an-207 and Leukopenia

We determined by immunohistochemistry the presence of LHRH receptors in surgical specimens of human non-Hodgkin's lymphomas (NHL) and investigated the expression of LHRH receptors in two human NHL cell lines, RL and HT by RT-PCR, Western blot and radioligand binding studies. In in vivo experiments with nude mice bearing tumours of these NHL cell lines, the efficacy of cytotoxic LHRH analogue AN-207 and its cytotoxic radical AN-201 was examined. LHRH receptors were detected in 94.1% of the human NHL specimens and in both NHL cell lines. AN-207 significantly (P < 0.01) inhibited the growth of RL and HT tumours, while the non-targeted AN-201 had no effects. Blockade of the LHRH receptors with an excess of LHRH agonist Decapeptyl suppressed the antitumour effects of AN-207. Our findings indicate that LHRH receptors expressed in a high percentage of human NHL specimens can be used for effective targeted therapy with the cytotoxic LHRH analogue AN-207.

Topics: Animals; Cell Line, Tumor; Doxorubicin; Female; Gonadotropin-Releasing Hormone; Humans; Immunohistochemistry; Leukopenia; Lymphoma, Non-Hodgkin; Mice; Mice, Nude; Neoplasm Transplantation; Receptors, LHRH; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Weight Loss