Female; Humans; Male; Middle Aged; Multivariate Analysis; Prognosis; Ribonuclease, Pancreatic; Stomach Neoplasms

The purpose of this study is to elucidate the expression of angiogenin and its previously undemonstrated clinical significance in gastric cancer (GC).. Angiogenin expression was examined immunohistochemically in 21 GC tissues and 21 corresponding normal gastric tissues. The serum concentration was determined by enzyme-linked immunosorbent assay (ELISA) in GC patients preoperatively (n = 48) and postoperatively (n = 41), in nonneoplastic patients preoperatively (n = 23) and postoperatively (n = 19), and in 32 healthy volunteers. The amount of angiogenin in the tissue of 21 GC patients was also determined by ELISA.. Angiogenin expression was observed in GC cells as well as in some fundic glandular cells and some inflammatory cells. The mean serum concentration in GC patients (407.8 +/- 105.2 ng/ml) was significantly higher than that in the nonneoplastic patients (345.7 +/- 58.3 ng/ml; P < 0.003) and in the healthy volunteers (333.0 +/- 59.3 ng/ml; P < 0.0002). The mean serum angiogenin concentrations were progressively higher in the order T1 + T2 (P < 0.04) < T3 + T4 (P < 0.0001) < recurrent GC (P < 0.05) subgroups, in the order node-negative (P < 0.05) < node-positive (P < 0.0002) subgroups, and in the order stage I +II (P < 0.02) < stage III and over (P < 0.0005) subgroups as compared with those in the healthy volunteers. These elevated serum angiogenin concentrations in each subgroup were significantly (P < 0.0003) reduced after cancer resection. The amounts of angiogenin in GC tissues correlated significantly with the serum angiogenin concentration (P < 0.01).. These results suggest that angiogenin expression is increased in GC and that the increased serum concentration in GC patients correlates with cancer progression.

Topics: Adult; Angiogenesis Inducing Agents; Case-Control Studies; Disease Progression; Enzyme-Linked Immunosorbent Assay; Female; Humans; Immunohistochemistry; Male; Neoplasm Proteins; Neovascularization, Pathologic; Ribonuclease, Pancreatic; Statistics, Nonparametric; Stomach Neoplasms

Using digoxigenin-labelled, synthetic oligonucleotide probe cocktails of angiogenin and bFGF genes, the expression of the two genes was observed by in situ hybridization in ten colonic adenocarcinomas, seven gastric adenocarcinomas, and four hepatocellular carcinomas. The angiogenin gene was expressed in eight of the ten cases of colonic adenocarcinoma and in all of the four cases where dysplastic glands were found. Angiogenin expression was evident in four of the seven cases of gastric adenocarcinoma. bFGF expression was detected in only five of the seven gastric carcinoma cases. The mRNAs for angiogenin and bFGF were mainly cytoplasmic in distribution and were only occasionally seen in the nuclei of the positive cells. Neither the angiogenin gene mRNA nor the bFGF mRNA was expressed in the four cases of hepatocellular carcinoma. It is postulated that the angiogenin gene may play an important role in angiogenesis in colonic adenocarcinomas; in gastric cancers, both angiogenin and bFGF were involved in this process. For hepatocellular carcinomas, neither angiogenin nor bFGF production appeared to be related to angiogenesis.

Topics: Adenocarcinoma; Angiogenesis Inducing Agents; Carcinoma, Hepatocellular; Colonic Neoplasms; Fibroblast Growth Factor 2; Gastrointestinal Neoplasms; Humans; In Situ Hybridization; Liver Neoplasms; Neoplasm Proteins; Neovascularization, Pathologic; Proteins; Ribonuclease, Pancreatic; RNA, Messenger; Stomach Neoplasms